동아시아식생활학회지 (Journal of the East Asian Society of Dietary Life)

  • 제25권3호
  • /
  • Pages.451-459
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  • 2015
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  • 1225-6781(pISSN)
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  • 2288-8802(eISSN)
동아시아식생활학회 (The East Asian Society of Dietary Life)
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생팥 및 삶은 팥의 열수 추출물의 유용 생리활성 평가

Evaluation of Useful Biological Activities of Hot-Water Extracts of Raw-Red Bean and Boiled-Red Bean (Phaseolus radiatus L.)

  • Jung, In-Chang (Dept. of Food and Nutrition, Andong National University) ;
  • Lee, Ye-Seul (Dept. of Food and Nutrition, Andong National University) ;
  • Kang, Dong-Kyoon (GyeongSangBukDo Agricultural Research & Extension Services) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 투고 : 2015.03.12
  • 심사 : 2015.05.01
  • 발행 : 2015.06.30
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초록

식품으로 사용되는 팥은 생팥이 아닌 증자팥을 사용하며, 고온의 열수로 삶은 후 제조된다. 본 연구에서는 팥을 이용한 고부가가치 식품개발 연구의 일환으로, 생팥의 열수 추출물 및 팥을 삶은 후 증자액을 폐기하고 제조한 증자팥의 열수 추출물을 조제하고 이들의 유용성분 및 항산화, 항균, 항당뇨 및 항혈전 활성을 비교 평가하였다. 생팥 및 증자팥의 추출효율은 각각 16.2% 및 14.8%로 생팥이 1.1배 높았으며, 총 폴리페놀, 총 플라보노이드 및 환원당 함량은 생팥이 증자팥보다 각각 2.5배, 2.1배 및 1.5배 높았다. 생팥의 DPPH 음이온 및 ABTS 양이온 소거능 및 환원력 평가 결과, 증자팥보다 모두 높게 나타나, 팥의 항산화 활성이 고온의 열수 삶기 과정 중 소실됨을 알 수 있었다. 반면, nitrite 소거능은 생팥보다 삶은 증자팥에서 더욱 강하게 나타났다. 항균 활성 평가결과, 생팥의 열수 추출물에서만 그람양성 세균에 대한 활성이 나타났으며, 증자팥의 경우 항균 활성은 $500{\mu}g/disc$ 농도까지 나타나지 않았다. 또한 in-vitro $\alpha$-amylase 및 $\alpha$-glucosidase에 대한 저해활성과 항혈전 활성에 관련된 thrombin, prothrombin, coagulation factors에 대한 저해활성 평가 결과, 생팥의 비가열 추출물과는 달리, 생팥 및 증자팥의 열수 추출물은 전분분해 저해 및 항혈전 활성이 인정되지 않았다. 본 연구결과는 생팥 및 증자팥의 열수 추출물의 항산화, 항당뇨 및 항혈전 활성이 비가열 에탄올 추출물보다 미약하며, 생팥의 유용활성이 증자팥에서는 거의 남아 있지 않음을 제시하고 있으며, 팥을 이용한 고부가가치 식품 제조 시 적합한 삶기 공정 및 팥 증자액의 효율적인 이용에 대한 연구가 필요함을 제시하고 있다.

Raw-red bean (RR) should be boiled in hot water, and only boiled-red bean (BR) has been used in the food industry. In the course of development of functional food using red- bean (Phaseolus radiatus L), hot- water extracts (HWEs) of RR and BR were prepared, respectively and their components and various biological activities were compared. The extraction yield at $100^{\circ}C$ of RR (16.2%) was higher than that of BR (14.8%), and contents of total polyphenols, total flavonoids and reducing sugars of HWE of RR were 2.5-fold, 2.1-fold and 1.5-fold higher than those of HWE of BR. In anti-oxidation activity assay, scavenging activities against DPPH anion and ABTS cation as well as reducing power of RR was higher than those of BR. The results suggest that the anti-oxidant compounds in red bean might be heat-liable or discarded during boiling in hot-water as a cooking drip. Unexpectedly, nitrite scavenging activity was stronger in HWE of BR than RR. In anti-microbial activity assay, HWE of RR ($500{\mu}g/disc$) showed growth inhibition activity against gram-positive bacteria, whereas HWE of BR did not show any activity against any tested bacteria and fungi. Assay of in-vitro anti-diabetes and anti-thrombosis activities, which were previously reported in ethanol extract of red-bean, revealed that HWEs of RR and BR did not show significant activities against ${\alpha}$-amylase, ${\alpha}$-glucosidase, thrombin, prothrombin, or blood coagulation factors. Our results suggest that the anti-oxidation, anti-diabetes and anti-thrombosis activities of HWEs of RR and BR were lower than those of ethanol extracts of red bean, and bioactive substances in RR were destroyed during boiling or discarded after boiling. Further research on suitable boiling and re-use of cooking drip of red bean is necessary.

