생명과학회지 (Journal of Life Science)

  • 제25권11호
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  • Pages.1265-1272
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  • 2015
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

식용피 밀양3호[Echinochloa esculenta (A. Braun)] 에탄올 추출물의 당뇨모델 마우스에 대 한 항당뇨 활성

Anti-diabetic Effects of Barnyard Millet Miryang 3 [Echinochloa esculenta (A. Braun)] Grains on Blood Glucose in C57BL/KsJ-db/db Mice

  • 권기현 (경북대학교 자연과학대학 생명과학부) ;
  • 전도연 (경북대학교 자연과학대학 생명과학부) ;
  • 이지영 (경북대학교 자연과학대학 생명과학부) ;
  • 박주은 (경북대학교 자연과학대학 생명과학부) ;
  • 우미희 (대구가톨릭대학교 약학대학) ;
  • 윤영호 (국립식량과학원 남부작물부) ;
  • 고지연 (국립식량과학원 남부작물부) ;
  • 오인석 (국립식량과학원 남부작물부) ;
  • 김영호 (경북대학교 자연과학대학 생명과학부)
  • Kwon, Gi Hyun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Jun, Do Youn (Institute of Life Science and Biotechnology, Kyungpook National University) ;
  • Lee, Ji Young (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Jueun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Woo, Mi Hee (Department of Pharmacology, College of Pharmacology, Daegu Catholic University) ;
  • Yoon, Young Ho (Upland Crop Breeding Research DIV, Department of Southern Area Crop Science, RDA) ;
  • Ko, Jee Youn (Upland Crop Breeding Research DIV, Department of Southern Area Crop Science, RDA) ;
  • Oh, In-Seok (Upland Crop Breeding Research DIV, Department of Southern Area Crop Science, RDA) ;
  • Kim, Young Ho (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • 투고 : 2015.11.24
  • 심사 : 2015.11.25
  • 발행 : 2015.11.30
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초록

잡곡의 하나인 식용피는 폴리페놀과 수용성 식이섬유성분이 풍부하고 전분함량이 타 곡류보다 낮으면서 단백질 함양이 높아 영양학적 건강지표가 높은 잡곡이다. 본 연구에서는 항염증 활성이 강하게 확인된 밀양3호를 선발하여 이의 항당뇨 활성에 관해 조사하였다. 식용피 밀양3호의 80% 에탄올(EtOH) 추출물을 당뇨모델인 C57BL/KsJ-db/db mice에 600 mg/kg로 8주간 투여하여 혈당과 혈중 지질에 대한 영향을 조사한 결과, 공복혈당이 당뇨 대조군에 비해 유의적으로 감소하여 개선 되었으며, 경구 내당성도 1주 투여 시부터 개선되는 효과를 보이다가 8주간의 투여 시는 양성대조군인(메트포민 100 mg/kg) 수준으로 경구 내당성이 개선됨을 확인하였다. 이때 체중증가는 메트포민 투여군(10.36±3.15)에는 못 미치나 8.54±2.24로 당뇨 대조군 체중 증가(8.71±1.64)의 수준이었으며, food efficiency rates를 고려하면 식용피가 식이 섭취에는 큰 영향을 주지 않는 것으로 사료되었다. 총 콜레스테롤 농도는 당뇨 대조군의 총콜레스테롤(229.3±47.8)에 비해 식용피 EtOH 추출물 투여군(160.7±7.6)과 메트포민 투여군(176.0±25.6)에서 유의적으로 감소하였다. LDL-콜레스테롤 농도는 식용피 투여군에서 메트포민 투여군 수준으로 당뇨 대조군에 비해 약 41%정도 감소한 것에 반해, 중성지방은 식용피 추출물의 영향을 받지 않았다. 간독성은 식용피 추출물군의 GOT 값이 메트포민 투여군 보다 더 낮았으며, GPT 활성도는 모든 실험군들 간에 유의한 차이가 없었다. 이상의 연구 결과는 식용피 밀양3호가 항 당뇨 관련 우수한 소재 함유 곡류로 관련 질환 예방과 치료에 활용할 수 있음을 시사한다.

Barnyard millet Miryang 3 [Echinochloa esculenta (A. Braun)] grains have recently been acknowledged for beneficial health properties due to phenolic ingredients and dietary fiber. This study has been conducted on the anti-diabetic activity of barnyard millet Miryang 3 which shows the strongest anti-inflammatory activity among barnyard millet inhabiting in South Korea. When 80% ethanol (EtOH) extract of barnyard millet Miryang 3 grains were orally administered into db/db diabetic mice for 8 weeks (600 mg/kg/day), the glucose level in blood following fasting appeared to be improved compared to the control group. The results of glucose tolerance test and blood lipid profile assay were similar to those of the metformin-administered positive control group. In addition, the level of body weight increase (8.54±2.24) was lower than the level of metformin-administered group (10.36±3.15); however, there was no subtle difference with negative and positive control groups in terms of food efficiency rates. In addition, total cholesterol levels of the 80% EtOH extract-administered group (160.7±7.6) were significantly reduced compared to the diabetic control group (229.3±47.8) and metformin-administered group (176.0±25.6). Consequently, these results show that barnyard millet grains alleviates many of the diabetic symptoms in vivo non-insulin-dependent diabetes mellitus, and suggest that barnyard millet grains can be applicable in developing new functional food materials.

키워드

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