조미김의 제조공정별 위해요소분석, 중요관리점 결정 및 한계기준 개발

Hazard Analysis, Determination of Critical Control Points, and Establishment of Critical Limits for Seasoned Laver

  • 투고 : 2014.08.19
  • 심사 : 2015.04.14
  • 발행 : 2015.04.30

초록

본 연구에서는 조미김 식품제조 가공 업소에서 제조한 조미김을 대상으로 주요 원료 및 제조공정별 생물학적 위해요소분석을 실시하여 생물학적 중요관리점(CCP)을 파악하고, 이를 관리하고 모니터링하기 위한 한계기준을 설정하여 HACCP Plan을 제시하고자 한다. 본 연구 결과, 일반세균수는 2차 가열공정 이전에서는 $10^3{\sim}10^4CFU/g$의 높은 수준으로 검출되었으나, 2차 가열 이후에서는 $1.0{\times}10CFU/g$ 미만으로 검출되었다. 조미김의 제조공정별 병원성미생물 분석결과, 모든 제조공정에서 검출되지 않았다. 이와 같은 결과로 조미김의 제조 유통시 CCP는 2차 가열 과정으로 결정되었다. 가열온도를 $170{\pm}5^{\circ}C$, $200{\pm}0.5^{\circ}C$, $230{\pm}5^{\circ}C$로 하고, 각각의 온도에서 가열시간을 $3.0{\pm}5$초, $4.0{\pm}0.5$초, $5.5{\pm}0.5$초로 실시하면서 일반세균과 병원성미생물을 시험한 결과, 2차 가열 전 병원성 미생물은 음성이었다. 일반세균은 $230{\pm}5^{\circ}C$일 때 현저히 감소되었고, 특히 가열시간을 $5.5{\pm}0.5$일 때 $1.0{\times}10CFU/g$ 이하로 나타났다. 미생물학적 위해요소를 HACCP 시스템에 적용하여 생산한 조미김의 경우 상품가치와 품질, 안전성을 확보하기에 가장 적절한 가열온도와 가열시간은 $230{\pm}5^{\circ}C$, 가열시간은 $5.5{\pm}0.5$초로 한계기준을 설정하였다. 본 연구를 통하여 제조공정별 위해요소분석을 활용하여 조미김의 중요관리점 및 한계기준을 설정하였고, 이를 통하여 HACCP 계획을 수립하고, 사후관리 등에 활용할 수 있다.

The purpose of this study was to establish the critical limit of CCP (Critical Control Point) of a HACCP (Hazard Analysis Critical Control Point) system used in the production of seasoned laver products. The hazard analysis examined microbial evaluations and developed a HACCP management plan for the heating process. The results were determined to be capable of reducing the biological element of CCP via the secondary roasting process. This study examined general bacteria and pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, and Bacillus cereus at temperatures ranging from $170^{\circ}C$ to $230^{\circ}C$ and for 3.0 to 5.5 seconds at a time. Before the secondary roasting process, pathogenic microorganisms were all negative, although the presence of general bacteria was still detected. General bacteria was reduced to $1.0{\times}10CFU/g$ after the temperature was set at $230^{\circ}C$ for a period of 5.5 seconds. In conclusion, it suggested that a HACCP plan was necessary for management standard and systematic approach in the establishment of critical limit, problem resolution, verification method, education, and records management through a secondary roasting process.

키워드

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