References
- Bae, S., Park, J., and Kim, J.S. (2014). Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30, 1473-1475. https://doi.org/10.1093/bioinformatics/btu048
- Bibikova, M., Beumer, K., Trautman, J.K., and Carroll, D. (2003). Enhancing gene targeting with designed zinc finger nucleases. Science 300, 764. https://doi.org/10.1126/science.1079512
- Bitinaite, J., Wah, D.A., Aggarwal, A.K., and Schildkraut, I. (1998).FokI dimerization is required for DNA cleavage. Proc. Natl. Acad. Sci. USA 95, 10570-10575. https://doi.org/10.1073/pnas.95.18.10570
- Boch, J., Scholze, H., Schornack, S., Landgraf, A., Hahn, S., Kay, S., Lahaye, T., Nickstadt, A., and Bonas, U. (2009). Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326, 1509-1512. https://doi.org/10.1126/science.1178811
- Brunet, E., Simsek, D., Tomishima, M., DeKelver, R., Choi, V.M., Gregory, P., Urnov, F., Weinstock, D.M., and Jasin, M. (2009). Chromosomal translocations induced at specified loci in human stem cells. Proc. Natl. Acad. Sci. USA 106, 10620-10625. https://doi.org/10.1073/pnas.0902076106
- Cho, S.W., Kim, S., Kim, J.M., and Kim, J.S. (2013a). Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat. Biotechnol. 31, 230-232. https://doi.org/10.1038/nbt.2507
- Cho, S.W., Lee, J., Carroll, D., Kim, J.S., and Lee, J. (2013b). Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics 195, 1177-1180. https://doi.org/10.1534/genetics.113.155853
- Cho, S.W., Kim, S., Kim, Y., Kweon, J., Kim, H.S., Bae, S., and Kim, J.S. (2014). Analysis of off-target effects of CRISPR/Casderived RNA-guided endonucleases and nickases. Genome Res. 24, 132-141. https://doi.org/10.1101/gr.162339.113
- Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823. https://doi.org/10.1126/science.1231143
- Cradick, T.J., Fine, E.J., Antico, C.J., and Bao, G. (2013) CRISPR/as9 systems targeting beta-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res. 41, 9584-9592. https://doi.org/10.1093/nar/gkt714
- Crosetto, N., Mitra, A., Silva, M.J., Bienko, M., Dojer, N., Wang, Q., Karaca, E., Chiarle, R., Skrzypczak, M., Ginalski, K., et al. (2013). Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing. Nat. Methods 10, 361-365. https://doi.org/10.1038/nmeth.2408
- Frock, R.L., Hu, J., Meyers, R.M., Ho, Y.J., Kii, E., and Alt, F.W. (2015). Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nat. Biotechnol. 33, 179-186. https://doi.org/10.1038/nbt.3101
- Fu, Y., Foden, J.A., Khayter, C., Maeder, M.L., Reyon, D., Joung, J.K., and Sander, J.D. (2013). High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826. https://doi.org/10.1038/nbt.2623
- Fu, Y., Sander, J.D., Reyon, D., Cascio, V.M., and Joung, J.K. (2014). Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat. Biotechnol. 32, 279-284. https://doi.org/10.1038/nbt.2808
- Gabriel, R., Lombardo, A., Arens, A., Miller, J.C., Genovese, P., Kaeppel, C., Nowrouzi, A., Bartholomae, C.C., Wang, J., Friedman, G., et al. (2011). An unbiased genome-wide analysis of zincfinger nuclease specificity. Nat. Biotechnol. 29, 816-823. https://doi.org/10.1038/nbt.1948
- Hendel, A., Kildebeck, E.J., Fine, E.J., Clark, J.T., Punjya, N., Sebastiano, V., Bao, G., and Porteus, M.H. (2014). Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep. 7, 293-305. https://doi.org/10.1016/j.celrep.2014.02.040
- Hsu, P.D., Scott, D.A., Weinstein, J.A., Ran, F.A., Konermann, S., Agarwala, V., Li, Y., Fine, E.J., Wu, X., Shalem, O., et al. (2013). DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832. https://doi.org/10.1038/nbt.2647
- Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., and Charpentier, E. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821. https://doi.org/10.1126/science.1225829
- Jinek, M., East, A., Cheng, A., Lin, S., Ma, E., and Doudna, J. (2013). RNA-programmed genome editing in human cells. Elife 2, e00471.
