Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Characteristics of Acidovorax citrulli Population Causing Bacterial Fruit Blotch against Cucurbits in Korea

국내 박과 작물에서 과실썩음병을 일으키는 Acidovorax citrulli 집단의 유전적 특성

  • Song, Jeong Young (Department of Applied Biology, Chungnam National University) ;
  • Park, Su Yeon (Department of Applied Biology, Chungnam National University) ;
  • Seo, Mun Won (Department of Applied Biology, Chungnam National University) ;
  • Nam, Myeong Hyeon (Nonsan Strawberry Experiment Station, Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lim, Hyoun Sub (Department of Applied Biology, Chungnam National University) ;
  • Lee, Seong-Chan (Protected Horticulture Research Institute, National Institute of Horticultural and Herbal Science) ;
  • Lee, Youn Su (Division of Bio-resource Sciences, Kangwon National University) ;
  • Kim, Hong Gi (Department of Applied Biology, Chungnam National University)
  • 송정영 (충남대학교 응용생물학과) ;
  • 박수연 (충남대학교 응용생물학과) ;
  • 서문원 (충남대학교 응용생물학과) ;
  • 남명현 (충남농업기술원 논산딸기시험장) ;
  • 임현섭 (충남대학교 응용생물학과) ;
  • 이성찬 (국립원예특작과학원 시설원예연구소) ;
  • 이윤수 (강원대학교 생물자원과학부) ;
  • 김홍기 (충남대학교 응용생물학과)
  • Received : 2015.02.23
  • Accepted : 2015.06.08
  • Published : 2015.06.30
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Abstract

Acidovorax citrulli, the causal agent of bacterial fruit blotch, has caused an economically destructive damage in cucurbits cultivation fields worldwide. To consider more effective disease management, 33 A. citrulli isolates collected from various cucurbits in Korea were analysed by multi-locus phylogeny using five conserved loci(16S rRNA, adk, gltA, glyA, pilT). Two distinct groups (KCC1 and KCC2) in the population were identified on the base of group-specific genetic variation. Out of them, the predominant group was KCC2 and both groups included isolates from melon, cucumber and watermelon. Sixty-four percent of KCC1 isolates were recovered from non-watermelon hosts and seventy-two percent of KCC2 isolates from watermelon. This study presented that there was genetic differentiation among A. citrulli population in Korea. Also, these results will be applied as a very useful data in effective disease management.

Acidovorax citrulli는 전 세계적으로 다양한 박과 작물 재배포장에서 과실썩음병(bacterial fruit blotch)을 일으키며, 경제적으로 큰 피해를 주고 있다. 효과적인 병 방제 전략을 수립하기 위한 기초자료를 얻고자 전국의 주요 박과 작물 재배지역으로부터 얻은 A. citrulli 33균주들을 대상으로 5개 유전자(16S rRNA, adk, gltA, glyA, pilT)를 이용한 multi-locus 유연관계 분석을 실시하였다. 국내 A. citrulli 집단은 종 특이적이며, 그룹 특이적인 유전적 특성들을 기반으로 KCC1과 KCC2 그룹으로 나누어졌으며, 이들은 수박과 오이와 멜론분리균들을 모두 포함했고 이들 중 KCC2 그룹 균주들의 발생이 우세했다. KCC1 그룹 균주들은 수박이외 기주에서의 발생비율이 64%였으나, KCC2 그룹에서는 수박분리균의 비율이 72%로 우세하였다. 본 연구결과는 국내 A. citrulli 집단에 유전적인 변이가 존재한다는 것을 밝혔으며, 효과적인 박과 작물 과실썩음병 방제체계 수립에 있어서 매우 유용한 자료로 활용될 것으로 판단된다.

