Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Rubus Coreanus Miq. Oil on Serum Lipids in C57BL/6J Mice

복분자씨유의 식용유지 대체가 C57BL/6J Mice의 혈청 지질에 미치는 영향

  • Byun, Moon-Sun (Obesity Research Center, Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Cha, Youn-Soo (Obesity Research Center, Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Hwang, Keum-Taek (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University) ;
  • Yu, Ok-Kyeong (Obesity Research Center, Department of Food Science and Human Nutrition, Chonbuk National University)
  • 변문선 (전북대학교부설 비만연구센터, 전북대학교 식품영양학과) ;
  • 차연수 (전북대학교부설 비만연구센터, 전북대학교 식품영양학과) ;
  • 황금택 (서울대학교 생활과학대학 식품영양학과, 생활과학연구소) ;
  • 유옥경 (전북대학교부설 비만연구센터, 전북대학교 식품영양학과)
  • Received : 2015.03.06
  • Accepted : 2015.05.14
  • Published : 2015.07.31
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Abstract

This study evaluated the effects of Rubus coreanus Miq. oil on the plasma lipid profile of high fat diet (HFD)-induced obese mice. Animals were randomly divided into 4 groups (n=10). After completion of the 5-week experimental period, we measured bodyweight gain, food intake, adipose tissue mass, and plasma lipid profile. We also analyzed the activities of carnitine and superoxide dismutase (SOD) involved in ${\beta}$-oxidation and antioxidation, respectively. Our results show that HFD-induced weight gain in animals in the R. coreanus Miq. oil diet group (RCO) and corn oil diet group (CO) was significantly lower compared to animals in the HFD group; RCO supplementation had a more noticeable effect than CO. Visceral and back fat weights were lower in the RCO and CO groups while plasma HDL cholesterol (HDL-C) and HDL-C per total cholesterol [HDL-C/TC (%)] ratio were significantly higher in the RCO group. The contents of acid-soluble acylcarnitine and total carnitine as well as SOD activation were significantly higher in the RCO group, but no significant difference was observed between the RCO and CO groups. In conclusion, RCO effectively averted elevation of total body weight and fat weight in HFD-induced obese mice and promoted increased HDL-C. Therefore, R. coreanus Miq. oil might play an anti-obesity role in obese people and could be used as an effective oil supplement.

본 연구는 식이로 비만이 유도되는 C57BL/6J mice에 복분자씨유와 지방의 종류를 달리한 식이를 급여하여 체중 및 체내 지질패턴에 미치는 효과를 평가하고자 수행되었다. 이를 위해 실험동물의 체중증가량 및 식이섭취효율을 평가하고, 지방 조직의 무게 및 혈중과 간 중 지질성분을 분석하였다. 또한 ${\beta}$-oxidation과 관련된 카르니틴 함량 변화와 superoxide dismutase(SOD) 항산화효소 활성도를 측정하였다. 실험 결과 실험동물의 체중이 고지방복분자씨유첨가식이군(RCO군)에서 유의하게 감소하였고, 식이섭취효율이 고지방옥수수유첨가식이군(CO군)과 RCO군에서 유의적으로 낮았다. 복부 지방과 등 지방은 CO군과 RCO군에서 유의적으로 낮았고, 혈중 HDL-C와 HDL-C/TC(%)는 RCO군에서 유의적으로 높았다. 간 중 acid-soluble acylcarnitine 및 total carnitine의 카르니틴 수준은 CO군과 RCO군에서, SOD 항산화효소 활성은 RCO군에서 고지방돈지첨가식이군과 고지방우지첨가식이군에 비해 유의적으로 높았다. 본 연구 결과 복분자씨유는 비만이 유도된 성인 쥐의 체중 및 체지방량과 HDL-C 및 HDL-C/TC(%)의 개선에 긍정적인 영향을 미치는 것으로 분석되었으며, 이는 복분자씨유를 비만인들에게 적용했을 때 매우 효과적인 유지 급원으로써의 사용 가능성이 있음을 시사하는 결과로 사료된다. 이에 대해 추후 복분자씨유의 기능성과 상업성에 대한 후속 연구가 필요할 것으로 여겨지며, 본 연구는 현재 식품가공 과정중 폐기처분되고 있는 복분자씨가 식품산업의 새로운 소재로써의 가능성이 있음을 확인한 의미 있는 실험이라 사료된다.