- Kim, H., and Kim, J.S. (2014). A guide to genome engineering with programmable nucleases. Nat. Rev. Genet. 15, 321-334. https://doi.org/10.1038/nrg3686
- Kim, Y.G., Cha, J., and Chandrasegaran, S. (1996). Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Proc. Natl. Acad. Sci. USA 93, 1156-1160. https://doi.org/10.1073/pnas.93.3.1156
- Kim, H.J., Lee, H.J., Kim, H., Cho, S.W., and Kim, J.S. (2009). Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Genome Res. 19, 1279-1288. https://doi.org/10.1101/gr.089417.108
- Kim, J.S., Lee, H.J., and Carroll, D. (2010). Genome editing with modularly assembled zinc-finger nucleases. Nat. Methods 7, 91; author reply 91-92.
- Kim, H., Um, E., Cho, S.R., Jung, C., and Kim, J.S. (2011). Surrogate reporters for enrichment of cells with nucleaseinduced mutations. Nat. Methods 8, 941-943. https://doi.org/10.1038/nmeth.1733
- Kim, E., Kim, S., Kim, D.H., Choi, B.S., Choi, I.Y., and Kim, J.S. (2012). Precision genome engineering with programmable DNA-nicking enzymes. Genome Res. 22, 1327-1333. https://doi.org/10.1101/gr.138792.112
- Kim, Y., Kweon, J., Kim, A., Chon, J.K., Yoo, J.Y., Kim, H.J., Kim, S., Lee, C., Jeong, E., Chung, E., et al. (2013a). A library of TAL effector nucleases spanning the human genome. Nat. Biotechnol. 31, 251-258. https://doi.org/10.1038/nbt.2517
- Kim, Y., Kweon, J., and Kim, J.S. (2013b). TALENs and ZFNs are associated with different mutation signatures. Nat. Methods 10, 185. https://doi.org/10.1038/nmeth.2364
- Kim, Y.K., Wee, G., Park, J., Kim, J., Baek, D., Kim, J.S., and Kim, V.N. (2013c). TALEN-based knockout library for human microRNAs. Nat. Struct. Mol. Biol. 20, 1458-1464. https://doi.org/10.1038/nsmb.2701
- Kim, J.M., Kim, D., Kim, S., and Kim, J.S. (2014a). Genotyping with CRISPR-Cas-derived RNA-guided endonucleases. Nat. Commun. 5, 3157.
