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우리나라 야생화에서 분리한 효모의 항산화 활성과 Xanthine Oxidase 및 Tyrosinase 저해활성

Antioxidant Activity and Inhibitory Activities of Xanthine Oxidase and Tyrosinase of Yeasts from Wild Flowers in Korea

  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 김나미 (한국인삼공사 글로벌제품연구소) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology Paichai University) ;
  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology Paichai University) ;
  • Kim, Na-Mi (Global Products Research Institute, Korea Ginseng Corp.) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology Paichai University)
  • 투고 : 2015.06.09
  • 심사 : 2015.06.17
  • 발행 : 2015.06.30

초록

제주도, 울릉도, 욕지도 등 우리나라 섬들과 계족산, 오서산, 백암산 등의 산에서 서식하는 야생화들에서 분리한 207 효모 균주들을 YPD 배지에 24시간 배양하여 각각의 무세포추출물과 배양상등액을 제조한 후 이들의 항산화활성과 Xanthine oxidase 저해활성 및 tyrosinase 저해활성을 측정하였다. 섬 지역 야생화들에서 분리한 Kuraishia capsulata UL40-2와 산 지역 야생화에서 분리한 Sporobolomyces ruberrimus 121-Z-3의 무세포추출물이 각각 46.4%와 48.3%의 비교적 높은 항통풍성 xanthine oxidase 저해활성을 보였다. 또한 Starmerella bombicola 80-J-1의 무세포추출물 역시 36.2%의 미백성 tyrosinase 저해활성을 보였다. 그 밖의 항산화활성과 tyrosinase 저해활성은 모든 분리효모들에서 없거나 30% 미만으로 미약하였다. 이들 가운데 xanthine oxidase 저해활성이 우수하였던 Kuraishia capsulate UL40-2와 Sporobolomyces ruberrimus 121-Z-3의 xanthine oxidase 저해제의 최적 생산 조건을 검토한 결과 두 효모 각각을 YPD에 접종하여 $30^{\circ}C$에서 24시간 배양하여 얻은 무세포추출물이 47.2%와 49.5%의 높은 xanthine oxidase 저해활성을 보였다.

We prepared supernatants and cell-free extracts of yeasts isolated from wild flowers in Korea, and their antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase were investigated. Among them, cell-free extracts of Kuraishia capsulata UL40-2 and Sporobolomyces ruberrimus 121-Z-3 showed significantly high xanthine oxidase inhibitory activity of 46.4% and 48.3%, respectively. Starmerella bombicola 80-J-1 also showed tyrosinase inhibitory activity of 36.2% in the cell-free extract. Other antioxidant activities and tyrosinase inhibitory activities were not detected or were below 20%. Maximal production of the xanthine oxidase inhibitors were observed when Kuraishia capsulate UL40-2 and Sporobolomyces ruberrimus 121-Z-3 were cultured in the yeast extract-peptone-dextrose media at $30^{\circ}C$ for 24 hour, respectively.

키워드

참고문헌

  1. Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from traditional Meju. Kor J Food Nutr 1999;12:490-5.
  2. Jeong SC, Kim JH, Kim NM, Lee JS. Production of antihypertensive angiotensin I-converting enzyme inhibitor from Malassezia pachydermatis G-14. Mycobiology 2005;33:142-6. https://doi.org/10.4489/MYCO.2005.33.3.142
  3. Kim JH, Lee DH, Jeong SC, Chung KS, Lee JS. Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae. J Microbiol Biotechnol 2004;14:1318-23.
  4. Lee DH, Lee DH, Lee JS. Characterization of a new antidementia ${\beta}$-secretase inhibitory peptide from Saccharomyces cerevisiae. Enzyme Microb Technol 2007;42:83-8. https://doi.org/10.1016/j.enzmictec.2007.08.003
  5. Lee DH, Lee JS, Yi SH, Lee JS. Production of the acetylcholinesterase inhibitor from Yarrowia lipolytica S-3. Mycobiology 2008;36:102-5. https://doi.org/10.4489/MYCO.2008.36.2.102
  6. Jeong SC, Lee DH, Lee JS. Production and characterization of an anti-angiogenic agent from Saccharomyces cerevisiae K-7. J Micriobiol Biotechnol 2006;16:1904-11.
  7. Jang IT, Kim YH, Yi SH, Lim SI, Lee JS. Screening of a new fibrinolytic substances-producing yeast. Kor J Mycol 2011;39:227-8. https://doi.org/10.4489/KJM.2010.39.3.227
  8. Jang IT, Kim YH, Kang MG, Yi SH, Lim SI, Lee JS. Production of tyrosinase inhibitor from Saccharomyces cerevisiae. Kor J Mycol 2012;40:60-4. https://doi.org/10.4489/KJM.2012.40.1.060
  9. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  10. Min JH, Ryu JJ, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Beakamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  11. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  12. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  13. Hyun SH, Han SM, Lee JS. Characteristics and physiological functionalities of unrecorded yeasts from wild flowers of Seonyudo in Jeollabukdo, Korea. Kor J Microbiol Biotechnol 2014;42:402-6. https://doi.org/10.4014/kjmb.1409.09001
  14. Kim HK, Lee EN, Geum JW, Lee JS. Nutritional characteristics and physiological functionality of polyplant extracts from some vegetables and medicinal plants. Kor J Food Nutr 2008;21:410-5.
  15. Hyun SH, Lee JS. Microbiological characteristics and physiological functionality of new records of yeasts from wild flowers in Yokjido, Korea. Mycobiology 2014;42:198-202. https://doi.org/10.5941/MYCO.2014.42.2.198
  16. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  17. Jang IT, Hyun SH, Shin JW, Lee YH, Ji JH, Lee JS. Characterization of an anti-gout xanthine oxidase inhibitor from Pleurotus ostreatus. Mycobiology 2014;42:296-300. https://doi.org/10.5941/MYCO.2014.42.3.296
  18. Hyun SH. Isolation and identification of yeasts from wild flowers in several islands and mountains of Korea and determination of their functionality [dissertation]. Daejeon (Korea): University of Paichai; 2015.