Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Effect of Banhahoobak-tang (Banxiahoupo-tang) Extract (BHTe) on Psychological Stress

반하후박탕(半夏厚朴湯)이 생쥐의 심리적 스트레스에 미치는 영향과 유전자 분석

  • Choi, Geum-Ae (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongshin University) ;
  • Cho, Su-In (Department of School of Korean Medicine, Pusan National University) ;
  • Kim, Kyeong-Su (Department of College of Oriental Medicine, Dongshin University) ;
  • Choi, Chang-Won (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongshin University) ;
  • Wei, Tung-Shuen (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Yang, Seung-Joung (Department of Oriental Gynecology, College of Oriental Medicine, Dongshin University) ;
  • Park, Soo-Yeon (Department of Oriental Opthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongshin University) ;
  • Kim, Kyeong-Ok (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Dongshin University)
  • 최금애 (동신대학교 한의과대학 한방신경정신과학교실) ;
  • 조수인 (부산대학교 한의학전문대학원) ;
  • 김경수 (동신대학교 한의과대학) ;
  • 최창원 (동신대학교 한의과대학 한방내과학교실) ;
  • 위통순 (동신대학교 한의과대학 침구학교실) ;
  • 양승정 (동신대학교 한의과대학 한방부인과학교실) ;
  • 박수연 (동신대학교 한의과대학 한방안이비인후피부과학교실) ;
  • 김경옥 (동신대학교 한의과대학 한방신경정신과학교실)
  • Received : 2015.05.23
  • Accepted : 2015.06.08
  • Published : 2015.06.30
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Abstract

Objectives: Banhahoobak-tang has been used to treat plum-pit qi, chest and hypochondriac distension, moist or greasy tongue coat, and wiry slow or wiry slippery pulse. It might be used to control coughing and vomiting. We observed that Banhahoobak-tang extract (BHTe) had anti-psychological stress effect. The objective of this study was to determine the effect of BHTe on restoring the transcriptional regulation of genes related to psychological stress. Methods: After giving psychological stress to mice, BHTe was orally administered at 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, the gene expression changes were determined by microarray. Transcription factor binding site (TFBS) analysis showed up- and down-regulated genes related to psychological stress were protected by BHTe and segregated according to the structure of TFBS. We performed text based Pubmed search to select significant target genes involved in psychological stress affected by BHTe. Results: 1. Serum corticosterone level was decreased in the BHTe administered group, although the psychological stress was increased. 2. The BHTe administered group had no significant change in noradrenaline content in brain tissue, but the psychological stress group had decreased level. 3. The BHTe administered group had increased time of staying at open-arm than the psychological stress group. 4. Microarray revealed that TANK and RARA genes were up-regulated genes while AES, CDC42, FOS, NCL, and PVR were down-regulated genes by psychological stress but restored by BHTe.

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