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Optimization of Extraction Conditions of Sarcodon aspratus by Response Surface Methodology

반응표면분석에 의한 능이버섯 추출물의 추출조건 최적화

  • Received : 2014.12.01
  • Accepted : 2015.02.25
  • Published : 2015.03.31

Abstract

Response surface methodology (RSM) was used to monitor extraction characteristics of extracts from Sarcodon aspratus. Based on a central composite design, independent variables were microwave power (30~150 W), ethanol concentration (0~100%), and extraction time (1~9 min). Dependent variables were yield, electron-donating ability, total phenol contents, and SOD-like activity. Coefficients of determination ($R^2$) for dependent variables ranged from 0.80 at 0.97. The maximum extraction yield was 50.28% under conditions of 125.1 W microwave power, 18.67% ethanolic concentration, and 7.06 min extraction time. The maximum extraction electron-donating ability was 22.14% under conditions of 31.09 W, 45.76%, and 4.32 min. The maximum extraction total polyphenol content was 30.54 mg tannic acid equivalent/g at 122.54 W, 48.05%, and 8.36 min. The maximum extraction SOD-like activity was 33.44% at 121.17 W, 47.42%, and 8.41 min. Based on superimposition of four dimensional RSM with respect to extraction yield, electron-donating ability, total polyphenol content, and SOD-like activity obtained under various extraction conditions, optimum ranges of extraction conditions were found to be microwave power of 78~88 W, ethanol concentration of 39~57%, and extraction time of 3.5~9 min.

능이버섯의 수율과 항산화 활성을 측정하여 효율적 추출조건을 예측하기 위해 마이크로웨이브 에너지, 에탄올 농도, 추출시간의 요인 변수를 중심합성법에 따라 설정하여 반응 표면분석을 통해 최적화 조건을 설정하였다. 능이버섯 추출물의 수율, 전자공여작용, 총 폴리페놀 함량, SOD 유사 활성에 대한 반응표면의 회귀식 $R^2$값이 각각 0.97, 0.90, 0.80 및 0.87로, 수율, 전자공여작용 및 SOD 유사 활성이 각각 P<0.001, P<0.05 및 P<0.05 수준에서 유의성이 인정되었다. 능이버섯은 요인 변수에 따라 수율은 마이크로웨이브 에너지의 영향을 가장 많이 받았고, 에탄올 농도에 따른 영향도 있었다. 그 외 종속변수인 전자공여능과 SOD 유사 활성은 에탄올 농도에 따른 영향을 받는 것으로 나타났다. 추출물의 각 특성을 모두 만족시키는 최적의 추출조건의 범위는 superimposing 하여 마이크로웨이브 에너지 78~88 W, 에탄올 농도 39~57%, 추출시간 3.5~9분으로 예측되었다.

