Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)

  • Kwon, Hyuk Woo (Department of Microbiology and Institute of Biodiversity, Dankook University) ;
  • Choi, Min Ah (Department of Microbiology and Institute of Biodiversity, Dankook University) ;
  • Yun, Yeo Hong (Department of Microbiology and Institute of Biodiversity, Dankook University) ;
  • Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Seong Hwan (Department of Microbiology and Institute of Biodiversity, Dankook University)
  • Received : 2015.02.28
  • Accepted : 2015.03.03
  • Published : 2015.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of IGS I, which ranged from 1,301 to 1,348 bp, were present among twenty ASI1346-derived progeny strains, while 15 different sizes of IGS I, which ranged from 700 to 1,347 bp, were present among twenty ASI1038-derived progeny strains. Phylogenetic analysis of the IGS sequences revealed that different clades were present in both the ASI10388- and ASI1346-derived progeny strains. Plating assays of seven kinds of extracellular enzymes (${\beta}$-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease) also revealed apparent variation in the ability to produce extracellular enzymes among the 40 tested progeny strains from both parental A. bisporus strains. Overall, this study demonstrates that characterization of IGS I regions and extracellular enzymes is useful for the assessment of the substrate-degrading ability and heterogenicity of A. bisporus monokaryotic strains.

Keywords

References

  1. Fritsche G. Breeding mushrooms. Mushroom J 1986;157:4-17.
  2. Raper CA, Raper JR, Miller RE. Genetic analysis of the life cycle of Agaricus bisporus. Mycologia 1972;64:1088-117. https://doi.org/10.2307/3758075
  3. Callac P, Imbernon M, Kerrigan RW, Olivier JM. The two life cycles of Agaricus bisporus. In: Royse DJ, editor. Mushroom Biology and Mushroom Products: Proceedings of the 2nd International Conference; 1996 Jun 9-12; University Park, PA, USA. University Park: The Pennsylvania State University;1996. p. 57-66.
  4. Kerrigan RW, Royer JC, Baller LM, Kohli Y, Horgen PA, Anderson JB. Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus. Genetics 1993;133:225-36.
  5. Min KJ, Kim JK, Kwak AM, Kong WS, Oh YH, Kang HW. Genetic diversity of Agaricus bisporus strains by PCR polymorphism. Kor J Mycol 2014;42:1-8. https://doi.org/10.4489/KJM.2014.42.1.1
  6. Kim SH, Uzunovic A, Breuil C. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl Environ Microbiol 1999;65:287-90.
  7. Vilgalys R, Gonzalez D. Organization of ribosomal DNA in the basidiomycete Thanatephorus praticola. Curr Genet 1990;18:277-80. https://doi.org/10.1007/BF00318394
  8. Vilgalys R, Hopple JS Jr, Hibbett DS. Phylogenetic implications of generic concepts in fungal taxonomy: the impact of molecular systematic studies. Mycol Helv 1994;6:73-91.
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  10. Morton A, Tabrett AM, Carder JH, Barbara DJ. Sub-repeat sequences in the ribosomal RNA intergenic regions of Verticillium alboatrum and V. dahliae. Mycol Res 1995;99:257-66. https://doi.org/10.1016/S0953-7562(09)80895-2
  11. Ganley AR, Scott B. Extraordinary ribosomal spacer length heterogeneity in a Neotyphodium endophyte hybrid: implications for concerted evolution. Genetics 1998;150:1625-37.
  12. Li L, Zhong CH, Bian YB. The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer. Electron J Biotechnol 2014;17:27-33. https://doi.org/10.1016/j.ejbt.2013.12.005
  13. Selosse MA, Costa G, Di Battista C, Le Tacon F, Martin F. Meiotic segregation and recombination of the intergenic spacer of the ribosomal DNA in the ectomycorrhizal basidiomycete Laccaria bicolor. Curr Genet 1996;30:332-7. https://doi.org/10.1007/s002940050141
  14. Saito T, Tanaka N, Shinozawa T. Characterization of subrepeat regions within rDNA intergenic spacers of the edible basidiomycete Lentinula edodes. Biosci Biotechnol Biochem 2002;66:2125-33. https://doi.org/10.1271/bbb.66.2125
  15. Kwon HW, Kim JY, Min SH, Choi MA, Oh YL, Kong WS, Kim SH. Biochemical characterization of Agaricus bisporus dikaryon strains. Kor J Mycol 2014;42:86-90. https://doi.org/10.4489/KJM.2014.42.1.86
  16. Kwon HW, Back IJ, Ko HG, You CH, Kim SH. Extracellular enzyme activities of the monokaryotic strains generated from basidiospores of shiitake mushroom. Mycobiology 2008;36:74-6. https://doi.org/10.4489/MYCO.2008.36.1.074