Biomaterials and Biomechanics in Bioengineering

Techno-Press (테크노프레스)
권호 표지
DOI QR코드

DOI QR Code

Dental Pulp Stem Cell: A review of factors that influence the therapeutic potential of stem cell isolates

  • Young, Aubrey (Orthodontics and Dentofacial Orthopedics, UNLV School of Dental Medicine) ;
  • Kingsley, Karl (Biomedical Science, Director of Student Research, UNLV School of Dental Medicine)
  • Received : 2014.09.22
  • Accepted : 2015.03.26
  • Published : 2015.06.25
⟨ Previous Next ⟩

Abstract

Undifferentiated stem cells are being studied to obtain information on the therapeutic potential of isolates that are produced. Dental Pulp Stem Ccell (DPSC) may provide an abundant supply of highly proliferative, multipotent Mesenchymal Stem Cells (MSC), which are now known to be capable of regenerating a variety of human tissues including bone and other dental structures. Many factors influence DPSC quality and quantity, including the specific methods used to isolate, collect, concentrate, and store these isolates once they are removed. Ancillary factors, such as the choice of media, the selection of early versus late passage cells, and cryopreservation techniques may also influence the differentiation potential and proliferative capacity of DPSC isolates. This literature review concludes that due to the delicate nature of DPSC, more research is needed for dental researchers and clinicians to more fully explore the feasibility and potential for isolating and culturing DPSCs extracted from adult human teeth in order to provide more accurate and informed advice for this newly developing field of regenerative medicine.