Keywords

References

  1. Kim GJ, Kim HS, Chung SY. 1992. Effects of varied mushroomon lipid compositions in dietary hypercholesterolemic rats. J Korean Soc Food Nutr 21: 131-135.
  2. Astrup T, Stermdoff I. 1956. The plasminogen activator in animal tissue. Acta Physiol Scand 36: 250-255. https://doi.org/10.1111/j.1748-1716.1956.tb01322.x
  3. Yoo JE, Lee YN. 2009. Optimal culture conditions for MK1 strain isolated from soft-rotten tissue of Neungee mushroom (Sarcodon aspratus) and the physico-chemical properties of the purified exopolysaccharide of MK1. Korean J Microbiol 45: 324-331.
  4. Kang TS, Kang MS, Sung JM, Kang AS, Shon HR, Lee SY. 2001. Effect of Pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Korean J Mycol 29: 86-90.
  5. Jeong OJ, Yoon HS, Min YK. 2001. Aroma characteristics of Neungee (Sarcodon aspratus). Korean J Food Sci Technol 33: 307-312.
  6. Song JH, Lee HS, Hwang JK, Han JW, Ro JG, Keum DH, Park KM. 2003. Physiological activity of Sarcodon aspratus extracts. Korean J Food Sci Ani Resour 23: 172-179.
  7. Lee SA, Song YS, Cho JW, Lee JH, Cho JS. 2001. Effect of the Sarcodon aspratus on the physicochemical and sensory properties of cooked beef. J Korean Soc Food Sci Nutr 30: 266-272.
  8. Lee JH, Yang JL, Jung CS, Kim HS, Cho JS. 2001. Isolation and purification of fibrinolytic enzyme of edible mushroom, Sarcodon aspratus (Berk.) S. Ito. Korean J Life Sci 11: 561-567.
  9. Nilsson-Ehle P, Garfinkel AS, Schotz MC. 1980. Lipolytic enzymes and plasma lipoprotein metabolism. Annu Rev Biochem 49: 667-693. https://doi.org/10.1146/annurev.bi.49.070180.003315
  10. Yoon KY, Lee SH, Shin SR. 2006. Antioxidant and antimicrobial activities of extracts from Sarcodon aspratus. J Korean Soc Food Sci Nutr 35: 967-972. https://doi.org/10.3746/jkfn.2006.35.8.967
  11. Kim JW, Moon BS, Park YM, Yoo NH, Ryoo IJ, Chinh NT, Yoo ID, Kim JP. 2005. Structures and antioxidant activity of diketopiperazines isolated from the mushroom Sarcodon aspratus. J Korean Soc Appl Biol Chem 48: 93-97.
  12. Uhm TB, Ryu KS, Kim MK, Yoo JS, Sohn HS, Lee TK. 1991. Characterization of a serine protease from Neungee [Sarcodon aspratus (Berk.) S. Ito]. J Korean Soc Food Nutr 20: 35-39.
  13. Zhang M, Cui SW, Cheung PC, Wang Q. 2007. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 18: 4-19. https://doi.org/10.1016/j.tifs.2006.07.013
  14. Kang HG, Zanabaatar B, Lee JS, Seo GS, Lee JS. 2011. Antihypertensive activity and anti-gout activity of mushroom Sarcodon aspratus. Korean J Mycol 39: 53-56. https://doi.org/10.4489/KJM.2011.39.1.053
  15. Lee KS, Kim JB. 2009. Effects of the Sarcodon aspratus on the high level of blood lipid and obesity induce by high fat-diet in rat. J Life Sci 19: 1265-1270. https://doi.org/10.5352/JLS.2009.19.9.1265
  16. Mizuno M, Shiomi Y, Minato K, Kawakami S, Ashida H, Tsuchida H. 2000. Fucogalactan isolated from Sarcodon aspratus elicits release of tumor necrosis factor-alpha and nitric oxide from murine macrophages. Immunopharmacology 46: 113-121. https://doi.org/10.1016/S0162-3109(99)00163-0
  17. Kwon JH, Belanger JM, Pare JR. 1992. Optimization of microwave-assisted extraction (MAP) for ginseng components by response surface methodology. J Agric Food Chem 51: 1807-1810.
  18. Kwon JH, Belanger JMR, Pare JRJ, Yaylayan VA. 2003. Application of microwave-assisted process (MAP) to the fast extraction of ginseng saponins. Food Res Int 36: 491-498. https://doi.org/10.1016/S0963-9969(02)00197-7
  19. Park EJ, An JJ, Kim JS, Kwon JH. 2013. Antioxidant activities in freeze-dried and hot air-dried Schizandra fruit (Schizandra chinensis Baillon) at different microwave-assisted extraction conditions. Korean J Food Sci Technol 45: 667-674. https://doi.org/10.9721/KJFST.2013.45.6.667
  20. Turkmen N, Sari F, Sedat Velioglu Y. 2006. Effect of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem 99: 835-841. https://doi.org/10.1016/j.foodchem.2005.08.034
  21. Jeong JE, Shim SP, Jeong YS, Jung HK, Kim YC, Hong JH. 2011. Optimization of extraction conditions for ethanol extracts from Citrus unshiu peel by response surface methodology. Korean J Food Preserv 18: 755-763. https://doi.org/10.11002/kjfp.2011.18.5.755
  22. Kim DY, Teng H, Choi YH. 2012. Optimization of ultrasonic-assisted extraction process for Inonotus obliquus using response surface methodology. Current Research on Agriculture and Life Sciences 30: 68-75.
  23. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1120. https://doi.org/10.1038/1811199a0
  24. Folin O, Denis W. 1912. On phosphotungastic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-249.
  25. Marklund S, Marklund G. 1975. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.
  26. Kang BH, Lee JM, Kim YK. 2010. Optimization of hot water extraction conditions for Tricholoma matsukake by response surface methodology. J Korean Soc Food Sci Nutr 39: 1206-1212. https://doi.org/10.3746/jkfn.2010.39.8.1206
  27. Lee JH, Kim Y, Lee SY, Yoo SH. 2014. Conditions for obtaining optimum polyphenol contents and antioxidant activities of Korean berry and green tea extracts. Korean J Food Sci Technol 46: 410-417. https://doi.org/10.9721/KJFST.2014.46.4.410
  28. Choi MA, Park NY, Woo SM, Jeong YJ. 2003. Optimization of extraction conditions from Hericium erinaceus by response surface methodology. Korean J Food Sci Technol 35: 777-782.

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