한국패류학회지 (The Korean Journal of Malacology)

한국패류학회 (The Malacological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

수온 상승에 따른 북방전복, Haliotis discus hannai과 둥근전복, H. discus discus 체액의 생리학적 변화

Influence of Elevated Temperatures on the Physiological Response of Hemolymph from Two Species of the Abalone, Haliotis discus hannai and H. discus discus

  • 민은영 (부경대학교 수산과학연구소) ;
  • 이정식 (전남대학교 수산생명의학과) ;
  • 김재원 (강원도립대학 해양생명과학과) ;
  • 전미애 (전남대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Min, Eun-Young (Institute of Fisheries Science, Pukyong National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jae Won (Department of Marine Life-Science, Gangwon Provincial College) ;
  • Jeon, Mi Ae (Department of Aqualife Medicine, Chonnam National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2015.02.13
  • 심사 : 2015.03.30
  • 발행 : 2015.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 수온 증가에 따른 북방전복 (Haliotis discus hannai) 과 둥근전복 (H. discus discus) hemolymph의 생리 및 면역학적 변화를 관찰하기 위하여 위 두 종을 20, 22, 24, 26 및 $28^{\circ}C$ 수온에 각각 4 일간 노출시켰다. 노출 결과, hemolymph의 total-protein (TP), glucose, 칼슘 (Ca) 은 둥근전복이 북방전복보다 높은 값을 보였으나, 마그네슘 (Mg), alkaline phosphatase (ALP) 및 lysozyme은 두 종에서 유사한 값을 보였다. 수온 증가에 따른 hemolymph의 TP, glucose 및 마그네슘은 두 전복 모두에서 유의한 변화가 관찰되지 않았다. 그러나 칼슘과 면역관련 인자인 ALP 및 lysozyme은 둥근전복에서 유의한 차이가 없었으나, 북방전복에서는 $26^{\circ}C$ 또는 $28^{\circ}C$의 고수온에 노출되었을 때, 유의하게 높은 활성을 보였다. 한편 phenoloxidase (PO)는 북방전복에서 높은 값을 보였으며, 수온이 높을수록 증가하는 경향을 보였다. 결론적으로 북방전복은 둥근전복과 달리 여러 지표에서 유의한 변화가 관찰된 것으로 보아 북방전복이 둥근전복보다 고수온에 보다 민감한 것으로 사료된다.

This study was conducted to investigate the effects of elevated water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis discus hannai and H. discus discus. The abalone were exposed to various WT; 20, 22, 24, 26 and $28^{\circ}C$ for 4 days. In the control and $20^{\circ}C$, total-protein (TP), glucose and calcium (Ca) in hemolymph of H. discus discus were higher than the values in H. discus hannai. The values of magnesium (Mg), alkaline phosphatase (ALP) and lysozyme in H. discus hannai were similar to the H. discus discus in the control. There were no significant alterations in TP, glucose and Mg levels of hemolymph in H. discus hannai and H. discus discus by WT increases. The values of Ca, ALP and lysozyme were increased in H. discus hannai exposed to the high temperature (26 and $28^{\circ}C$) compared to control, while the values in H. discus discus were not significant difference between the WT groups. The phenoloxidase (PO) activity was increased in hemolymph of H. discus hannai exposed to high temperature (${\geq}24^{\circ}C$) compared to the control (P < 0.05). These physiological and immunological parameters were significantly changed in H. discus hannai. However, these parameters in H. discus discus were barely altered at the high WT (P < 0.05). These results suggested that H. discus hannai is considered to be more sensitive than H. discus discus at the high WT.