Keywords

References

  1. Alleman, M., Low, E., Truong, K., Huang, E., Hill, C.K., Chen, T.Y., Deaton, M. and Kingsley, K. (2013), "Dental pulp-derived stem cells (DPSC) differentiation in vitro into odontoblast and neural progenitors during cell passaging is associated with alterations in cell survival and viability", Int. J. Med. Biomed. Res., 2(2), 133-141. https://doi.org/10.14194/ijmbr.226
  2. Bluteau, G., Luder, H.U., De Bari, C. and Mitsiadis, T.A. (2008), "Stem cells for tooth engineering", Eur. Cell. Mater., 16(1), 1-9. https://doi.org/10.22203/eCM.v016a01
  3. Coppe, C., Zhang, Y. and Den, Besten, P.K. (2009), "Characterization of primary dental pulp cells in vitro", Pediatr. Dent, 31(7), 467-471.
  4. Daltoe, F.P., Mendonca, P.P., Mantesso, A. and Deboni, M.C. (2014), "Can SHED or DPSCs be used to repair/regenerate non-dental tissues? A systematic review of in vivo studies", Braz. Oral. Res., 28(1), 1-7.
  5. Davies, O.G., Smith, A.J., Cooper, P.R., Shelton, R.M. and Scheven, B.A. (2014), "The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues", Cryobiol., 69(2), 342-347. https://doi.org/10.1016/j.cryobiol.2014.08.003
  6. De Soza, Priscilla, Vianna et al. (2013), "Human immatrue dental pulp stem cells, their application to cell therapy and bioengineering: An analysis by systematic revision of the last decade of literature", Anatom. Rec.
  7. Dziubinska, P., Jaskolska, M., Przyborowska, P. and Adamiak, Z. (2013), "Stem cells in dentistry-review of literature", Pol. J. Vet. Sci., 16(1), 135-140.
  8. Ennis, W.J., Sui, A. and Bartholomew, A. (2013), "Stem cells and healing: Impact on inflammation", Adv. Wound Care (New Rochelle), 2(7), 369-378. https://doi.org/10.1089/wound.2013.0449
  9. Ferro, F., Spelat, R. and Baheney, C.S. (2014), "Dental Pulp Stem Cell (DPSC) isolation, characterization, and differentiation", Meth. Mol. Biol., 1210, 91-115. https://doi.org/10.1007/978-1-4939-1435-7_8
  10. Ferro, F., Spelat, R., Beltrami, A.P., Cesselli, D. and Curcio, F. (2012), "Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum", PLoS One, 7(11), e48945. https://doi.org/10.1371/journal.pone.0048945
  11. Govindasamy, V., Ronald, V.S., Totey, S., Din, S.B., Mustafa, W.M., Totey, S., Zakaria, Z. and Bhonde, R.R. (2010), "Micromanipulation of culture niche permits long-term expansion of dental pulp stem cellsan economic and commercial angle", Vitro Cell Dev. Biol. Anim., 46(9), 764-773. https://doi.org/10.1007/s11626-010-9332-0
  12. Hirata, T.M., Ishkitiev, N., Yaegaki, K., Calenic, B., Ishikawa, H., Nakahara, T., Mitev, V., Tanaka, T. and Haapasalo, M. (2010), "Expression of multiple stem cell markers in dental pulp cells cultured in serumfree media", J. Endod., 36(7), 1139-1144. https://doi.org/10.1016/j.joen.2010.03.002
  13. Huang, G.T., Sonoyama, W., Chen, J. and Park, S.H. (2006), "In vitro characterization of human dental pulp cells: various isolation methods and culturing environments", Cell Tissue Res., 324(2), 225-236. https://doi.org/10.1007/s00441-005-0117-9
  14. Huang, G.T., Sonoyama, W., Liu Y., Liu H., Wang S. and Shi S. (2008), "The hidden treasure in apical papilla: The potential role in pulp/dentin regeneration and bioroot engineering", J. Endod., 34, 645-651. https://doi.org/10.1016/j.joen.2008.03.001
  15. Huang, G.T., Gronthos, S. and Shi, S. (2009), "Mesenchymal stem cells derived from dental tissues vs. those from other sources; their biology and role in regenerative medicine", J. Dent. Res., 88(9), 792-806. https://doi.org/10.1177/0022034509340867
  16. Hung, E., Lee, S., Fitzgerald, B., Hill, C.K. and Kingsley, K. (2013), "Dental pulp-derived stem cell (DPSC) survival and viability may correlate with specific patient demographics", FDSRI Winter, 1(3), 14-21.
  17. Iohara, K., Zheng, L., Ito, M., Tomokiyo, A., Matsushita, K. and Nakashima, M. (2006), "Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentiongenesis, chondrogenesis, adipogenesis, and neurogenesis", Stem Cell., 24(11), 2493-2503. https://doi.org/10.1634/stemcells.2006-0161