- Kim, S., Kim, D., Cho, S.W., Kim, J., and Kim, J.S. (2014b). Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24, 1012- 1019. https://doi.org/10.1101/gr.171322.113
- Kim, D., Bae, S., Park, J., Kim, E., Kim, S., Yu, H.R., Hwang, J., Kim, J.I. and Kim, J.S. (2015). Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237-243. https://doi.org/10.1038/nmeth.3284
- Kuscu, C., Arslan, S., Singh, R., Thorpe, J., and Adli, M. (2014). Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat. Biotechnol. 32, 677-683. https://doi.org/10.1038/nbt.2916
- Lee, H.J., Kim, E., and Kim, J.S. (2010). Targeted chromosomal deletions in human cells using zinc finger nucleases. Genome Res. 20, 81-89. https://doi.org/10.1101/gr.099747.109
- Lee, H.J., Kweon, J., Kim, E., Kim, S. and Kim, J.S. (2012) .Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases. Genome Res. 22, 539-548. https://doi.org/10.1101/gr.129635.111
- Lin, Y., Cradick, T.J., Brown, M.T., Deshmukh, H., Ranjan, P., Sarode, N., Wile, B.M., Vertino, P.M., Stewart, F.J. and Bao, G. (2014). CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res. 42, 7473-7485. https://doi.org/10.1093/nar/gku402
- Mali, P., Aach, J., Stranges, P.B., Esvelt, K.M., Moosburner, M., Kosuri, S., Yang, L. and Church, G.M. (2013a). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat. Biotechnol. 31, 833-838. https://doi.org/10.1038/nbt.2675
- Mali, P., Yang, L., Esvelt, K.M., Aach, J., Guell, M., DiCarlo, J.E., Norville, J.E., and Church, G.M. (2013b). RNA-guided human genome engineering via Cas9. Science 339, 823-826. https://doi.org/10.1126/science.1232033
- Miller, J.C., Tan, S., Qiao, G., Barlow, K.A., Wang, J., Xia, D.F., Meng, X., Paschon, D.E., Leung, E., Hinkley, S.J., et al. (2011). A TALE nuclease architecture for efficient genome editing. Nat. Biotechnol. 29, 143-148. https://doi.org/10.1038/nbt.1755
- Moscou, M.J., and Bogdanove, A.J. (2009). A simple cipher governs DNA recognition by TAL effectors. Science 326, 1501. https://doi.org/10.1126/science.1178817
- Mussolino, C., Morbitzer, R., Lutge, F., Dannemann, N., Lahaye, T. and Cathomen, T. (2011). A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res. 39, 9283-9293. https://doi.org/10.1093/nar/gkr597
- Parant, J.M., George, S.A., Pryor, R., Wittwer, C.T., and Yost, H.J. (2009). A rapid and efficient method of genotyping zebrafish mutants. Dev. Dyn. 238, 3168-3174. https://doi.org/10.1002/dvdy.22143
- Park, C.Y., Kim, J., Kweon, J., Son, J.S., Lee, J.S., Yoo, J.E., Cho, S.R., Kim, J.H., Kim, J.S., and Kim, D.W. (2014). Targeted inversion and reversion of the blood coagulation factor 8 gene in human iPS cells using TALENs. Proc. Natl. Acad. Sci. USA 111, 9253-9258. https://doi.org/10.1073/pnas.1323941111
- Pattanayak, V., Ramirez, C.L., Joung, J.K., and Liu, D.R. (2011). Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat. Methods 8, 765-770. https://doi.org/10.1038/nmeth.1670
- Pattanayak, V., Lin, S., Guilinger, J.P., Ma, E., Doudna, J.A., and Liu, D.R. (2013). High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol. 31, 839-843. https://doi.org/10.1038/nbt.2673
- Ramakrishna, S., Kwaku Dad, A.B., Beloor, J., Gopalappa, R., Lee, S.K., and Kim, H. (2014). Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res. 24, 1020-1027. https://doi.org/10.1101/gr.171264.113
- Ran, F.A., Hsu, P.D., Lin, C.Y., Gootenberg, J.S., Konermann, S., Trevino, A.E., Scott, D.A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013). Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154, 1380-1389. https://doi.org/10.1016/j.cell.2013.08.021
