The Journal of Advanced Prosthodontics

  • 제7권2호
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  • Pages.166-171
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  • 2015
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  • 2005-7806(pISSN)
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  • 2005-7814(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
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Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coating on titanium

  • Ji, Min-Kyung (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Park, Sang-Won (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Lee, Kwangmin (Department of Materials Science and Engineering, Chonnam National University) ;
  • Kang, In-Chol (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Yun, Kwi-Dug (Department of Prosthodontics, School of Dentistry, Chonnam National University) ;
  • Kim, Hyun-Seung (RIS Foundation for Advanced Biomaterials, Chonnam National University) ;
  • Lim, Hyun-Pil (Department of Prosthodontics, School of Dentistry, Chonnam National University)
  • 투고 : 2014.09.17
  • 심사 : 2014.12.23
  • 발행 : 2015.04.30
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초록

PURPOSE. The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system. MATERIALS AND METHODS. Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction. RESULTS. The number of S. mutans colonies on the TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05). CONCLUSION. The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

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