  18. Ikeda, E., Yagi, K., Kojima, M., Yagyuu, T., Ohshima, A., Sobajima, S., Tadokoro, M., Katsube, Y., Isoda, K., Kondoh, M., Kawase, M., Go, J.J., Adachi, H., Yokota, Y., Kirita, T. and Ohgushi, H. (2008), "Multipotent cells from the human third molar: Feasibility of cell-based therapy for liver disease", Differentiation, 76(5), 495-505. https://doi.org/10.1111/j.1432-0436.2007.00245.x
  19. Jeon, M., Song, J.S., Choi, B.J., Choi, H.J., Shin, D.M., Jung, H.S. and Kim, S.O. (2014), "In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth", Arch Oral. Biol., 59(10), 1013-1023. https://doi.org/10.1016/j.archoralbio.2014.06.002
  20. Jones, K.B. and Klein, O.D. (2013), "Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey", Int. J. Oral. Sci., 5(3), 121-129. https://doi.org/10.1038/ijos.2013.46
  21. Karamzadeh, R., Eslaminejad, M.B. and Aflatoonian, R. (2012), "Isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different methods", J. Vis. Exp., 24(69), e4372.
  22. Kellner, M., Steindorff, M.M., Strempel, J.F., Winkel, A., Kuhnel, M.P. and Stiesch, M. (2014), "Differences of isolated dental stem cells dependent on donor age and consequences for autologous tooth replacement", Arch Oral. Biol., 59(6), 559-567. https://doi.org/10.1016/j.archoralbio.2014.02.014
  23. King, N.M. and Perrin, J. (2014), "Ethical issues in stem cell research and therapy", Stem Cell. Res. Ther., 5(4), 85. https://doi.org/10.1186/scrt474
  24. Kolios, G. and Moodley, Y. (2012), "Introduction to stem cells and regenerative medicine", Respiration, 85(1), 3-10. https://doi.org/10.1159/000345615
  25. Kramer, P.R., Nares, S., Kramer, S.F., Grogan, D. and Kaiser, M. (2006), "Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro", J. Dent. Res., 83(1), 27-34. https://doi.org/10.1177/154405910408300106
  26. Lee, W., Wong, R.W., Ikegami, T. and Hagg, U. (2008), "Maxillary second molar extractions in orthodontic treatment", World J. Orthod., 9(1), 52-61.
  27. Langer, R. and Vacanti, J.P. (2005), "Tissue engineering", Sci., 260, 920-926.
  28. Li, Z., Jiang, C.M., An, S., Cheng, Q., Huang, Y.F., Wand, Y.T., Gou, Y.C., Xiao, L., Yu, W.J. and Wang, J. (2013), "Immunomodulartory properties of dental tissue-derived mesenchymal stem cells", Oral Diseases, doi:10.1111.
  29. Liao, H.T. and Chen, C.T. (2014), "Osteogenic potential: Comparison between bone marrow and adiposederived mesenchymal stem cells", World J. Stem Cell., 6(3), 288-295. https://doi.org/10.4252/wjsc.v6.i3.288
  30. Lin, N.H., Gronthos, S. and Bartold, M. (2009, 2000), "Stem cells and future periodontal regeneration", Periodontol., 51(1), 239-251.
  31. Lindemann, D., Werle, S.B., Steffens, D., Garcia-Godoy, F., Pranke, P. and Casagrande, L. (2014), "Effects of cryopreservation on the characteristics of dental pulp stem cells of intact deciduous teeth", Arch Oral Biol., 59(9), 970-976. https://doi.org/10.1016/j.archoralbio.2014.04.008
  32. Loveland, K., Young, A., Khadiv, M., Culpepper, M. and Kingsley, K. (2014), "Dental Pulp Stem Cell (DPSC) pluripotency enhanced by Transforming Growth Factor (TGF-${\beta}$1) in Vitro may be inhibited by differentiation-inducing factors laminin-5 and dexamethasone", Int. J. Biol. Sci. Appl., 1(3), 55-61.
  33. Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L.W., Robey, P.G. and Shi, S. (2003), "Stem cells from human exfoliated deciduous teeth", Proceedings of the National Academy of Sciences of the United States of America, 100(10), 5807-5812. https://doi.org/10.1073/pnas.0937635100
  34. Morsczeck, C., Gotz, W., Scierholz, J., Zeihofer, F., Kuhn, U., Mohl, C., Sippel, C. and Hoffmann, K.H. (2005), "Isolation of precursor cells from human dental follicle of wisdom teeth", Matrix Biol., 24(2), 155-165. https://doi.org/10.1016/j.matbio.2004.12.004
  35. Peng, L., Ye, L. and Zhu, X.D. (2009), "Mesenchymal stem cells and tooh engineering", Int. J. Oral Sci., 1(1), 6-12. https://doi.org/10.4248/ijos.08032
  36. Perry, B.C., Zhou, D., Wu, X., Yang, F.C., Byers, M.A., Chu, T.M., Hockema, J.J., Woods, E.J. and Goebel, W.S. (2008), "Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use", Tissue Eng. Part C Meth., 14(2), 149-156. https://doi.org/10.1089/ten.tec.2008.0031