- Ran, F.A., Cong, L., Yan, W.X., Scott, D.A., Gootenberg, J.S., Kriz, A.J., Zetsche, B., Shalem, O., Wu, X., Makarova, K.S., et al. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature 520, 186-191. https://doi.org/10.1038/nature14299
- Smith, C., Gore, A., Yan, W., Abalde-Atristain, L., Li, Z., He, C., Wang, Y., Brodsky, R.A., Zhang, K., Cheng, L., et al. (2014). Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Cell Stem Cell 15, 12-13. https://doi.org/10.1016/j.stem.2014.06.011
- Tebas, P., Stein, D., Tang, W.W., Frank, I., Wang, S.Q., Lee, G., Spratt, S.K., Surosky, R.T., Giedlin, M.A., Nichol, G., et al. (2014). Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl. J. Med. 370, 901-910. https://doi.org/10.1056/NEJMoa1300662
- Tsai, S.Q., Zheng, Z., Nguyen, N.T., Liebers, M., Topkar, V.V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A.J., Le, L.P., et al. (2015). GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat. Biotechnol. 33, 187-197. https://doi.org/10.1038/nbt.3117
- Urnov, F.D., Miller, J.C., Lee, Y.L., Beausejour, C.M., Rock, J.M., Augustus, S., Jamieson, A.C., Porteus, M.H., Gregory, P.D., and Holmes, M.C. (2005). Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 435, 646-651. https://doi.org/10.1038/nature03556
- Veres, A., Gosis, B.S., Ding, Q., Collins, R., Ragavendran, A., Brand, H., Erdin, S., Cowan, C.A., Talkowski, M.E., and Musunuru, K. (2014). Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing. Cell Stem Cell 15, 27-30. https://doi.org/10.1016/j.stem.2014.04.020
- Vouillot, L., Thelie, A., and Pollet, N. (2015). Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407-415. https://doi.org/10.1534/g3.114.015834
- Wu, X., Scott, D.A., Kriz, A.J., Chiu, A.C., Hsu, P.D., Dadon, D.B., Cheng, A.W., Trevino, A.E., Konermann, S., Chen, S., et al. (2014). Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat. Biotechnol. 32, 670-676. https://doi.org/10.1038/nbt.2889
- Yusa, K., Rashid, S.T., Strick-Marchand, H., Varela, I., Liu, P.Q., Paschon, D.E., Miranda, E., Ordonez, A., Hannan, N.R., Rouhani, F.J., et al. (2011). Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent stem cells. Nature 478, 391-394. https://doi.org/10.1038/nature10424
- Zhu, X., Xu, Y., Yu, S., Lu, L., Ding, M., Cheng, J., Song, G., Gao, X., Yao, L., Fan, D., et al. (2014). An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Sci. Rep. 4, 6420. https://doi.org/10.1038/srep06420
- Zuris, J.A., Thompson, D.B., Shu, Y., Guilinger, J.P., Bessen, J.L., Hu, J.H., Maeder, M.L., Joung, J.K., Chen, Z.Y. and Liu, D.R. (2015). Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat. Biotechnol. 33, 73-80. https://doi.org/10.1038/nbt.3081
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- Gene Silencing Strategies in Cancer Therapy: An Update for Drug Resistance vol.26, pp.34, 2015, https://doi.org/10.2174/0929867325666180403141554
- Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants vol.10, pp.None, 2015, https://doi.org/10.3389/fpls.2019.00550
- Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system vol.9, pp.16, 2015, https://doi.org/10.1039/c8ra10017a
- Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system vol.9, pp.16, 2015, https://doi.org/10.1039/c8ra10017a
- The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases vol.17, pp.None, 2015, https://doi.org/10.1016/j.csbj.2019.06.006
- Target-dependent nickase activities of the CRISPR-Cas nucleases Cpf1 and Cas9 vol.4, pp.5, 2015, https://doi.org/10.1038/s41564-019-0382-0
- Biallelic mutations in USP45, encoding a deubiquitinating enzyme, are associated with Leber congenital amaurosis vol.56, pp.5, 2015, https://doi.org/10.1136/jmedgenet-2018-105709
- Therapeutic application of the CRISPR system: current issues and new prospects vol.138, pp.6, 2019, https://doi.org/10.1007/s00439-019-02028-2
