Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Characterization of α-D-manosidase activity from Bacillus safensis MA-01

Bacillus safensis MA-01 유래 알파-만노사이데이즈의 효소학적 특성

  • Lee, Bo Mi (Department of Life Sciences, Gachon University) ;
  • Kim, Joo Won (Department of Life Sciences, Gachon University) ;
  • Park, Jae Kweon (Department of Life Sciences, Gachon University)
  • 이보미 (가천대학교 바이오나노대학 생명과학과) ;
  • 김주원 (가천대학교 바이오나노대학 생명과학과) ;
  • 박제권 (가천대학교 바이오나노대학 생명과학과)
  • Received : 2015.06.15
  • Accepted : 2015.06.30
  • Published : 2015.06.30
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Abstract

An extracellular alkaline ${\alpha}$-D-mannosidase produced by a strain named as MA-01 was produced and its preliminary enzyme activity was characterized. Upon determining the 16S rDNA sequence and its homology search, the strain was identified to be one of species of the Bacillus safensis. Localization of enzyme was elucidated that ${\alpha}$-D-mannosidase can be found in culture medium as an extracellular enzyme. In addition, partial enzyme activity of 63% compared with the extracellular enzyme activity was observed in membrane protein. The optimal pH and temperature of the ${\alpha}$-D-mannosidase were pH 7.5 and $37^{\circ}C$, respectively. The $K_m$ and $V_{max}$ values of the ${\alpha}$-D-mannosidase in crude enzyme toward p-nitrophenyl-${\alpha}$-D-mannopyranoside were determined to be $455.6{\mu}M$ and $10.8{\mu}mole/min/mg$ of protein, respectively. To the best of our knowledge, this is the first report described the alkaline ${\alpha}$-D-mannosidase from the family of B. safensis.

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References

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