Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
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NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells

사람 치주인대세포에서 Lipopolysaccharide와 니코틴으로 유도된 iNOS와 COX-2 발현에 NFATc의 관여

  • Lee, Sang-Im (Department of Dental Hygiene, School of Health Sciences, Dankook University) ;
  • Yu, Ji-Su (Department of Dental Hygiene, Gumi College)
  • 이상임 (단국대학교 보건과학대학 치위생학과) ;
  • 유지수 (구미대학교 치위생과)
  • Received : 2015.11.02
  • Accepted : 2015.11.11
  • Published : 2015.12.31
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Abstract

Although nuclear factor of activated T cell (NFAT) plays a key role in inflammation, its anti-inflammatory effects and mechanism of action in periodontitis are still unknown. This study aimed to identify the effects of NFAT on the proinflammatory mediators activated by lipopolysaccharide (LPS) plus nicotine stimulation in human periodontal ligament cells (hPDLCs). The production of nitric oxide (NO) and prostaglandin $E_2(PGE_2)$ was evaluated using Griess reagent and an enzyme immunoassay, respectively. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and NFAT proteins was evaluated by Western blot analysis. LPS plus nicotine synergistically induced the production of NO and $PGE_2$ and increased the protein expression of iNOS, COX-2 and NFAT. Treatment with an NFAT inhibitor blocked the LPS plus nicotine-stimulated NO and $PGE_2$ release as well as the expression of iNOS and COX-2. Our data suggest that the LPS plus nicotine-induced inflammatory effects on hPDLCs may act through a novel mechanism involving the action of NFAT. Thus, NFAT may provide a potential therapeutic target for the treatment of periodontal disease associated with smoking and dental plaque.

숙주 면역 반응과 면역 체계는 치주 질환에 대한 개인의 감수성의 주요 원인이다. 세균 감염과 흡연은 치주 조직의 파괴의 원인과 진행에 관여하는 중요한 환경 위험 요인이다. 따라서, 본 연구는 사람 치주인대세포에서 LPS와 니코틴이 전염증성 사이토카인인 iNOS/COX-2의 발현과 NO/$PGE_2$ 생산에 미치는 영향을 알아보고 NFATc1가 어떤 기전으로 항염작용을 하는지 밝히고자 하였다. LPS와 니코틴을 처리한 사람 치주인대세포에서 iNOS/COX-2의 발현과 함께 NO/$PGE_2$ 생산은 증가되었다. NFATc1 inhibitor인 CsA는 LPS와 니코틴에 의해 유도되는 iNOS/COX-2의 발현과 함께 NO/$PGE_2$ 생산을 감소시켰다. 이러한 연구 결과로 볼 때, NFAT signaling pathway가 LPS와 니코틴에 의한 iNOS/COX-2의 발현을 조절하여 NO/$PGE_2$ 매개 염증에 대해 방어할 수 있다고 생각된다.

Keywords

References

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