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Whitening Effect of Octaphlorethol A Isolated from Ishige foliacea in an In Vivo Zebrafish Model

  • Kim, Kil-Nam (Jeju Center, Korea Basic Science Institute (KBSI)) ;
  • Yang, Hye-Mi (Department of Marine Life Science, Jeju National University) ;
  • Kang, Sung-Myung (Department of Marine Life Science, Jeju National University) ;
  • Ahn, Ginnae (Department of Marine Bio-Food Sciences, Chonnam National University) ;
  • Roh, Seong Woon (Jeju Center, Korea Basic Science Institute (KBSI)) ;
  • Lee, WonWoo (Department of Marine Life Science, Jeju National University) ;
  • Kim, Daekyung (Jeju Center, Korea Basic Science Institute (KBSI)Jeju Center, Korea Basic Science Institute (KBSI)) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
  • Received : 2014.09.12
  • Accepted : 2014.10.22
  • Published : 2015.04.28

Abstract

In a previous study, we isolated octaphlorethol A (OPA) from Ishige foliacea and evaluated its anti-melanogenesis activity in a murine melanoma cell line. However, the whitening effect and toxicity of OPA have not yet been examined in vivo. Therefore, in this study, we investigated the inhibitory effect of OPA on melanin synthesis and tyrosinase activity in an in vivo zebrafish model. More than 90% of subject embryos survived upon exposure to OPA concentrations below $25{\mu}M$, which was not significantly different from the finding in the control group. OPA markedly inhibited melanin synthesis and tyrosinase activity in a concentration-dependent manner.

Keywords

References

  1. Ando H, Kondoh H, Ichihashi M, Hearing VJ. 2007. Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase. J. Invest. Dermatol. 127: 751-761. https://doi.org/10.1038/sj.jid.5700683
  2. Balboa EM, Conde E, Moure A, Falque E, Dominguez H. 2013. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem. 138: 1764-1785. https://doi.org/10.1016/j.foodchem.2012.11.026
  3. Cha SH, Ko SC, Kim D, Jeon YJ. 2011. Screening of marine algae for potential tyrosinase inhibitor: those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish. J. Dermatol. 38: 354-363. https://doi.org/10.1111/j.1346-8138.2010.00983.x
  4. Choi TY, Kim JH, Ko DH, Kim CH, Hwang JS, Ahn S, et al. 2007. Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds. Pigment Cell Res. 20: 120-127. https://doi.org/10.1111/j.1600-0749.2007.00365.x
  5. Eisen JS. 1996. Zebrafish make a big splash. Cell 87: 969-977. https://doi.org/10.1016/S0092-8674(00)81792-4
  6. Fishman MC. 1999. Zebrafish genetics: the enigma of arrival. Proc. Natl. Acad. Sci. USA 96: 10554-10556. https://doi.org/10.1073/pnas.96.19.10554
  7. Jones K, Hughes J, Hong M, Jia Q, Orndorff S. 2002. Modulation of melanogenesis by aloesin: a competitive inhibitor of tyrosinase. Pigment Cell Res. 15: 335-340. https://doi.org/10.1034/j.1600-0749.2002.02014.x
  8. Kim JA, Lee JM, Shin DB, Lee NH. 2004. The antioxidant activity and tyrosinase inhibitory activity of phlorotannins in Ecklonia cava. Food Sci. Biotechnol. 13: 476-480.
  9. Kim KN, Yang HM, Kang SM, Kim D, Ahn G, Jeon YJ. 2013. Octaphlorethol A isolated from Ishige foliacea inhibits alpha-MSH-stimulated induced melanogenesis via ERK pathway in B16F10 melanoma cells. Food Chem. Toxicol. 59: 521-526. https://doi.org/10.1016/j.fct.2013.06.031
  10. Kim YJ, Kang KS, Yokozawa T. 2008. The anti-melanogenic effect of pycnogenol by its anti-oxidative actions. Food Chem. Toxicol. 46: 2466-2471. https://doi.org/10.1016/j.fct.2008.04.002
  11. Lee SH, Kang SM, Ko SC, Kang MC, Jeon YJ. 2013. Octaphlorethol A, a novel phenolic compound isolated from Ishige foliacea, protects against streptozotocin-induced pancreatic beta cell damage by reducing oxidative stress and apoptosis. Food Chem. Toxicol. 59: 643-649. https://doi.org/10.1016/j.fct.2013.07.011
  12. Lee SH, Kang SM, Ko SC, Lee DH, Jeon YJ. 2012. Octaphlorethol A, a novel phenolic compound isolated from a brown alga, Ishige foliacea, increases glucose transporter 4-mediated glucose uptake in skeletal muscle cells. Biochem. Biophys. Res. Commun. 420: 576-581. https://doi.org/10.1016/j.bbrc.2012.03.036
  13. Manzoor Z, Mathema VB, Chae D, Kang HK, Yoo ES, Jeon YJ, Koh YS. 2013. Octaphlorethol A inhibits the CpG-induced inflammatory response by attenuating the mitogen-activated protein kinase and NF-kappaB pathways. Biosci. Biotechnol. Biochem. 77: 1970-1972. https://doi.org/10.1271/bbb.130299
  14. Park DC, Ji CI, Kim SH, Jung KJ, Lee TG, Kim IS, et al. 2000. Characteristics of tyrosinase inhibitory extract from Ecklonia stolonifera. J. Fish Sci. Tech. 3: 195-199.
  15. Roh JS, Han JY, Kim JH, Hwang JK. 2004. Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis. Biol. Pharm. Bull. 27: 1976-1978. https://doi.org/10.1248/bpb.27.1976
  16. Yang HM, Ham YM, Yoon WJ, Roh SW, Jeon YJ, Oda T, et al. 2012. Quercitrin protects against ultraviolet B-induced cell death in vitro and in an in vivo zebrafish model. J. Photochem. Photobiol. B 114: 126-131. https://doi.org/10.1016/j.jphotobiol.2012.05.020
  17. Zawar VP, Mhaskar ST. 2004. Exogenous ochronosis following hydroquinone for melasma. J. Cosmet. Dermatol. 3: 234-236. https://doi.org/10.1111/j.1473-2130.2004.00089.x

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