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참고문헌

  1. Ahn SM, Hong YK, Kwon GS, Sohn HY (2011) Evaluation of antioxidant and nitrite scavenging activity of seaweed extracts. J Life Sci 21: 576-583. https://doi.org/10.5352/JLS.2011.21.4.576
  2. Bae DG, Jung YS (2010) Colorant extracting and its storage stability from red bean and black bean seed coat. Agric Rex Bull Kyungpook Natl Univ 28: 31-38.
  3. Choi YH, Kang MY, Nam SH (1998) Inhibitory effect of various cereal and bean extracts on carcinogenicity in vitro. Korean J Food Sci Technol 30: 964-969.
  4. Choi SY, Jeong YJ, Lee SJ, Chi OH, Chegal SA (2002) Food and Health for Modern People. Dongmyungsa, Seoul, Korea. pp. 244-246.
  5. Grant G, More LJ, McKenzie NH, Stewart JC, Pusztai A (1983) A survey of the nutritional and haemagglutination properties of legume seeds generally available in the UK. Br J Nutr 50: 207-214. https://doi.org/10.1079/BJN19830090
  6. Hori Y, Sato S, Hatai A (2006) Antibacterial activity of plant extracts from azuki beans (Vigna angularis) in vitro. Phytother Res 20: 162-164. https://doi.org/10.1002/ptr.1826
  7. Hwang E, Park SY, Lee HJ, Sun ZW, Lee TY, Song HG, Shin HS, Yi TH (2014) Vigna angularis water extracts protect against ultraviolet B-exposed skin aging in vitro and in vivo. J Med Food 17:1339-1349. https://doi.org/10.1089/jmf.2013.3017
  8. Hwang JT, Oh HM, Kim MH, Jeong HJ, Lee C, Jang HJ, Lee SW, Park CS, Rho MC (2014) Simultaneous quantitation and validation of triterpenoids and phytosteroids in Phaseolus angularis seeds. Molecules 19: 10309-10319. https://doi.org/10.3390/molecules190710309
  9. Itoh T, Kobayashi M, Horio F, Furuichi Y (2009) Hypoglycemic effect of hot-water extract of adzuki (Vigna angularis) in spontaneously diabetic KK-A(y) mice. Nutrition 25: 134-141. https://doi.org/10.1016/j.nut.2008.08.001
  10. Itoh T, Nakamura M, Nakamichi H, Ando M, Tsukamasa Y, Furuichi Y(2014) Regulation of the differentiation of osteoblasts and osteoclasts by a hot-water extract of adzuki beans (Vigna angularis). Biosci Biotechnol Biochem 78: 92-99. https://doi.org/10.1080/09168451.2014.877182
  11. Jeong CH, Choi SG, Heo HJ (2008) Analysis of nutritional components and evaluation of functional activities of Sasa borealis leaf tea. Korean J Food Sci Technol 40: 586-592.
  12. Jeong SH, Kim SH, Kim HK, Yun BR, Lee HW, Lim JH, Rho MC, Kim DH (2012) Effect of Vigna anugularis ethanol extract on papain-induced arthritis in mice. Korean J Oriental Physiol Pathol 26: 665-671.
  13. Jung IC, Sohn HY (2014) Antioxidation, antimicrobial and antithrombosis activities of aged black garlic (Allium sativum L.). Korean J Microbiol Biotechnol 42: 285-292. https://doi.org/10.4014/kjmb.1407.07002
  14. Kang SJ, Han YS (2012) Studies on the anti oralmicrobial activity and selected functional component of small red bean extract. Korean J Food Cookery Sci 28: 41-49. https://doi.org/10.9724/kfcs.2012.28.1.041
  15. Kim CK, Oh BH, Na JM, Sin DH (2003) Comparison of physicochemical properties of Korean and Chinese red bean starches. Korean J Food Sci Technol 35: 551-555.
  16. Kim M, Park JE, Song SB, Cha YS (2015) Effects of black adzuki bean (Vigna angularis) extract on proliferation and differentiation of 3T3-L1 preadipocytes into mature adipocytes. Nutrients 7: 2772-2792.
