한국산 미꾸리과 어류 왕종개 Iksookimia longicorpa 망막의 시각세포

Visual Cells in the Retina of Iksookimia longicorpa (Pisces; Cobitidae) of Korea

  • 김재구 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생물학과, 전북대학교 생물다양성연구소)
  • Kim, Jae Goo (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Park, Jong Young (Faculty of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 투고 : 2015.12.09
  • 심사 : 2015.12.21
  • 발행 : 2015.12.31

초록

한국고유종인 왕종개의 안구를 관찰하고 망막에 존재하는 시각세포의 구조를 광학현미경과 주사전자현미경을 이용하여 조사하였다. 왕종개의 안구는 $3.53{\pm}0.2mm$의 크기였으며 두장대 안경비율은 $14.08{\pm}0.7$이였다. 시각세포의 수직단면을 확인한 결과 망막은 여러 층으로 구성되어 있었으며 전체 망막의 두께는 $216.42({\pm}13.36){\mu}m$였으며, 시각세포의 핵이 존재하는 바깥핵층의 두께는 13.42 $({\pm}2.16){\mu}m$였다. 이형 이중 원추세포 중 긴 원추세포는 $26.42{\pm}1.7{\mu}m$의 길이였으며 직경은 $5.12{\pm}0.6{\mu}m$로 확인되었다. 짧은 원추세포는 $16.82{\pm}1.1{\mu}m$의 길이였으며 직경은 긴 원추세포와 유사한 $5.10{\pm}0.31{\mu}m$로 확인되었다. 간상세포는 길이 $40.13{\pm}2.4{\mu}m$였으며 직경 $3.95{\pm}0.4{\mu}m$로 확인되었다. 시각세포의 수평단면을 확인한 결과 이형 이중 원추세포가 규칙적인 배열로 인해 열 배열(row-type)의 모자이크 패턴으로 나타났다. 긴 원추세포와 짧은 원추세포가 하나의 쌍을 이루었으며 긴 원추세포는 내절이 절단되어 eosin에 염색되었으며 짧은 원추세포는 외절이 절단되어 hematoxylin에 염색되었다. 전자현미경을 통해 미세구조를 확인한 결과 내절과 외절로 구분되고 내절은 장타원형으로 확인되었다. 외절에는 디스크구조의 판상을 확인할 수 있었으며 내절과 외절을 연결해주는 배상극 (calyceal processes)들이 가장자리에 분포하고 있었다.

The visual cells in the retina of Iksookimia longicorpa (Pisces, Cobitidae) were investigated by light and scanning electron microscopes. The retina ($216.42{\pm}13.36{\mu}m$) has several layers, and the visual cell layer consists of unequal double cones and large rods. In a double cone, two members are unequal such that one cone is longer than the other (long element $26.42{\pm}1.7{\mu}m$, short element $16.82{\pm}1.1{\mu}m$). The cones form a row mosaic pattern in which the partners of double cones are linearly oriented with a large rod. The visual cells observed have an outer segment (hematophilic), inner segment (eosinophilic). In scanning electron microscopy, the outer segment links to inner segment by so-called calyceal piles (calyceal processes) of membrane discs surrounded by double membranes.

키워드

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