Keywords

References

  1. Byamukama R, Kiremire BT, Andersen OM, Steigen A. 2005. Anthocyanins from fruits of Rubus pinnatus and Rubus rigidus. J Food Compos Anal 18: 599-605. https://doi.org/10.1016/j.jfca.2004.04.007
  2. Park YS, Jang HG. 2003. Natural products, organic chemistry: lactic acid fermentation and biological activities of Rubus coreanus. J Korean Soc Agric Chem Biotechnol 46: 367-375.
  3. Cha HS, Youn AR, Park PJ, Choi HR, Kim BS. 2007. Comparison of physiological activities of Rubus coreanus Miquel during maturation. J Korean Soc Food Sci Nutr 36: 683-688. https://doi.org/10.3746/jkfn.2007.36.6.683
  4. Cha HS, Lee MK, Hwang JB, Park MS, Park KM. 2001. Physicochemical characteristics of Rubus coreanus Miquel. J Korean Soc Food Sci Nutr 30: 1021-1025.
  5. Kwon KH, Cha WS, Kim DC, Shin HJ. 2006. A research and application of active ingredients in Bokbunja (Rubus coreanus Miquel). Korean J Biotechnol Bioeng 21: 405-409.
  6. Choung MG, Lim JD. 2012. Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J Mediclinal Crop Sci 20: 259-269. https://doi.org/10.7783/KJMCS.2012.20.4.259
  7. Ministry of Agriculture Food and Rural Affairs. 2014. 2013 an actual output of crop for a special purpose. http://ebook.mafra.go.kr/preview/viewer/main.php?site=2&menuno=2&previewno=6676&iframe=0&dlbt= (accessed Feb 2015).
  8. Shin JS, Cho EJ, Choi HE, Seo JH, An HJ, Park HJ, Cho YW, Lee KT. 2014. Anti-inflammatory effect of a standardized triterpenoid-rich fraction isolated from Rubus coreanus on dextran sodium sulfate-induced acute colitis in mice and LPS-induced macrophages. J Ethnopharmacol 2: 219-300.
  9. Choe M, Sin GJ, Choe GP, Do JH, Kim JD. 2003. Synergistic effects of extracts from Korean red ginseng, Saururus chinensis (Lour.) Baill. and Rubus coreanus Miq. on antioxidative activities in rats. Korean J Medicinal Crop Sci 11: 148-154.
  10. Oh HH. 2007. Chemical characteristics of raspberry and blackberry fruits produced in Korea. MS Thesis. Chonbuk National University, Jeonju, Korea.
  11. Ku CS, Mun SP. 2008. Characterization of seed oils from fresh Bokbunja (Rubus coreanus Miq.) and wine processing waste. Bioresour Technol 99: 2852-2856. https://doi.org/10.1016/j.biortech.2007.06.005
  12. Hands ES. 1996. Lipid composition of selected foods. In Bailey's Industrial Oil and Fat Products: Edible Oil and Fat Products. John Wiley & Sons Inc., New York, NY, USA. p 441-505.
  13. Hwang JT, Kang HC, Kim TS, Park WJ. 1999. Lipid componant and properties of grape seed oils. Korean J Food & Nutr 12: 150-155.
  14. Beynen AC, Katan MB. 1985. Why do polyunsaturated fatty acids lower serum cholesterol? Am J Clin Nutr 42: 560-563.
  15. Grundy SM, Ahrens Jr EH. 1970. The effects of unsaturated dietary fats on absorption, excretion, synthesis, and distribution of cholesterol in man. J Clin Invest 49: 1135-1152. https://doi.org/10.1172/JCI106329
  16. Spritz N, Ahrens Jr EH, Grundy S. 1965. Sterol balance in man as plasma cholesterol concentrations are altered by exchanges of dietary fats. J Clin Invest 44: 1482-1493. https://doi.org/10.1172/JCI105255
  17. Nestel PJ, Havenstein N, Whyte HM, Scott TJ, Cook LJ. 1973. Lowering of plasma cholesterol and enhanced sterol excretion with the consumption of polyunsaturated ruminant fats. N Engl J Med 288: 379-382. https://doi.org/10.1056/NEJM197302222880801
  18. Field CJ, Angel A, Clandinin MT. 1985. Relationship of diet to the fatty acid composition of human adipose tissue structural and stored lipids. Am J Clin Nutr 42: 1206-1220.
  19. Kim CO, Kang SA. 2001. Effect of high fat and high carbohydrate diet on serum leptin and lipids concentration in rat. Korean J Nutr 34: 123-131.
  20. Reeves PG, Nielsen FH, Fahey Jr GC. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939- 1951.
  21. Ryu MH, Sohn HS, Heo YR, Moustaid-Moussa N, Cha YS. 2005. Differential regulation of hepatic gene expression by starvation versus refeeding following a high-sucrose or highfat diet. Nutrition 21: 543-552. https://doi.org/10.1016/j.nut.2005.01.001