키워드

참고문헌

  1. Anderson, R.S., Giam, C.S., Ray, L.E. and Tripp, M.R. (1981) Effects of environmental pollutants on immunological competency in the clam, Mercenaria mercenaria: impacted bacterial clearance. Aquatic Toxicology, 1: 187-195. https://doi.org/10.1016/0166-445X(81)90014-X
  2. Ashida, M. and Yamazaki, H.I. (1990) Biochemistry of the phenoloxidase system in insets: with special reference to its activation. In: Molting and metamorohosis. (ed. by Ohnishi, E. and Ishizaki, H.) Springer, Berlin. pp. 239-265.
  3. Asokan, R., Arumugam, M. and Mullainadha, P. (1997) Activation of prophenoloxidase in the plasma and hemocytes of the marine mussel Perna viridis Linnaeus. Developmental and Comparative Immunology, 21: 1-12. https://doi.org/10.1016/S0145-305X(97)00004-9
  4. Baeck, S.K., Min, E.Y. and Kang, J.C. (2014) Combined effects of copper and temperature on hematological constituents in the rock fish, Sebastes schlegeli. Journal of Fish Pathology, 27(1): 57-65. https://doi.org/10.7847/jfp.2014.27.1.057
  5. Barton, B.A. (1991) Physiological changes in fish from stress in aquaculture with emphasis in the response and effects of corticosteroids. Annual Review of Fish diseases, 1: 3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  6. Byrne, R.A., Shipman, B.N., Smatrean, N.J., Dietz, T.H. and McMahon, R.F. (1991) Acid-base balance during emergence in the freshwater bivalve Corbicula fluminea. Physiological Zoology, 64: 748-766. https://doi.org/10.1086/physzool.64.3.30158205
  7. Chang, Y.J., Hur, J.W., Kim, H.K. and Lee, J.K. (2001) Stress in olive flounder (Paralichthys olivaceus) and fat cod (Hexagrammos otakii) by the sudden drop and rise of water temperature. Journal of the Korean Fisheries Society, 34(2): 91-97.
  8. Cheng, T.C. (1981) Bivalves. In: Invertebrate blood cell (ed. by Ratcliffe, N.A. and Rowley, A.F.), London Academic Press, London. pp. 233-299.
  9. Cheng, W., Li, C.H. and Chen, J.C. (2004) Effect of dissolved oxygen on the immune responses of Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Aquaculture, 232: 103-115. https://doi.org/10.1016/S0044-8486(03)00488-5
  10. Day, R., Hooper, C., Benkendorff, K., Slocombe, R. and Handlinger, J. (2010) Investigation on the immunology of stressed abalone. Final report of FRDC project number: 2004/233. University of Melbourne. 1-89.
  11. Eune, J.J., Lee, E.S., Rim, J.S., Jang, H.S. and Woo, H.I. (2005) Changes of serum alkaline phosphatase after enucleation of systs in the jaws. Journal of the Korean Association of Oral and Maxillofacial Surgeons, 31: 417-421.
  12. Feng, S.Y. and Canzonier, W.J. (1970) Humoral responses in the American oyster (Crssostrea virginica) infected with Bucephalus sp. and Minchinia nelsoni. American Fisheries Society Special Publication, 5: 497-510.
  13. Firat, O., Cogun, H.Y., Aslanyavrusu, S. and Kargin, F. (2009) Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposure. Journal of Applied Toxicology, 29: 295-301. https://doi.org/10.1002/jat.1406
  14. Gopalakrishnan, S., Huang, W.B., Wang, Q.W., Wu, M.L., Liu, J. and Wang, K.J. (2011) Effect of tributyltin and benzo(a)pyrene on the immune-associated activities of hemocytes and recovery responses in the gastropod abalone, Haliotis diversicolor. Comparative Biochemistry and Physiology, 154(C): 120-128.
  15. Hagerman, L. and Szaniawska, A. (1991) Ion regulation under anoxia in the brackish water isopod Saduria (Mesiidotea) entomon. Ophelia, 33: 97-104. https://doi.org/10.1080/00785326.1991.10429732
  16. Hontela, A., Daniel, C. and Ricard, A.C. (1996) Effects of acute and subacute exposures to cadmium on the interrenal and thyroid function in rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology, 35: 171-182. https://doi.org/10.1016/0166-445X(96)00012-4
