Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Molecular Identification and Distribution Pattern of Fish Eggs Collected around Jejudo Island

제주 연안해역 어란의 분자동정과 분포양상

  • Han, Song-Hun (Jeju Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Maeng Jin (Fisheries Resources and Environment Division, National Institute of Fisheries Science) ;
  • Song, Choon Bok (College of Ocean Sciences, Jeju National University)
  • 한송헌 (국립수산과학원 제주수산연구소) ;
  • 김맹진 (국립수산과학원 서해수산연구소) ;
  • 송춘복 (제주대학교 해양과학대학)
  • Received : 2015.10.01
  • Accepted : 2015.12.26
  • Published : 2015.12.31
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Abstract

Monthly variation of species composition and abundance of fish eggs were examined to know the spawning time and location of the fishes inhabiting the coastal region of Jejudo Island. Samplings had been performed at the four locations (Jeju port, Seongsanpo, Seogwipo port and Chagwido) with a bongo net which was towed monthly at the sea surface from August 2006 to July 2007. The fish eggs were identified based on phylogenetic analyses with the DNA sequences generated through PCR-amplification and sequencing of the mitochondrial cytochrome b gene. Among a total of 43 taxa classified during the study period, 34 were identified to species, 4 to families, and the remaining 5 were unidentified. Of them, 23 taxa were occurred at Jeju port, 21 at Seongsanpo, 19 at Seogwipo port and 18 at Chagwido, whereas 15 taxa were found in September 2006, 12 in June 2007, 6 to 8 in August 2006 and January~May 2007, 5 in each October and November 2006, 3 in each December 2006 and July 2007. Among 34 species of fish eggs, Engraulis japonicus and Callanthias japonicus most frequently appeared at 16 times out of 48 observations in total. When those eggs were collected, the surface seawater temperature ranged $14.0{\sim}28.6^{\circ}C$ for E. japonicus and $14.9{\sim}20.5^{\circ}C$ for C. japonicus. The success rates of PCR-amplification and species identification in this study were 68.3% and 79.1%, respectively.

이 연구는 제주도 주변 4개의 정점에서 채집된 어란의 월별 종조성 및 출현량을 알아보기 위해 실시하였다. 현장조사는 2006년 8월부터 2007년 7월까지 봉고네트를 이용하여 월별로 표층채집을 하였으며, 종동정은 채집된 어란의 미토콘드리아 cytochrome b 유전자 (cyt b) 염기서열을 성어 염기서열과 상호 비교함으로써 이루어졌다. 채집된 어란은 총 43개의 분류군으로 분류되었고, 이중 34종 (6목 24과 33속)은 종 (species)까지 동정이 이루어졌다. 나머지 9종 가운데 4종은 과 (family)까지 동정할 수 있었고, 5종은 미동정되었다. 지역별로 제주항 근해에서는 23개의 분류군으로 가장 많은 분류군이 나타났고, 성상포 근해에서 21개, 서귀포항 근해에서 19개, 차귀도 근해에서는 18개의 분류군이 출현하였다. 시기별로는 2006년 9월에 15개의 분류군으로 가장 많이 나타났고, 12월과 7월에 3개의 분류군으로 가장 적게 나타났다. 동정이 이루어진 34종 중 멸치 (E. japonicus)와 노랑벤자리 (C. japonicus)가 가장 높은 출현 빈도를 보였으며, 멸치는 표층수온 $14.0{\sim}28.6^{\circ}C$에서, 노랑벤자리는 $14.9{\sim}20.5^{\circ}C$에서 출현하였다. 이번 연구에서 사용된 cyt b 유전자의 PCR 증폭 성공률은 68.3%였으며, 종동정률은 79.1%였다.

Keywords

References

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