생명과학회지 (Journal of Life Science)

  • 제25권11호
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  • Pages.1311-1318
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  • 2015
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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수확시기별 제주산 영귤의 항산화 및 항당뇨 활성 비교

Changes in Phenolic Composition, Antioxidant and Antidiabetic Properties of Jeju Citrus sudachi as Influenced by Maturity

  • 이지은 (제주대학교 생명자원과학대학 바이오소재전공 독성학 실험실) ;
  • 김지혜 (제주대학교 생명자원과학대학 바이오소재전공 독성학 실험실) ;
  • 김민영 (제주대학교 생명자원과학대학 바이오소재전공 독성학 실험실)
  • Lee, Ji Eun (Toxicology Laboratory, Major in Biomaterials, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Ji Hye (Toxicology Laboratory, Major in Biomaterials, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Min Young (Toxicology Laboratory, Major in Biomaterials, College of Applied Life Science, SARI, Jeju National University)
  • 투고 : 2015.08.24
  • 심사 : 2015.11.17
  • 발행 : 2015.11.30
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초록

본 연구는 9월과 11월에 수확한 영귤 미성숙과와 성숙과의 항산화 및 항당뇨 활성을 비교하였다. 총 폴리페놀, 플라보노이드 및 카로티노이드 함량을 분석한 결과 모두 성숙과의 함량이 높았다. 항산화 활성을 DPPH radical, hydrogen peroxide, nitric oxide 소거능, 금속 결합능 및 환원력으로 평가하고 이를 EC50으로 나타낸 결과 금속 결합능을 제외하고 성숙과의 활성이 유의적으로 높았다. α-Glucosidase 저해 활성을 통해 항당뇨 활성을 확인한 결과 성숙과가 높은 저해도를 나타내었다. 이를 통해 출하 시기를 놓친 영귤 성숙과도 건강기능식품 및 의약품 소재로 개발 가능성이 있다고 판단되며 이를 활용하기 위한 추가적인 연구가 필요하다고 사료된다.

The effects of fruit maturation on changes in the total phenolics, flavonoids, and carotenoids of methanolic extracts of Citrus sudachi, in addition to its antioxidant and antidiabetic activities, were determined. Generally, the concentration of these chemical constituents increased as C. sudachi reached maturity. C. sudachi contained high levels of total phenolics, flavonoids, and carotenoids at maturity, contributing 6339.5 mg of gallic acid equivalent per 100 g, 2364.2 mg of rutin equivalent per 100 g, and 678.7 mg/ml, respectively. The scavenging activities of 1,1-diphenyl-2-picryl hydrazyl (DPPH), hydrogen peroxide and nitric oxide radicals and the reducing power of mature fruits were significantly higher at all data points than those of immature fruits (p<0.05). In contrast, the ferrous ion chelating activity of mature and immature C. sudachi fruits was similar. The 50% effective concentrations (EC50) of mature fruits were 4.1±0.10 mg/ml for scavenging DPPH radicals, 3.1±0.02 mg/ml for scavenging hydrogen peroxide, 3.9±0.01 mg/ml for scavenging nitric oxide, and 3.8±0.02 mg/ml for chelating ferrous ion. The antidiabetic activity of C. sudachi was studied in vitro using the α-glucosidase inhibitory method. The inhibitory activity of mature C. sudachi fruits on α-glucosidase was higher than that of immature fruits. These results suggest that the content of bioactive compounds and the antioxidant and antidiabetic activities of C. sudachi change during maturation. These findings can be further extended to exploit them for their possible application for the preservation of food products, as well as their use as health supplements and nutraceuticals.

키워드

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피인용 문헌

  1. Engl.) during fermentation vol.60, pp.3, 2017, https://doi.org/10.3839/jabc.2017.037
  2. (Thunb.) Engl.) extracts vol.60, pp.3, 2017, https://doi.org/10.3839/jabc.2017.038