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상백피(Morus alba root barks)로부터 페놀화합물의 분리 및 동정

Isolation and Identification of Phenolic Compounds from the Root Bark of Morus alba L.

  • Jung, Jae-Woo (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Park, Ji-Hae (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Baek, Yoon-Su (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Oh, Eun-Ji (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lim, Dong-Wook (Division of Matabolism and Functionality Research, Korea Food Research Institute) ;
  • Han, Daeseok (Division of Matabolism and Functionality Research, Korea Food Research Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
  • 투고 : 2015.02.04
  • 심사 : 2015.03.17
  • 발행 : 2015.06.30

초록

뽕나무(Morus alba L.) 뿌리껍질을 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAC, n-BuOH, 그리고 $H_2O$ 분획으로 나누었다. EtOAc 및 n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복 실시하여 4종의 phenolic 화합물을 분리, 정제하였다. NMR, IR, 및 EI/MS 등을 해석하여, norartocarpanone (1), 2',4',7-trihydroxy-(2S)-flavanone (2), methyl-${\beta}$-resorcylate (3), 그리고 (Z)-oxyresveratrol-4-O-${\beta}$-$\small{D}$-glucopyranoside (4)로 각각 구조동정하였다. 화합물 (Z)-oxyresveratrol-4-O-${\beta}$-$\small{D}$-glucopyranoside (4)는 상백피로부터는 이번 실험에서 처음으로 분리되었다.

The root barks of Morus alba L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$ fractions. The repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies of the EtOAc and n-BuOH fractions led to isolation of four phenolic compounds. The chemical structures of the compounds were determined as norartocarpanone (1), 2',4',7-trihydroxy-(2S)-flavanone (2), methyl ${\beta}$-resorcylate (3), and (Z)-oxyresveratrol-4-O-${\beta}$-$\small{D}$-glucopyranoside (4). Compound 4 was isolated for the first time from the root barks of M. alba L.

키워드

참고문헌

  1. Hakim EH, Achmad SA, Aimi N, Indrayanto G, Kitajima M, and Makmur L (2004) Regioselective glucosylation of oxyresveratrol by cell suspension cultures of Solanum mammosum. J Chem Res 2004, 706-7. https://doi.org/10.3184/0308234043431825
  2. Jung JW, Park JH, Jung YJ, Lee CH, Han D, and Baek NI (2014) Isolation and identification of triterpenoids from the Mulberry (Morus alba) root bark. J Appl Biol Chem 57, 295-9. https://doi.org/10.3839/jabc.2014.046
  3. Kang KB, Kim SD, Kim TB, Jeong EJ, Kim YC, Sung JH et al. (2011) Tyrosinase inhibitory constituents of Morus bombycis cortex. Natl Prod Res 17, 198-201.
  4. Zheng ZP, Chem S, Wang S, Wang XC, Cheng KW, Wu JJ et al. (2009) Chemical components and tyrosinase inhibitors from the twigs of Artocarpus heterophyllus. J Agric Food Chem 57, 6649-55. https://doi.org/10.1021/jf9014685

피인용 문헌

  1. One New Veratramine-Type Alkaloid From Veratrum maackii var. japonicum and Antioxidative Activities of Isolated Compounds vol.15, pp.7, 2020, https://doi.org/10.1177/1934578x20939408
  2. Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems vol.26, pp.14, 2015, https://doi.org/10.3390/molecules26144212