Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Low Density Lipoprotein-oxidation Inhibitory Phytochemicals from the Fruits of Rhus parviflora

  • Shrestha, Sabina (Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Park, Ji-Hae (Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Cho, Jin-Gyeong (Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Dae-Young (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kang, Ji-Hyun (National Research Laboratory of Lipid Metabolism & Atherosclerosis, KRIBB) ;
  • Li, Hua (National Research Laboratory of Lipid Metabolism & Atherosclerosis, KRIBB) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, KRIBB) ;
  • Kim Cho, Somi (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Lee, Dong-Sun (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Baek, Nam-In (Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University)
  • Received : 2015.01.03
  • Accepted : 2015.01.23
  • Published : 2015.06.30
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Abstract

Fruits of Rhus parviflora were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. Purification of EtOAc fraction led to isolation of fifteen polyphenols of which structures were identified by spectroscopic methods including 2D-NMR. Most compounds apart from compound 10 inhibited low density lipoproteinoxidation within $IC_{50}$ value of $10{\mu}M$. Among compounds, taxifolin (2), quercetin 3-O-${\alpha}$-L-rhamnopyranoside (13), agathisflavone (5) sulfuretin (4), and aureusidin (3) showed $IC_{50}$ values 0.9, 0.8, 5.8, 2.9, and $2.4{\mu}M$ which were of highly significant in comparison positive control butylated hydroxytoluene with $IC_{50}$ value of $2.1{\mu}M$. The results indicate fruits of R. parviflora as a source of antihypercholesterolemic compounds.

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References

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