미생물학회지 (Korean Journal of Microbiology)

  • 제51권1호
  • /
  • Pages.14-20
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  • 2015
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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동해안 자생식물로부터 분리된 내생균류의 식물생장촉진활성 및 동정

Plant growth-promoting activity and identification of endophytic fungi isolated from native plant in East coast

  • 유영현 (경북대학교 생명과학부) ;
  • 진용주 (국립농업과학원 농업미생물과) ;
  • 강상모 (경북대학교 응용생명과학부) ;
  • 오세종 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이명철 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김종국 (경북대학교 생명과학부)
  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Jin, Yong Ju (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Sang-Mo (School of Applied Biosciences, Kyungpook National University) ;
  • Oh, Sejong (National Agrobiodiversity Center, NAAS, RDA) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, NAAS, RDA) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 투고 : 2015.02.09
  • 심사 : 2015.03.05
  • 발행 : 2015.03.31
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초록

동해안에 자생하는 해안식물인 개질경이를 채집하였고, 뿌리로부터 형태적으로 다른 내생균류 20균주를 선발하였다. 개질경이 뿌리에 공생하는 내생균류의 ITS-rDNA 염기서열을 분석하였으며, 분리된 내생균류의 유연관계는 Bayesian 프로그램을 이용하여 계통분석을 하였다. 모든 내생균류의 농축배양여과액을 난장이벼에 처리하여 식물생장촉진활성을 스크리닝하였고, 분리된 균류 중에서 E/PC/10/1 균주가 생장촉진활성이 우수한 것으로 확인되었다. E/PC/10/1 균주의 배양여과액을 HPLC와 GC/MS-SIM을 이용하여 분석한 결과, 식물호르몬인 지베렐린 $GA_1$, $GA_3$, $GA_4$가 정량분석 되었다. 그리고 E/PC/10/1 균주의 명확한 동정을 위하여, beta-tubulin 유전자 염기서열을 이용한 분자적인 방법과 현미경을 이용한 형태적인 방법으로 동정하였다. 최종적으로 E/PC/10/1 균주는 GAs을 생산하는 새로운 P. spinulosum으로 동정되었다.

Coastal plant species, Plantago camtschatica Cham. native to the coastal region of the East Sea were sampled and then morphologically different 20 endophytic fungal strains were purely isolated. Phylogenetic analysis of isolates was done by the Bayesian program based on sequenced internal transcribed spacer (ITS-rDNA) region. Culture filtrates of each of 20 isolates were treated to Waito-c rice (WR) seedlings for verifying plant growth-promoting activity, respectively. As the results, E/PC/10/1 strain showed the highest plant growth-promoting activity among them. The culture filtrate of the strain E/PC/10/1 was revealed as containing gibberellins ($GA_1$, $GA_3$, $GA_4$) by using HPLC, and gas GC/MS with selected ion monitoring (SIM). Finally, this strain was identified as novel Penicillium spinulosum species that producing new GAs with microscopic observation and further molecular analysis with beta-tubulin gene sequence.

키워드

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