  17. Hur, J.W., Chang, Y.J., Lim, H.K. and Lee, B.K. (2001) Stress responses of cultured fishes elicited by water level reduction in rearing tank and fish transference during selection process. Bulletin of the Korean Fisheries Society, 34: 465-472.
  18. Hutchinson, T.M. and Manning, M.J. (1996) Seasonal trends in serum lysozyme activity and total protein concentration in dab (Limanda limanda L.) sampled from Lyme Bay, U.K.. Fish & Shellfish Immunology, 6: 473-482. https://doi.org/10.1006/fsim.1996.0045
  19. Jang, M.S., Jang, J.R., Park, H.Y. and Yoon, H.d. (2010) Overall composition, and levels of fatty acids, amino acids, and necleotide-type compounds in wild abalone Haliotis gigantea and cultured abalone Halitotis discus hannai. Korean Journal of Food Preservation, 17: 533-540.
  20. Jee, J.H., Kim, S.G. and Kang, J.C. (2004) Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Paralichthys olivaceus. Journal of Experimental Marine Biology and Ecology, 304: 123-136. https://doi.org/10.1016/j.jembe.2003.12.001
  21. Johnson, J.K., Rocheleau, T.A., Hillyer, J.F., Chen, C.C., Li, J. and Christensen, B.M. (2003) A potential role for phenylalanine hydroxylase in mosquito immune responses. Insect Biochemistry and Molecular Biology, 33: 345-354. https://doi.org/10.1016/S0965-1748(02)00257-6
  22. Kim, T.H., Yang, M.H., Choe, M.K., Han, S.J. and Yeu, I.K. (2005) Physiological studies on acute water-temperature stress of juvenile abalone, Haliotis discus hannai. Journal of Aquaculture, 18: 7-12.
  23. Kim, T.H., Kim, K.J., Choe, M.K. and Yeo, I.K. (2006) Physiological changes of juvenile abalone, Haliotis sieboldii exposed to acute water temperature stress. Journal of Aquaculture, 19: 77-83.
  24. KNSO (2011) Korea National Statistical office. Fishery production survey DB, Daejeon, Korea.
  25. Liang, S., Luo, X., You, W., Luo, L., Liu, C.K., Zhang, J.F., Shen, H., Wang, X.R. and Wang, W.M. (2014) The role of hybridization in improving the immnune response and thermal tolerance of abalone. Fish and Shellfish Immunology, 39: 69-77. https://doi.org/10.1016/j.fsi.2014.04.014
  26. Lin, A. and Meyers, M.A. (2005) Growth and structure in abalone shell. Materials Science and Engineering, 390(A): 27-41. https://doi.org/10.1016/j.msea.2004.06.072
  27. Martello, L. and Tjeerdema, R.S. (2001) Combined effects of pentachorophenol and salinity stress on chemiluminescence activity in two species of abalone. Aquatic Toxicology, 51:351-362. https://doi.org/10.1016/S0166-445X(00)00110-7
  28. Min, E.Y., Jeong, J.W. and Kang, J.C. (2014a) Thermal effects on antioxidant enzymes responses in Tilapia, Oreochromis niloticus exposed Arsenic. Journal of Fish Pathology., 27(2): 115-125. https://doi.org/10.7847/jfp.2014.27.2.115
  29. Min, E.Y., Baeck, S.K. and Kang, J.C. (2014b) Combined effects of copper and temperature on antioxidant enzymes in the black rockfish Sebastes schlegeli. Fisheries and Aquatic Sciences, 17(3): 345-353. https://doi.org/10.5657/FAS.2014.0345
  30. Morris, S. and Butler, S.L. (1996) Hemolymph respiratory gas, acid-base, and ion status of the amphibious purple shore crab Leptograpsus variegates (Fabricus) during immersion and environmental hypoxia. Journal of Crustacean Biology, 16: 253-266. https://doi.org/10.1163/193724096X00045
  31. Nikinmaa, M., Jaervenpaeae, T., Westman, K. and Soivio, A. (1985) Effects of hypoxia and acidification on the haemolymph pH value and ion concentration in the freshwater crayfish (Astacus astacus L.). Finnish Fisheries Ressearch, 5: 17-22.
  32. Park, C.J., Min, B.H., Kim, K.S., Lee, J.W., Lee, J.H., Noh, J.K., Kim, H.C., Park, J.W. and Myeong, J.I. (2011) Physiological responses on low water temperature stress of Pacific abalone, Haliotis discus hannai. Korean Journal of Malacology, 27: 317-322. https://doi.org/10.9710/kjm.2011.27.4.317