Keywords

References

  1. Assis, S. M. P., Mariano, R. L. R., Silva-Hanlin, D. M. W. and Duarte, V. 1999. Bacterial fruit blotch caused by Acidovorax avenae subsp. citrulli in melon, in the state of Rio Grande Do Norte, Brazil. Fitopatol. Bras. 24: 191.
  2. Burdman, S., Kots, N., Kritzman, G. and Kopelowitz, J. 2005. Molecular, physiological, and host-range characterization of Acidovorax avenae subsp. citrulli isolates from watermelon and melon in Israel. Plant Dis. 89: 1339-1347. https://doi.org/10.1094/PD-89-1339
  3. Burdman, S. and Walcott, R. R. 2012. Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry. Mol. Plant Pathol. 13: 805-815. https://doi.org/10.1111/j.1364-3703.2012.00810.x
  4. Cheng, A. H., Hsu, Y. L., Huang, T. C. and Wang, H. L. 2000. Susceptibility of cucurbits to Acidovorax avenae subsp. citrulli and control of fruit blotch on melon. Plant Pathol. Bull. 9: 151-156.
  5. Dutta, B., Scherm, H., Gitaitis, R. D. and Walcott, R. R. 2012. Acidovorax citrulli seed inoculum load affects seedling transmission and spread of bacterial fruit blotch of watermelon under greenhouse conditions. Plant Dis. 96: 705-711. https://doi.org/10.1094/PDIS-04-11-0292
  6. Feng, J. J., Schuenzel, E. L., Li, J. and Schaad, N. W. 2009. Multilocus sequence typing reveals two evolutionary lineages of Acidovorax avenae subsp. citrulli. Phytopathology 99: 913-920. https://doi.org/10.1094/PHYTO-99-8-0913
  7. Gemir, G. 1996. A new bacterial disease of watermelon in Turkey: bacterial fruit blotch of watermelon [Acidovorax avene subsp. citrulli (Schaad et al. ) Willems et al.]. J. Turk. Phytopathol. 25: 43-49.
  8. Hopkins, D. L. 1991. Control of bacterial fruit blotch of watermelon with cupric hydroxide. Phytopathology 81: 1228.
  9. Hopkins, D. L. 1989. Bacterial fruit blotch of watermelon: a new disease in the eastern USA. Proc. Cucurbitaceae 89: 74-75.
  10. Hopkins, D. L. and Thompson, C. M. 2002a: Evaluation of Citrullus sp. germplasm for resistance to Acidovorax avenae subsp. citrulli. Plant Dis. 86: 61-64. https://doi.org/10.1094/PDIS.2002.86.1.61
  11. Hopkins, D. L. and Thompson, C. M. 2002b: Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits. Hortscience 37: 924-926.
  12. Isakeit, T., Black, M. C., Barnes, L. W. and Jones, J. B. 1997. First report of infection of honeydew with Acidovorax avenae subsp. citrulli. Plant Dis. 81: 694.
  13. Martin, H. L. and Horloc, C. M. 2002: First report of Acidovorax avenae subsp. citrulli as a pathogen of Gramma in Australia. Plant Dis. 86: 1406.
  14. Makizumi, Y., Igarashi, M., Gotoh, K., Murao, K., Yamamoto, M., Udonsri, N., Ochiai, H., Thummabenjapone, P. and Kaku, H. 2011. Genetic diversity and pathogenicity of cucurbit-associated Acidovorax. J. Gen. Plant Pathol. 77: 24-32. https://doi.org/10.1007/s10327-010-0273-y
  15. Rane, K. K. and Latin, R. X. 1992. Bacterial fruit blotch of watermelon: Association of the pathogen with seed. Plant Dis. 76: 509-512. https://doi.org/10.1094/PD-76-0509
  16. Schaad, N. W., Postnikova, E. and Randhawa, P. 2003. Emergence of Acidovorax avenae subsp. citrulli as a crop threatening disease of watermelon and melon. In: Pseudomonas syringae and Related Pathogens, ed. by N. S. Lacobellis, pp. 573-581. Kluwer Academic Publishers, Dordrecht, The Netherlands.
  17. Schaad, N. W., Postnikova, E., Sechler, A., Claflin, L. E., Vidaver, A. K., Jones, J. B., Agarkova, I., Ignatov, A., Dickstein, E. and Ramundo, B. A. 2008. Reclassification of subspecies of Acidovorax avenae as A. avenae (Manns, 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli (Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov. Syst. Appl. Microbiol. 31: 434-446. https://doi.org/10.1016/j.syapm.2008.09.003
  18. Seo, S. T., Park, J. H., Lee, J. S., Han, K. S. and Cheong, S. R. 2006. Bacterial fruit blotch of melon caused by Acidovorax avenae subsp. citrulli. Res. Plant Dis. 12: 185-188. (In Korean) https://doi.org/10.5423/RPD.2006.12.3.185
  19. Song, W. Y., Kim, H. M., So, I. Y. and Kang, Y. K. 1991. Pseudomonas pseudoalcaligenes subsp. citrulli: The causal agent of bacterial fruit blotch rot on watermelon. Korean J. Plant Pathol. 7: 177-182. (In Korean)
  20. Shirakawa, T., Kikuchi, S., Kato, T., Abiko, A. and Kaiwa, A. 2000. Occurrence of watermelon bacterial fruit blotch in Japan. Jpn. J. Plant Pathol. 66: 223-231.
  21. Walcott, R. R., Langston, D, B., Sanders, F. H. and Gitaitis, R. D. 2000. Investigating intraspecific variation of Acidovorax avenae subsp. citrulli using DNA fingerprinting and whole cell fatty acid analysis. Phytopathology 90: 191-196. https://doi.org/10.1094/PHYTO.2000.90.2.191
  22. Walcott, R. R., Gitaitis, R. D. and Castro, A. C. 2003. Role of blossoms in watermelon seed infestation by Acidovorax avenae subsp. citrulli. Phytopathology 93: 528-534. https://doi.org/10.1094/PHYTO.2003.93.5.528
  23. Walcott, R. R., Fessehaie, A. and Castro, A. C. 2004. Differences in pathogenicity between two genetically distinct groups of Acidovorax avenae subsp. citrulli on cucurbit hosts. J. Phytopathol. 152: 277-285. https://doi.org/10.1111/j.1439-0434.2004.00841.x
  24. Walcott, R. R. 2008. Integrated pest management of bacterial fruit blotch of cucurbits. In: Integrated Management of Diseases Caused by Fungi, Phytoplasma and Bacteria, eds. by A. Ciancio and K. G. Mukerji, pp. 191-209. Springer Netherlands.
  25. Wechter, W. P., Levi, A., Ling, K.-S., Kousik, C. and Block, C. C. 2011. Identification of resistance to Acidovorax avenae subsp. citrulli among melon (Cucumis spp.) plant introductions. HortScience 46: 207-212.
  26. Yan, S., Yang, Y., Wang, T., Zhao, T. and Schaad, N. W. 2013. Genetic diversity analysis of Acidovorax citrulli in China. Eur. J. Plant Pathol. 136: 171-181. https://doi.org/10.1007/s10658-012-0152-9

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