Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Chitin from Cuttlebone Activates Inflammatory Cells to Enhance the Cell Migration

  • Lim, Sung Cil (College of Pharmacy, The Catholic University of Korea) ;
  • Lee, Ki-Man (Institute of Chronic Diseases and College of Pharmacy, Sahmyook University) ;
  • Kang, Tae Jin (Institute of Chronic Diseases and College of Pharmacy, Sahmyook University)
  • Received : 2015.05.26
  • Accepted : 2015.06.08
  • Published : 2015.07.01
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Abstract

Our previous report showed that the extract from cuttlebone (CB) had wound healing effect in burned lesion of rat and the extract was identified as chitin by HPLS analysis. We herein investigated the morphology in CB extract using scanning electron microscope (SEM). Chitin was used as a control. There is no difference in morphology between CB extract and chitin. We also assessed the role of CB extract on the production of inflammatory mediators using murine macrophages and the migration of inflammatory cells. The extract induced the production of nitric oxide (NO) in macrophages. While the extract of CB itself stimulated macrophages to increase the expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6, CB extract suppressed the production of those cytokines by LPS. CB extract also induced the production of mouse IL-8 which is related to the cell migration, and treatment with CB enhanced fibroblast migration and invasion. Therefore, our results suggest that CB activates inflammatory cells to enhance the cell migration.

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References

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