  22. Cederblad G, Lindstedt S. 1972. A method for the determination of carnitine in the picomole range. Clin Chim Acta 37: 235-243. https://doi.org/10.1016/0009-8981(72)90438-X
  23. Sachan DS, Rhew TH, Ruark RA. 1984. Ameliorating effects of carnitine and its precursors on alcohol-induced fatty liver. Am J Clin Nutr 39: 738-744.
  24. Okado-Matsumoto A, Fridovich I. 2001. Subcellular distribution of superoxide dismutases (SOD) in rat liver: Cu, Zn-SOD in mitochondria. J Biol Chem 276: 38388-38393. https://doi.org/10.1074/jbc.M105395200
  25. Park YJ, Park YJ. 1997. Effect of high fat and high cholesterol diet on kidney function. J Nutr Health 30: 187-194.
  26. Morley JE. 2001. Decreased food intake with aging. J Gerontol A Biol Sci Med Sci 56: 81-88. https://doi.org/10.1093/gerona/56.suppl_2.81
  27. Osborne TB, Mendel LB, Ferry EL. 1917. The effect of retardation of growth upon the breeding period and duration of life of rats. Science 23: 294-295.
  28. Dodge JA. 1994. Dietary fats and gastrointestinal function. Eur J Clin Nutr 48: S8-S16.
  29. Kang TS, Lee MY, Beak SH, Jeong HS, Park HJ, Kong YJ, Jung IS. 2005. Effects of oat soluble ${\beta}$-glucan on glucose dialysis retardation and blood glucose in diabetic rats. Food Engineering Progress 9: 88-96.
  30. Kim ID, Kang KS, Kwon RH, Yang JO, Lee JS, Ha BJ. 2007. The effect of Rubus coreanum Miquel against lipopolysaccharide- induced oxidative stress and lipid metabolism. J Fd Hyg Safety 22: 213-217.
  31. Choi HM. 2004. Nutrition. Kyomunsa, Seoul, Korea. p 90-94.
  32. Choi MJ, Jo HJ. 2005. Effects of isoflavones supplemented diet on lipid concentrations and hepatic LDL receptor mRNA level in growing female rats. Korean J Nutr 35: 344-351.
  33. Bieber LL. 1988. Carnitine. Ann Rev Biochem 57: 261-283. https://doi.org/10.1146/annurev.bi.57.070188.001401
  34. Bremer J. 1983. Carnitine-metabolism and functions. Physiol Rev 63: 1420-1480.
  35. Cha YS. 1993. Cellular and enzymatic basis for carnitinemediated attenuation of ethanol metabolism. MS Thesis. Tennessee University, Knoxville, TN, USA.
  36. Cha YS, Sohn HS, Daily III JW, Oh SH. 1999. Effects of exercise training and/or high fat diet on lipid metabolism and carnitine concentrations in rats. Nutr Res 19: 934-945.
  37. Cha YS, Choi SK, Suh H, Lee SN, Cho D, Li K. 2001. Effects of carnitine coingested caffeine on carnitine metabolism and endurance capacity in athletes. J Nutr Sci Vitaminol (Tokyo) 47: 378-384. https://doi.org/10.3177/jnsv.47.378
  38. Fantos JC, Ward PA. 1982. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 107: 397-418.
  39. Bergsten P, Amitai G, Kehrl J, Levine M. 1990. Ascorbic acid content of human B and T lymphocytes and monocytes. Ann NY Acad Sci 587: 275-277. https://doi.org/10.1111/j.1749-6632.1990.tb00155.x
  40. Ames BN, Shigenaga MK, Hagen TM. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 90: 7915-7922. https://doi.org/10.1073/pnas.90.17.7915
  41. Epe B. 1991. Genotoxocity of singlet oxygen. Chem Biol Interact 80: 239-260. https://doi.org/10.1016/0009-2797(91)90086-M
  42. Pryor WA, Stone K. 1993. Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann NY Acad Sci 686: 12-27. https://doi.org/10.1111/j.1749-6632.1993.tb39148.x
  43. Ji LL. 1995. Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med 18: 1079-1086. https://doi.org/10.1016/0891-5849(94)00212-3
  44. Gutteridge JMC, Halliwell B. 1994. Antioxidants in nutrition, health and disease. Oxford University Press, Oxford, UK. p 143.
  45. Pieszka M, Tombarkiewicz B, Roman A, Migdal W, Niedziolka J. 2013. Effect of bioactive substances found in rapeseed, raspberry and strawberry seed oils on blood lipid profile and selected parameters of oxidative status in rats. Environ Toxicol Pharmacol 36: 1055-1062. https://doi.org/10.1016/j.etap.2013.09.007
  46. Yoon I, Cho JY, Kuk JH, Wee JH, Jang MY, Ahn TA, Park KH. 2002. Identification and activity of antioxidative compounds from Rubus coreanum fruit. Korean J Food Sci Technol 34: 898-904.
  47. Lee JW, Do JH. 2000. Determination of total phenolic compounds from the fruit of Rubus coreanum and antioxidative activity. J Korean Soc Food Sci Nutr 25: 943-947.