  37. Ponnaiyan, D. (2014), "Do dental stem cells depict distinct characteristics?-Establishing their "phenotypic fingerprint" ", Dent. Res. J. (Isfahan), 11(2), 163-172.
  38. Raveh-Amit, H., Berzsenyi, S., Vas, V., Ye, D. and Dinnyes, A. (2013), "Tissue resident stem cells: till death do us part", Biogerontol., 14(6), 573-590. https://doi.org/10.1007/s10522-013-9469-9
  39. Rai, S., Kaur, M. and Kaur, S. (2013). "Applications of stem cells in interdisciplinary dentistry and beyond: An overview", Annal. Med. Hlth. Sci. Res., 3(2), 245-254. https://doi.org/10.4103/2141-9248.113670
  40. Rinaldi, L. and Benitah, S.A. (2014), "Epigenetic regulation of adult stem cell function", FEBS J., 282(9), 1589-1604. https://doi.org/10.1111/febs.12946
  41. Rasmusson, I. (2006), "Immune modulation by mesenchymal stsem cells", Exp. Cell Res., 312(12), 2169-2179. https://doi.org/10.1016/j.yexcr.2006.03.019
  42. Sedgley, C.M. and Botero, T.M. (2012), "Dental stem cells and their source", Dent. Clin. North Am., 56(3), 549-561. https://doi.org/10.1016/j.cden.2012.05.004
  43. Seo, B.M., Miura, M., Gronthos, S., Bartold, P.M., Batouli, S., Brahim, J., Young, M., Robey, P.G., Wang, C.Y. and Shi, S. (2004), "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364(9429), 149-155. https://doi.org/10.1016/S0140-6736(04)16627-0
  44. Shilpa, P.S., Kaul, R., Sultana, N. and Bhat, S. (2013), "Stem cells: Boon to dentistry and medicine", Dent. Res. J. (Isfahan), 10(2), 149-154. https://doi.org/10.4103/1735-3327.113321
  45. Sonoyama, W., Liu, Y., Fang, D., Yamaza, T., Seo, B.M., Zhang, C., Liu, H., Gronthos, S., Wang, C.Y., Wang, S. and Shi, S. (2006), "Mesenchymal stem cell-mediated functional tooth regeneration in swine", PLoS One, 1(1), e79. https://doi.org/10.1371/journal.pone.0000079
  46. Sonoyama, W., Liu, Y., Yamaza, T., Tuan, R.S., Wang, S., Shi, S. and Huang, G.T. (2008), "Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study", J. Endo., 34(2), 166-171. https://doi.org/10.1016/j.joen.2007.11.021
  47. Suchanek, J., Soukup, T., Visek, B., Ivancakova, R., Kucerova, L. and Mokry, J. (2009), "Dental pulp stem cells and their characterization", Biomed. Paper., 153(1), 31-35. https://doi.org/10.5507/bp.2009.005
  48. Tandon, S., Saha, R., Rajendran, R. and Nayak, R. (2010), "Dental pulp stem cells from primary and permanent teeth: quality analysis", J. Clin. Pediatr. Dent., 35(1), 53-58. https://doi.org/10.17796/jcpd.35.1.e33x0135r7670287
  49. Tatullo, M., Marrelli, M., Shakesheff, K.M. and White, L.J. (2014), "Dental pulp stem cells: function, isolation and applications in regenerative medicine", J. Tissue Eng. Regen. Med., doi: 10.1002/term.1899.
  50. Tirino, V., Paino, F., d'Aquino, R., Desiderio, V., De Rosa, A. and Papaccio, G. (2011), "Methods for the identification, characterization and banking of human DPSCs: current strategies and perspectives", Stem Cell. Rev., 7(3), 608-615. https://doi.org/10.1007/s12015-011-9235-9
  51. Uccelli, A., Moretta, L. and Pistoia, V. (2006), "Immunoregulatory function of mesenchymal stem cells", Eur. J. Immunol., 36, 2566-2573. https://doi.org/10.1002/eji.200636416
  52. Verma, K., Bains, R., Bains, V.K., Rawtiya, M., Loomba, K. and Srivastava, S.C. (2014), "Therapeutic potential of dental pulp stem cells in regenerative medicine: An overview", Dent. Res. J. (Isfahan), 11(3), 302-308.
  53. Viale-Bouroncle, S., Gosau, M. and Morsczeck, C. (2014), "Laminin regulates the osteogenic differentiation of dental follicle cells via integrin-${\alpha}$2/-${\beta}$1 and the activation of the FAK/ERK signaling pathway", Cell Tissue Res., 357(1), 345-354. https://doi.org/10.1007/s00441-014-1869-x
  54. Vishwanath, V.R., Nadig, R.R., Nadig, R., Prasanna, J.S., Karthik, J. and Pai, V.S. (2013), "Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth: An in vitro study", J. Conserv. Dent., 16(5), 423-428. https://doi.org/10.4103/0972-0707.117509
  55. Woods, E.J., Perry, B.C., Hockema, J.J., Larson, L., Zhou, D. and Goebel, W.S. (2009), "Optimized cryopreservation method for human dental pulp-derived stem cells and their tissues of origin for banking and clinical use", Cryobiol., 59(2), 150-157. https://doi.org/10.1016/j.cryobiol.2009.06.005