KSBB Journal

  • 제30권1호
  • /
  • Pages.44-51
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  • 2015
  • /
  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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유전자재조합 CHO 세포에서 Histone Deacetylase Inhibitor를 이용한Albumin-erythropoietin 생산성 증진

Enhanced Production of Albumin-erythropoietin by Histone Deacetylase Inhibitors in Recombinant CHO Cells

  • 김수진 (인하대학교 공과대학 생물공학과) ;
  • 서준석 (인하대학교 공과대학 생물공학과) ;
  • 최성훈 (인하대학교 공과대학 생물공학과) ;
  • 차현명 (인하대학교 공과대학 생물공학과) ;
  • 임진혁 (인하대학교 공과대학 생물공학과) ;
  • 신수아 (인하대학교 공과대학 생물공학과) ;
  • 신연경 (인하대학교 공과대학 생물공학과) ;
  • 김동일 (인하대학교 공과대학 생물공학과)
  • Kim, Su-Jin (Department of Biological Engineering, Inha University) ;
  • Seo, Joon-Serk (Department of Biological Engineering, Inha University) ;
  • Choi, Sung-Hun (Department of Biological Engineering, Inha University) ;
  • Cha, Hyun-Myoung (Department of Biological Engineering, Inha University) ;
  • Lim, Jin-Hyuk (Department of Biological Engineering, Inha University) ;
  • Shin, Soo-Ah (Department of Biological Engineering, Inha University) ;
  • Shin, Yeon-Kyeong (Department of Biological Engineering, Inha University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • 투고 : 2015.01.05
  • 심사 : 2015.02.25
  • 발행 : 2015.02.27
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초록

Chinese hamster ovary (CHO) cells are the most widely used mammalian host for the commercial production of recombinant proteins. However, they show relatively low yields of recombinant proteins in comparison with microbial cells. Various strategies have been tried to overcome this drawback. The acetyl moieties are attached to the N-terminus of histone by histone acetyltransferase (HAT) while histone deacetylase (HDAC) removes histone-bound acetyl groups. HDAC inhibitor (HDACi), such as sodium butyrate, sodium propionate and valproic acid, can enhance specific productivity of CHO cells. Human albumin-erythropoietin (Alb-EPO) is a novel 105 kDa protein comprising recombinant human EPO fused to human albumin. In this study, we examined the effects of HDACi on the production of Alb-EPO in CHO cells with various concentrations in the range of 0-1 mM. The results showed that sodium butyrate was found to be the best HDACi for enhancing productivity. It enhanced not only the production of Alb-EPO but also the apoptosis of recombinant CHO cells.

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참고문헌

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