- Efficient Gene Editing at Major CFTR Mutation Loci vol.16, pp.None, 2015, https://doi.org/10.1016/j.omtn.2019.02.006
- Genome-Editing Technologies: Concept, Pros, and Cons of Various Genome-Editing Techniques and Bioethical Concerns for Clinical Application vol.16, pp.None, 2015, https://doi.org/10.1016/j.omtn.2019.02.027
- Delivery of CRISPR/Cas9 for therapeutic genome editing vol.21, pp.7, 2015, https://doi.org/10.1002/jgm.3107
- Recent advances in the CRISPR genome editing tool set vol.51, pp.11, 2015, https://doi.org/10.1038/s12276-019-0339-7
- Mendelian disorders of the epigenetic machinery: postnatal malleability and therapeutic prospects vol.28, pp.r2, 2015, https://doi.org/10.1093/hmg/ddz174
- Utilization of the CRISPR-Cas9 Gene Editing System to Dissect Neuroinflammatory and Neuropharmacological Mechanisms in Parkinson’s Disease vol.14, pp.4, 2019, https://doi.org/10.1007/s11481-019-09844-3
- High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases vol.10, pp.1, 2019, https://doi.org/10.1038/s41467-019-09987-0
- CRISPR/Cas9-Mediated TERT Disruption in Cancer Cells vol.21, pp.2, 2015, https://doi.org/10.3390/ijms21020653
- Genome-wide Cas9 binding specificity in Saccharomyces cerevisiae vol.8, pp.None, 2015, https://doi.org/10.7717/peerj.9442
- CRISPR-Cas System: An Approach With Potentials for COVID-19 Diagnosis and Therapeutics vol.10, pp.None, 2020, https://doi.org/10.3389/fcimb.2020.576875
- CRISPR/Cas9 for development of disease resistance in plants: recent progress, limitations and future prospects vol.19, pp.1, 2015, https://doi.org/10.1093/bfgp/elz041
- Genome editing: the dynamics of continuity, convergence, and change in the engineering of life vol.39, pp.2, 2020, https://doi.org/10.1080/14636778.2020.1730166
- Recent advances in genome editing of stem cells for drug discovery and therapeutic application vol.209, pp.None, 2015, https://doi.org/10.1016/j.pharmthera.2020.107501
- CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment vol.41, pp.5, 2020, https://doi.org/10.1038/s41401-019-0322-9
- Dissecting the Therapeutic Relevance of Gene Therapy in NeuroAIDS: An Evolving Epidemic vol.20, pp.3, 2015, https://doi.org/10.2174/1566523220666200615151805
- CRISPR/Cas9 gene drive technology to control transmission of vector‐borne parasitic infections vol.42, pp.9, 2015, https://doi.org/10.1111/pim.12762
- A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors vol.42, pp.9, 2015, https://doi.org/10.1177/1010428320962588
- CD70 Inversely Regulates Regulatory T Cells and Invariant NKT Cells and Modulates Type 1 Diabetes in NOD Mice vol.205, pp.7, 2020, https://doi.org/10.4049/jimmunol.2000148
- TALEN mediated gene editing in a mouse model of Fanconi anemia vol.10, pp.None, 2020, https://doi.org/10.1038/s41598-020-63971-z
- Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides vol.21, pp.1, 2015, https://doi.org/10.1186/s13059-020-01956-x
- Genomic Engineering in Human Hematopoietic Stem Cells: Hype or Hope? vol.2, pp.None, 2015, https://doi.org/10.3389/fgeed.2020.615619
- Identifying genome-wide off-target sites of CRISPR RNA-guided nucleases and deaminases with Digenome-seq vol.16, pp.2, 2021, https://doi.org/10.1038/s41596-020-00453-6
- Applications and Potential of Genome-Editing Systems in Rice Improvement: Current and Future Perspectives vol.11, pp.7, 2021, https://doi.org/10.3390/agronomy11071359
- Genetic manipulations of AMPA glutamate receptors in hippocampal synaptic plasticity vol.194, pp.None, 2021, https://doi.org/10.1016/j.neuropharm.2021.108630
- Advances in generating HLA-Universal platelets for transfusion medicine vol.14, pp.None, 2015, https://doi.org/10.1016/j.regen.2021.100053