Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genetic Effects of Polymorphisms in Myogenic Regulatory Factors on Chicken Muscle Fiber Traits

  • Yang, Zhi-Qin (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Qing, Ying (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Zhu, Qing (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Zhao, Xiao-Ling (College of Animal Science and Technology, Sichuan Agricultural University) ;
  • Wang, Yan (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Li, Di-Yan (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Liu, Yi-Ping (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Yin, Hua-Dong (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University)
  • Received : 2014.09.25
  • Accepted : 2015.01.12
  • Published : 2015.06.01
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Abstract

The myogenic regulatory factors is a family of transcription factors that play a key role in the development of skeletal muscle fibers, which are the main factors to affect the meat taste and texture. In the present study, we performed candidate gene analysis to identify single-nucleotide polymorphisms in the MyoD, Myf5, MyoG, and Mrf4 genes using polymerase chain reaction-single strand conformation polymorphism in 360 Erlang Mountain Chickens from three different housing systems (cage, pen, and free-range). The general linear model procedure was used to estimate the statistical significance of association between combined genotypes and muscle fiber traits of chickens. Two polymorphisms (g.39928301T>G and g.11579368C>T) were detected in the Mrf4 and MyoD gene, respectively. The diameters of thigh and pectoralis muscle fibers were higher in birds with the combined genotypes of GG-TT and TTCT (p<0.05). Moreover, the interaction between housing system and combined genotypes has no significant effect on the traits of muscle fiber (p>0.05). Our findings suggest that the combined genotypes of TT-CT and GG-TT might be advantageous for muscle fiber traits, and could be the potential genetic markers for breeding program in Erlang Mountain Chickens.

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References

  1. Branciari, R., C. Mugnai, R. Mammoli, D. Miraglia, D. Ranucci, A. Dal Bosco, and C. Castellini. 2009. Effect of genotype and rearing system on chicken behavior and muscle fiber characteristics. J. Anim. Sci. 87:4109-4117. https://doi.org/10.2527/jas.2009-2090
  2. Braun, T., E. Bober, B. Winter, N. Rosenthal, and H. H. Arnold. 1990. Myf-6, a new member of the human gene family of myogenic determination factors: Evidence for a gene cluster on chromosome 12. EMBO. J. 9:821-831.
  3. Braun, T., G. Buschhausen-Denker, E. Bober, E. Tannich, and H. H. Arnold. 1989. A novel human muscle factor related to but distinct from myod1 induces myogenic conversion in 10t1/2 fibroblasts. EMBO. J. 8:701-709.
  4. Davis, R. L., H. Weintraub, and A. B. Lassar. 1987. Expression of a single transfected cdna converts fibroblasts to myoblasts. Cell 51:987-1000. https://doi.org/10.1016/0092-8674(87)90585-X
  5. Dou, T. C., S. Shi, R. Sun, H. J. Wang, H. J. Sun, and K. H. Wang. 2009. Growth rate, carcass traits and meat quality of slowgrowing chicken grown according to three raising systems. Anim. Sci. Papers. Rep. 27:361-369.
  6. Edmondson, D. G. and E. N. Olson. 1989. A gene with homology to the myc similarity region of myod1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. Genes Dev. 3:628-640. https://doi.org/10.1101/gad.3.5.628
  7. Fanatico, A. C., P. B. Pillai, P. Y. Hester, C. Falcone, J. A. Mench, C. M. Owens, and J. L. Emmert. 2008. Performance, livability, and carcass yield of slow- and fast-growing chicken genotypes fed low-nutrient or standard diets and raised indoors or with outdoor access. Poult. Sci. 87:1012-1021. https://doi.org/10.3382/ps.2006-00424
  8. Kimchi-Sarfaty, C., J. M. Oh, I.-W. Kim, Z. E. Sauna, A. M. Calcagno, S. V. Ambudkar, and M. M. Gottesman. 2007. A "silent" polymorphism in the mdr1 gene changes substrate specificity. Science 315(5811):525-528. https://doi.org/10.1126/science.1135308
  9. Kolmodin, R., E. Strandberg, H. Jorjani, and B. Danell. 2003. Selection in the presence of a genotype by environment interaction: Response in environmental sensitivity. Anim. Sci. 76:375-386. https://doi.org/10.1017/S1357729800058604
  10. Komar, A. A. 2007. Silent snps: Impact on gene function and phenotype. Pharmacogenomics 8:1075-1080. https://doi.org/10.2217/14622416.8.8.1075
  11. Massari, M. E. and C. Murre. 2000. Helix-loop-helix proteins: Regulators of transcription in eucaryotic organisms. Mol. Cell. Biol. 20:429-440. https://doi.org/10.1128/MCB.20.2.429-440.2000
  12. Orita, M., Y. Suzuki, T. Sekiya, and K. Hayashi. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874-879. https://doi.org/10.1016/0888-7543(89)90129-8
  13. Sing, C. F. 1990. Introduction to quantitative genetics. Am. J. Hum. Gent. 46(6):1231.
  14. Singh, K. and F. J. Dilworth. 2013. Differential modulation of cell cycle progression distinguishes members of the myogenic regulatory factor family of transcription factors. FEBS. J. 280: 3991-4003. https://doi.org/10.1111/febs.12188
  15. Wang, K. H., S. R. Shi, T. C. Dou, and H. J. Sun. 2009. Effect of a free-range raising system on growth performance, carcass yield, and meat quality of slow-growing chicken. Poult. Sci. 88:2219-2223. https://doi.org/10.3382/ps.2008-00423
  16. Weintraub, H., R. Davis, S. Tapscott, M. Thayer, M. Krause, R. Benezra, T. K. Blackwell, D. Turner, R. Rupp, and S. Hollenberg. 1991. The myod gene family: Nodal point during specification of the muscle cell lineage. Science 251(4995): 761-766. https://doi.org/10.1126/science.1846704
  17. Yin, H. D., Y. Wang, Z. C. Zhang, Y. P. Liu, S. Y. Chen, and Q. Zhu. 2013. Characterization of the expression profile and genetic polymorphism of the cellular retinol-binding protein (crbp iv) gene in erlang mountainous chickens. Int. J. Mol. Sci. 14:4432-4443. https://doi.org/10.3390/ijms14034432
  18. Yin, H. D., E. R. Gilbert, S. Y. Chen, Y. Wang, Z. C. Zhang, X. L. Zhao, Y. Zhang, and Q. Zhu. 2013. Effect of hybridization on carcass traits and meat quality of erlang mountainous chickens. Asian Australas. J. Anim. Sci. 26:1504-1510. https://doi.org/10.5713/ajas.2013.13097
  19. Yin, H., Z. Zhang, X. Lan, X. Zhao, Y. Wang, and Q. Zhu. 2011. Association of myf5, myf6 and myoG gene polymorphisms with carcass traits in chinese meat type quality chicken populations. J. Anim. Vet. Adv. 10:704-708. https://doi.org/10.3923/javaa.2011.704.708

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