Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Immune Responses of BALB/c Mice Administrated via Oral Route to a Combined Salmonella Typhimurium Ghost Vaccine

복합 살모넬라 타이피무리움 고스트 백신의 마우스 구강 투여에 의한 면역 응답

  • Kim, Pan Gil (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Ha, Yeon Jo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Lee, Su Man (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Sam Woong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김판길 (경남과학기술대학교 제약공학과) ;
  • 하연조 (경남과학기술대학교 제약공학과) ;
  • 이수만 (경남과학기술대학교 제약공학과) ;
  • 김삼웅 (경남과학기술대학교 제약공학과) ;
  • 갈상완 (경남과학기술대학교 제약공학과)
  • Received : 2015.06.15
  • Accepted : 2015.10.26
  • Published : 2015.11.30
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Abstract

Salmonella Typhimurium (ST) JOL389 and χ3339 are strong virulent strains against mouse. ST χ8554 is derived by deletion of the asd gene from ST χ3339. Plasmid pMMP184 carrying a ghost cassette was transformed into ST χ8554, and ST χ8554 ghost cells were prepared and administrated via the oral route to BALB/c mice. Change in the amount of total IgG was not elicited to boosting of single ST χ8554 ghost cells, but the content was increased from 6 weeks after the 3rd administration. However, when the ST JOL389 ghost cells is administered together with ST χ8554 ghost cells, the content of total IgG was increased in 2 weeks post primary administration. It was found that the content of total IgG of the group mixed with ST JOL389 ghost cells showed an increased value of 8 times or more at 10 weeks when compared with the group of ST χ8554 ghost cells. The content of IgG1, IgG2a, and sIgA in both groups increased from 4 weeks postprimary administration. As a challenge test of virulent ST χ3339, χ8554 (pMMP184) and χ8554 (pMMP184)/JOL389 ghost cell groups showed protection of 50% or more when compared to the control group. These results suggest that the preparation of combined ghost cells from a strong virulent ST increases immunity more than a single strain.

살모넬라 타이피무리움 JOL389와 χ3339는 마우스에 강한 독력을 가진 균주들이며, χ8554는 χ3339로부터 유도되었다. 고스트 카세트를 운반하는 플라스미드 pMMP184가 제조된 후에, BALB/c 마우스의 구강 경로를 경유하여 투여되었다. 총 IgG의 함량 변화는 χ8554 고스트 세포의 부스팅으로 발현 함량이 낮게 나타났지만, 3차 접종의 2주 경과 후, 6주차에서 증가되는 양상을 보였다. 그러나, 혼합 백신 그룹인 JOL389/χ8554 그룹에서는 총 IgG의 함량이 일차 접종 후 2주차부터 상승되는 경향을 보였고, 추가접종이 진행되므로써 많은 상승 폭을 나타내었다. 총 IgG의 함량은 백신 접종 후 10주차에서 χ8554그룹에 비교하여 JOL389/χ8554은 8배 이상 높은 것으로 관찰되었다. IgG1, IgG2a, 분비IgA의 함량은 백신화 후 4주차에서 상승되었다. 독력 살모넬라 타이피무리움 χ3339로 도전실험결과, χ8554 [pMMP184]과 χ8554 [pMMP184]/JOL389은 대조구에 비교하여 50% 이상의 보호효과가 관찰되었다. 이들 결과는 χ8554 [pMMP184]/JOL389은 χ8554 [pMMP184]보다 더 높은 면역 응답을 유도하는 것이 가능한 것으로 추정된다.

Keywords

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