  33. Park, H.J. and Kang, J.C. (2012) Biochemical changes in the hemolymph and hepatopancreas of abalone Haliotis discus hannai exposed to copper. Korean Journal of Fisheries and Aquatic Sciences, 45: 154-160. https://doi.org/10.5657/KFAS.2012.0154
  34. Park, M.W., Kim, H., Kim, B.H., Son, M.H., Jeon, M.A. and Lee, J.S. (2013) Changes of survival rate, falling rate and foot histology of the abalone, Haliotis discus hannai (Ino, 1952) with water temperature and salinity. Korean Journal of Malacology, 29(4): 303-311. https://doi.org/10.9710/kjm.2013.29.4.303
  35. Ryan, S.N. (1995) The effect of chronic heat stress on cortisol levels in the Antartic fish Pagothenia borchgrevinki. Experimentia, 51: 768-774. https://doi.org/10.1007/BF01922428
  36. Seong, K.T., Hwang, J.D., Han, I.S., Go, W.J., Suh, Y.S. and Lee, J.Y. (2010) Characteristic for long-term trends of temperature in the Koreans waters. Journal of the Korean Society of Marine Environment and Safety, 16: 353-360.
  37. Shen, H., Zhang, Q., Xu, R. and Wang, G. (1997) Effects of petroleum on the sero-proteins of Tilapia mossambica. Marine Environmental Science, 16: 1-5.
  38. Shin, Y.K., Jun, J.C., Im, J.H., Kim, D.W., Son, M.H. and Kim, E.O. (2011) Physiological response in abalone Haliotis discus hannai with different salinity. Korean Journal of Malacology, 27: 283-289. https://doi.org/10.9710/kjm.2011.27.4.283
  39. Shin, Y.K., Lee, W.C., Kim, D.W., Son, M.H., Jun, J.C., Kim, E.O. and Kim, S.H. (2012) Seasonal changes in physiology of the abalone Haliotis discus hannai reared from Nohwa Island on the south coast of Korea. Korean Journal of Malacology, 28: 131-136. https://doi.org/10.9710/kjm.2012.28.2.131
  40. Siiderhall, K., Cerenius, L. and Johansson, M.W. (1994) The prophenoloxidase activating system and its role in invertebrate defense. Primordial immunity: foundations for the vertebrate immune system. Annals of the New York Academy of Sciences, 712: 155-161. https://doi.org/10.1111/j.1749-6632.1994.tb33570.x
  41. Stohs, S.J., Bagchi, D., Hassoun, E. and Bagchi, M. (2000) Oxidative mechanism in the toxicity of chromium and cadmium ions. Journal of environmental Pathology Toxicology and Oncology, 19: 201-213.
  42. Wang, S.H., Wang, Y.L., Zhang, Z.X., Jack, R., Weng, Z.H., Zou, Z.H. and Zhang, Z.P. (2004). Response of innate immune factors in abalone Haliotis diversicolor supertexta to pathogenic or nonpathogenic infection. Journal of Shellfish Research, 23: 1173-1178.
  43. Waring, C.P., Stagg, R.M. and Roxton, M.G. (1996) Physiological responses to handling in the turbot. The Journal of Fish Biology, 48: 161-173. https://doi.org/10.1111/j.1095-8649.1996.tb01110.x
  44. Widdows, J. (1985) The effects of fluctuating and abrupt changes in salinity on the performance of Mytilus edulis. In: Marine Biology of Polar Regions and Effects of Stress on Marine Organism. (ed. by Gray, J.S., Christiansen, M.E.), Wiley-Interscience, New York. pp. 555-566.
  45. Yang, J.W., Lee, J.S., Han, I.S., Choi, Y.K. and Suh, Y.S. (2012) Seawater temperature variation of aquafarms off Wando in the southwest coast of Korea. Journal of the Korean Society of Marine Environment and Safety, 18: 514-519. https://doi.org/10.7837/kosomes.2012.18.6.514
  46. Yoo, S.K. (1986) Coastal culture, Gudeok publisher, p. 359.
  47. Zaccaron da Silva, A., Zanette J., Ferrira J.F., Guzenski, J., Marques, M.R.F. and Bainy, A.C.D. (2005) Effect of salinity on biomarker responses in Crassostrea rhizophorae (Mollusca, Bivalvia) exposed to diesel oil. Ecotoxicology and Environmental Safety, 62: 376-382. https://doi.org/10.1016/j.ecoenv.2004.12.008

피인용 문헌

  1. Influence of elevated temperatures on the physiological response of hemolymph from two species of abalone, Haliotis gigantea and Haliotis discus discus (Reeve, 1846) vol.31, pp.3, 2015, https://doi.org/10.9710/kjm.2015.31.3.187