Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Optimum Treatment Parameters for Photodynamic Antimicrobial Chemotherapy on Streptococcus mutans Biofilms

Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구

  • Choi, Seojung (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Microbiology and Immunology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 최서정 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 이주현 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학 및 면역학교실 및 구강과학연구소)
  • Received : 2014.10.13
  • Accepted : 2015.01.20
  • Published : 2015.05.31
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Abstract

The purpose of this study was to evaluate the effects of Photochemotherapy using a combination of erythrosine and standard halogen dental curing lights on the viability of Streptococcus mutans in the biofilm phase. To investigate the optimum treatment parameters, the researchers controlled the concentration of erythrosine, light irradiation time and the treatment time of erythrosine. The higher concentration of erythrosine (0, 10, 20, 40, 80 M) in the presence of light irradiation created greater effects in reducing the viability of S. mutans. The results showed a statistically significant difference among the antimicrobial effects in 20, 40, 80 M erythrosine. The higher irradiation time of light (0, 5, 15, 30, 60, 75s) in the presence of erythrosine showed greater effects in reducing the viability of S. mutans. There was statistically significant difference in 30, 60, 75 seconds. The higher treatment time of erythrosine (0, 1, 2.5, 5min) in the presence of erythrosine created greater effects on reduction of S. mutans viability. Statistically significant differences were found between 2.5 and 5 minutes of erythrosine treatment time. The results of this study showed that the photochemotherapy on S. mutans using erythrosine and the halogen dental curing lights conventionally used in dental clinics is effective in the condition of 20-40 M erythrosine concentration, irradiation time over 30 seconds, and erythrosine treatment time over 2.5 minutes.

할로겐 광중합기를 광원으로, 치면세균막 착색제인 erythrosine을 광감각제로 사용하여 S. mutans biofilm에 대한 광역동 치료를 시행할 때의 최적조건을 알아보기 위해서 erythrosine의 농도와 광조사 시간 및 광감각제의 접촉시간에 따른 광역동 치료의 효과를 비교하였다. erythrosine의 농도를 0, 10, 20, 40, $80{\mu}M$로 변화시킨 결과 농도가 증가할수록 항균효과가 증가하는 경향을 보였으며 $40{\mu}M$과 $80{\mu}M$의 두 군에서 통계적 유의성이 나타났다. 광 조사 시간을 0, 5, 15, 30, 60, 75초로 변화시킨 결과 조사 시간이 길어질수록 항균효과가 증가하는 경향을 보였으며 30초, 60초, 75초의 세 군에서 통계적 유의성이 나타났다. erythrosine 처리시간을 0분, 1분, 2분30초, 5분으로 변화시킨 결과 erythrosine과의 접촉시간이 늘어날수록 항균효과가 증가하는 경향을 보였으며 2분30초 군과 5분 군에서 통계적 유의성을 보였다. 이상의 결과로 광감각제로 사용한 erythrosine의 농도를 $20-40{\mu}M$, 광원으로 사용한 할로겐 광중합기의 광 조사시간을 30초 이상, 광감각제인 erythrosine을 2분30초 이상 처리하여 사용하는 것이 치아우식 예방을 위한 광역동 치료에 효과적임을 알 수 있다.

Keywords

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