DOI QR코드

DOI QR Code

Polymorphism Assessment of Six Lentil (Lens culinaris Medik.) Genotypes Using Isozyme

  • Madina, M. Hur (Department of Life Science, Chosun University) ;
  • Rahman, M. Saifur (Department of Pharmacy, Chosun University) ;
  • Deb, A. Chandra (Department of Genetic Engineering and Biotechnology, University of Rajshahi) ;
  • Choi, Yun Hee (Department of Pharmacy, Chosun University) ;
  • Kim, Mi Ri (Department of Pharmacy, Chosun University) ;
  • Shin, Jihoon (Department of Alternative Medicine, Gwangju University) ;
  • Yoo, Jin Cheol (Department of Pharmacy, Chosun University)
  • Received : 2015.05.28
  • Accepted : 2015.06.25
  • Published : 2015.06.30

Abstract

Lentil (Lens culinaris Medik.) is one of the important legumes and cheaper source of protein in Bangladesh that displays great biological diversity. Isozyme, one of the most important protein markers to detect genetic polymorphism in lentil, whereas we considered thirteen-isozyme in six varieties viz., BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. The highest polymorphism was found in tyrosinase isozyme system. UPGMA analysis revealed that the highest similarity between BARI masur-5 and BARI masur-6 whereas, the highest genetic distance between BARI masur-1 and BARI masur-5 reflecting higher intervarietal variation. Principal component analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 81.58%, 11.19% and 4.94% variation respectively, with cumulative variation of the first three PCs was 75.45%. Consequently, Isozyme could clearly assed the genetic diversity at intervarietal levels and these two varieties can be considered as valuable gene resources for future breeding and conservation programs.

