한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제19권2호
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  • Pages.79-84
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  • 2015
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer

  • Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keun Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Chung, Hak-Jae (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Cho, In-Cheol (Subtropical Livestock Research Institute, NIAS) ;
  • Lee, Jeong-Woong (Functional Genomics Research Center, KRIBB) ;
  • Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 투고 : 2015.04.05
  • 심사 : 2015.05.20
  • 발행 : 2015.06.30
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초록

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

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