생명과학회지 (Journal of Life Science)

  • 제25권11호
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  • Pages.1273-1279
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  • 2015
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양성 Simiduia sp. SH-1 균주의 분리 및 한천분해효소의 특성조사

Characterization of Agarase from an Isolated Marine Bacterium, Simiduia sp. SH-1

  • 이솔지 (신라대학교 일반대학원 바이오과학과) ;
  • 오수정 (신라대학교 제약공학과) ;
  • 이동근 (신라대학교 일반대학원 바이오과학과) ;
  • 이상현 (신라대학교 일반대학원 바이오과학과)
  • Lee, Sol-Ji (Department of Bioscience, Graduate School, Silla University) ;
  • Oh, Soo-Jeong (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience, Graduate School, Silla University) ;
  • Lee, Sang-Hyeon (Department of Bioscience, Graduate School, Silla University)
  • 투고 : 2015.07.23
  • 심사 : 2015.09.20
  • 발행 : 2015.11.30
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초록

경상남도 남해군 연안의 해수를 이용하여 신규의 한천분해 해양성세균을 분리하고 한천분해효소의 특징을 분석하였다. Marine agar 2216 배지에서 분리한 SH-1 균주는 16S rRNA 유전자 염기서열분석을 통해 Simiduia 속 세균과 99% 유사하여 Simiduia sp. SH-1으로 명명하였다. Simiduia sp. SH-1 균주가 생성하는 한천분해효소는 성장의존성 산물로 판단되었으며 정체기부터 효소활성이 감소되었다. 한천분해효소는 pH 7.0(20 mM Tris-HCl buffer), 30℃에서 최대활성(698.6 units/l)을 나타내었다. 효소의 활성은 30℃에서 최적이었고 이후 온도가 증가함에 따라 활성이 감소하였으며, 40℃와 50℃에서 각각 약 90%와 75%의 상대활성을 보였으나 내열성은 보이지 않았다. 최적 pH인 pH 7.0에 비해 pH 6.0에서는 오차범위 내에서 조금 낮은 활성을 보였으며 pH 5.0과 pH 8.0에서는 각각 80%와 75% 정도의 상대활성을 보였다. TLC 분석을 통하여 Simiduia sp. SH-1 균주가 생성하는 한천분해효소는 agarose를 분해하여 피부의 미백활성, 전분노화의 방지 및 세균성장의 억제 등의 유용한 효과를 가지는 기능성 한천올리고당인 neoagarotetraose와 neoagarobiose를 최종적으로 생성하는 β-agarase로 확인되었다. 따라서, Simiduia sp. SH-1 균주와 이 균주가 생산하는 β-agarase는 산업적 생산에 유용하게 활용될 수 있을 것으로 기대된다.

Agarase from a novel agar-degrading bacterium isolated from seawater in Namhae at Gyeongsangnamdo province of Korea was characterized. The SH-1 strain was selected from thousands of colonies on Marine agar 2216 media. Almost full 16S rRNA gene sequence of the agarolytic SH-1 strain showed 99% similarity with that of bacteria of Simiduia genus and named as Simiduia sp. SH-1. Agarase production was growth related, and activity was declined from stationary phase. Secreted agarase was prepared from culture media and characterized. It showed maximum activity of 698.6 units/L at pH 7.0 and 30℃ in 20 mM Tris-HCl buffer. Agarase activity decreased as the temperature increased from an optimum of 30℃, with 90% and 75% activity at 40℃ and 50℃, respectively. Agarase was not heat resistant. Slightly lower agarase activity was observed at pH 6.0 than at pH 7.0, without statistical difference, and 80% and 75% activity were observed at pH 5.0 and 8.0, respectively. Neoagarotetraose and neoagarobiose were the main final products of agarose, indicating that it is β-agarase. Simiduia sp. SH-1 and its β-agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose, which have a whitening effect on skin, delaying starch degradation, and inhibiting bacterial growth.

키워드

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