Keywords

References

  1. N. Ahmed Kabashi, M. Alam, M. E. S. Mirghani, and A. M. A. Al-Fusaiel, "'Biodiesel production from crude palm oil by transesterification process'", Journal of Applied Sciences, Vol. 9, pp. 3166-3170, 2009. https://doi.org/10.3923/jas.2009.3166.3170
  2. G. C. Hawtin, K. B. Singh, and M. C. Saxena, "'Some recent developments in the understanding and improvement of Cicer and Lens'", Advances in legume science, R. J. Summerfield and A. H. Bunting, Eds, pp. 613-623, 1980.
  3. S. Nazir, E. L. Bashir, and R. Bantel, Eds, "Crop production", National Book Foundation'', 1994.
  4. M. S. Anjam, A. Ali, S. M. Iqbal, and A. M. Haqqani, "'Evaluation and correlation of economically important traits in exotic germplasm of lentil'", Int. J. Agric. Biol., Vol. 7, pp. 959-961, 2005.
  5. S. K. Sharma, I. K. Dawson, and R. Waugh, "'Relationships among cultivated and wild lentils revealed by RAPD analysis'", Theor. Appl. Genet., Vol. 91, pp. 647-654, 1995.
  6. R. N. Adsule, S. S. Kadam, and H. K. Leung, "Lentil, CRC Handbook of World Food Legumes: Nutritional Chemistry, Processing Technology, and Utilization", Vol. 2, pp. 133-152, 1989.
  7. R. J. Summerfield, E. H. Roberts, W. Erskine, and R. H. Ellis, "Effects of temperature and photoperiod on flowering in lentils (Lens culinaris Medic.)", Ann. Bot-rondon, Vol. 56, pp. 659-671, 1985. https://doi.org/10.1093/oxfordjournals.aob.a087055
  8. W. Erskine, Y. Adham, and L. Holly, "Geographic distribution of variation in quantitative traits in a world lentil collection", Euphytica, Vol. 43, pp. 97-103, 1989. https://doi.org/10.1007/BF00037901
  9. W. Erskine and M. A. Choudhary, "Variation between and within lentil landraces from Yemen Arab Republic", Euphytica, Vol. 35, pp. 695-700, 1986. https://doi.org/10.1007/BF00028577
  10. W. Erskine and F. J. Muehlbauer, "Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm", Theor. Appl. Genet., Vol. 83, pp.119-125, 1991.
  11. T. Sultana, A. Ghafoor, and M. Ashraf, "Geographic patterns of diversity of cultivated lentil germplasm collected from Pakistan, as assessed by seed protein assays", Acta Biol. Cracov. Bot., Vol. 48, pp. 77-84, 2006.
  12. F. M. Abdel-Tawab, H. Y. Olama, M. A. Rashed, S. H. Abdel-Aziz, S. A. N. Afiah, and S. H. Nesreen, "Marker assisted selection for salt tolerance in bread wheat (Triticum aestivum L.)", Al-Azhar Bull. Sci., Vol. 14, pp. 75-93, 2003.
  13. N. F. Weeden and J. F. Wendel, "Wendel, Genetics of plant isozymes". D.E. Soltis and P.S. Soltis, Eds, Isozymes in plant biology, Dioscorides Press, Portland, Oregon, USA, pp. 46-72, 1989.
  14. P. Jaccard, "Nouvelles recherches sur la distribution florale", 1908.
  15. A. Maquet, I. Z. Bi, M. Delvaux, B. Wathelet, and J.-P. Baudoin, "Genetic structure of a Lima bean base collection using allozyme markers", Theor. Appl. Genet., Vol. 95, pp. 980-991, 1997. https://doi.org/10.1007/s001220050651
  16. A. Sebastian, V. S. Sujatha, E. V. Nybe, G. S. Nair, and A. Augustine, "Isoenzyme variation and species relationship in the genus Piper", Journal of Tropical Agriculture, Vol. 34, pp. 136-137, 1996.
  17. M. D. Hayward and E. L. Breese, "Population structure and variability", Plant Breeding, Springer, Netherlands, pp. 16-29, 1993.
  18. M. Ehtisham-Ul-Haq, M. Munir, B. A. Malik, and N. M. Minhas, "Studies on Inheritance of some Isozymes in Lentil (Lens culinaris)", Pak. J. Bot., Vol. 33, pp. 181-186, 2001.
  19. M. K. Huh and H. W. Huh, "Genetic diversity and population structure of wild lentil tare", Crop Sci., Vol. 41, pp. 1940-1946, 2001. https://doi.org/10.2135/cropsci2001.1940
  20. M. Ipek and P. Simon, "Evaluation of genetic diversity among garlic clones using molecular markers: comparison of AFLPs, RAPDs and isozymes", Plant and animal genome X meeting, Jan, pp. 12-16, 2002.
  21. B. D. Prasad, G. Thapa, S. Baishya, and S. Sahni, "Biochemical and molecular characterization of submergence tolerance in rice for crop improvement", Journal of Plant Breeding and Crop Science, Vol. 3, pp. 240-250, 2011.
  22. S. Tuwafe, A. L. Kahier, A. Boe, and M. Ferguson, "Inheritance and geographical distribution of allozyme polymorphisms in chickpea (Cicer arietinum L.)", J. Hered., Vol. 79, pp. 170-174, 1988. https://doi.org/10.1093/oxfordjournals.jhered.a110487
  23. M. E. Haque, M. A. Islam, and B. Sikdar, "Molecular divergence of locally grown pumpkin (Cucurbita maxima) cultivars through some isozyme tests", Journal of Bio-Science, Vol. 19, pp. 89-93, 2011.
  24. M. A. Chowdhury and A. E. Slinkard, "Genetic diversity in grasspea (Lathyrus sativus L.)", Genet. Resour. Crop Ev., Vol. 47, pp.163-169, 2000. https://doi.org/10.1023/A:1008760604990
  25. M. Mohammadi, M. Aminipour, and Z. Banihashemi, "Isozyme analysis and soluble mycelial protein pattern in Iranian isolates of several formae speciales of Fusarium oxysporum", J. phytopathol., Vol. 152, pp. 267-276, 2004. https://doi.org/10.1111/j.1439-0434.2004.00839.x
  26. A. A. Jaradat and M. Shahid, "Population and multilocus isozyme structures in a barley landrace", Plant Genet. Resour-C, Vol. 4, pp. 108-116, 2006. https://doi.org/10.1079/PGR2005105
  27. S. Cheng, "Isozyme variation and inheritance in hazelnut", 1992.
  28. D. J. Coates and V. L. Hamley, "Genetic divergence and the mating system in the endangered and geographically restricted species, Lambertia orbifolia Gardner (Proteaceae)", Heredity, Vol. 83, pp. 418-427, 1999. https://doi.org/10.1038/sj.hdy.6885760
  29. R.-C. Yang, F. C. Yeh, and A. D. Yanchuk, "A comparison of isozyme and quantitative genetic variation in Pinus contorta ssp. latifolia by FST", Genetics, Vol. 142, pp. 1045-1052, 1996.
  30. M. Tahir, F. J. Muehlbauer, and S. C. Spaeth, "Association of isozyme markers with quantitative trait loci in random single seed descent derived lines of lentil (Lens culinaris Medik.)", Euphytica, Vol. 75, pp. 111-119, 1994. https://doi.org/10.1007/BF00024538
  31. A. Shamina, J. T. Zachariah, B. Sasikumar, and G. K. Johnson, '"Biochemical variation in turmeric (Curcuma longa Linn.) accessions based on isozyme polymorphism", J. Hortic. Sci. Biotech., Vol. 73, pp. 479-483, 1998. https://doi.org/10.1080/14620316.1998.11511002
  32. M. H. Madina, M. E. Haque, A. K. Dutta, M. A. Islam, A. C. Deb, and B. Sikdar, "Estimation of Genetic Diversity in Six Lentil (Lens culinaris Medik.) Varieties using Morphological and Biochemical markers", International Journal of Scientific & Engineering Research, Vol. 4, pp. 819-825, 2013.

Cited by

  1. Assessment of genetic diversity and phenetic relationships among some Bangladeshi cultivars of cucumber (Cucumis sativus L.) using isozyme and protein profiling vol.32, pp.4, 2019, https://doi.org/10.1007/s42535-019-00065-w