Biomaterials and Biomechanics in Bioengineering

Techno-Press (테크노프레스)
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Fabrication of multi-well platform with electrical stimulation for efficient myogenic commitment of C2C12 cells

  • Song, Joohyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Eunjee A. (School of Chemical and Biological Engineering, Seoul National University) ;
  • Cha, Seungwoo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Insun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Choi, Yonghoon (School of Chemical and Biological Engineering, Seoul National University) ;
  • Hwang, Nathaniel S. (School of Chemical and Biological Engineering, Seoul National University)
  • Received : 2015.02.12
  • Accepted : 2015.02.25
  • Published : 2015.03.25
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Abstract

To engineer tissue-like structures, cells are required to organize themselves into three-dimensional networks that mimic the native tissue micro-architecture. Here, we present agarose-based multi-well platform incorporated with electrical stimulation to build skeletal muscle-like tissues in a facile and highly reproducible fashion. Electrical stimulation of C2C12 cells encapsulated in collagen/matrigel hydrogels facilitated the formation 3D muscle tissues. Consequently, we confirmed the transcriptional upregulations of myogenic related genes in the electrical stimulation group compared to non-stimulated control group in our multi-well 3D culture platform. Given the robust fabrication, engineered muscle tissues in multi-well platform may find their use in high-throughput biological studies drug screenings.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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