  17. Koh KJ, Shin DB, Lee YC (1997) Physicochemical properties of aqueous extracts in small red bean, mung bean and black soybean. Korean J Food Sci Technol 29: 854-859.
  18. Lee HK, Hwang IG, Kim HY, Woo KS, Lee SH, Woo SH, Lee JS, Jeong HS (2010) Physicochemical characteristics and antioxidant activities of cereals and legumes in Korea. J Korean Soc Food Sci Nutr 39: 1399-1404. https://doi.org/10.3746/jkfn.2010.39.9.1399
  19. Lee RK, Kim MS, Lee YS, Lee MH, Lee JH, Sohn HY (2014) A comparison of the components and biological activities in raw and boiled red beans (Phaseolus radiates L.). Korean J Microbiol Biotechnol 42: 162-169. https://doi.org/10.4014/kjmb.1402.02006
  20. Mukai Y, Sato S (2009) Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveilin-1 expression in rats with hypertension. Nutr Metab Cardiovasc Dis 19: 491-497. https://doi.org/10.1016/j.numecd.2008.09.007
  21. Mukai Y, Sato S (2011) Polyphenol-containing azuki bean (Vigna angularis) seed coat attenuates vascular oxidative stress and inflammation in spontaneously hypertensive rats. J Nutr Biochem 22: 16-21. https://doi.org/10.1016/j.jnutbio.2009.11.004
  22. Oh HS, Kim JH, Lee MH (2003) Isoflavone contents, antioxidative and fibrinolytic activities of red bean and mung bean. Korean J Soc Food Cookery Sci 19: 263-270.
  23. Park YM, Jeong JB, Seo JH, Lim JH, Jeong HJ, Seo EW (2011) Inhibitory effect of red bean (Phaseolus angularis) hot water extracts on oxidative DNA and cell damage. Korean J Plant Res 24: 130-138. https://doi.org/10.7732/kjpr.2011.24.2.130
  24. Song SB, Ko JY, Kim JI, Lee JS, Jung TW, Kim KY, Kwak DY, Oh IS, Woo KS (2013) Changes in physicochemical characteristics and antioxidant activity of Adzuki bean and Adzuki bean tea depending on the variety and roasting time. Korean J Food Sci Technol 45: 317-324. https://doi.org/10.9721/KJFST.2013.45.3.317
  25. Song SB, Seo HI, Ko JY, Lee JS, Kang JR, Oh BG, Seo MC. Yoon YN, Kwak DY, Nam MH, Woo KS (2011) Quality characteristics of Adzuki bean sediment according to variety. J Korean Soc Food Sci Nutr 40: 1121-1127. https://doi.org/10.3746/jkfn.2011.40.8.1121
  26. Tanaka Y, Tsunogae Y, Hayakawa Y, Tanaka I, Yamane T, Ashida T, Ishikawa C, Watanabe K, Nakamura S, Takahashi K (1983) Preliminary crystallographic study of Bowman-Birk protease inhibitor (adzuki bean) and its complex with trypsin. J Biochem 94: 611-613. https://doi.org/10.1093/oxfordjournals.jbchem.a134394
  27. Valentina U, Facic J, Stampar F (2007) Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem 107: 185-192.
  28. Woo KS, Song SB, Ko JY, Seo MC, Lee JS, Kang JR, Oh BG, Nam MH, Jeong HS, Lee J (2010) Antioxidant components and antioxidant activities in methanolic extract from adzuki beans (Vigna angularis var. nipponensis). Korean J Food Sci Technol 42: 693-698.
  29. Yoshida C, Yoshikawa M (1975) Purification and characterization of proteinase inhibitors from adzuki beans (Phaseolus angularis). J Biochem 78: 935-945. https://doi.org/10.1093/oxfordjournals.jbchem.a131000
  30. Yu T, Ahn HM, Shen T, Yoon K, Jang HJ, Lee YJ, Yang HM, Kim JH, Kim C, Han MH, Cha SH, Kim TW, Kim SY, Lee J, Cho JY (2011) Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans. J Ethnopharmacol 137:1197-1206. https://doi.org/10.1016/j.jep.2011.07.048

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