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Optimization of Ultraviolet Irradiate Conditions for Vitamin D2 Synthesis in Shitake Mushrooms (Lentinula edodes) by Using Response Surface Methodology

  • Zhang, Ya (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Wu, Wei-Jie (Department of Oriental Medicine Resources, Chonbuk National University) ;
  • Song, Geun-Seoup (Department of Food Science and Technology, Chonbuk National University) ;
  • Ahn, Byung-Yong (Department of Oriental Medicine Resources, Chonbuk National University)
  • Received : 2014.07.31
  • Accepted : 2014.10.01
  • Published : 2015.03.31

Abstract

The determination of the optimum conditions for the conversion of ergosterol to vitamin $D_2$ in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature ($20-40^{\circ}C$), exposure time (60-180 min), and irradiation intensity ($0.6-1.8W/m^2$) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of $34.2^{\circ}C$, exposure time of 175.6 min, and irradiation intensity of $1.41W/m^2$. Under these optimum conditions, the maximum vitamin $D_2$ content of $117.93{\mu}g/g$ in shitake mushrooms was obtained, which agreed fairly well with the predicted value of $122.60{\mu}g/g$.

Keywords

References

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