Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Oral Concentrated Grape Juice Suppresses Expression of NF-kappa B, TNF-α and iNOS in Experimentally Induced Colorectal Carcinogenesis in Wistar Rats

  • Published : 2015.03.04
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to evaluate the effects of grape juice on colon carcinogenesis induced by azoxymethane (AOM) and expression of NF-kB, iNOS and TNF-${\alpha}$. Methods: Forty male Wistar rats were divided into 7 groups: G1, control; G2, 15 mg/kg AOM; G3, 1% grape juice 2 weeks before AOM; G4, 2% grape juice 2 weeks before AOM; G5, 1% grape juice 4 weeks after AOM; G6, 2% grape juice 4 weeks after AOM; G7, 2% grape juice without AOM. Histological changes and aberrant crypt foci (ACF) were studied, while RNA expression of NF-kB, TNF- and iNOS was evaluated by qPCR. Results: The number of ACF was higher in G2, and G4 presented a smaller number of crypts per focus than G5 (p=0.009) and G6. Small ACF (1-3) were more frequent in G4 compared to G2, G5 and G6 (p=0.009, p=0.009 and p=0.041, respectively). RNA expression of NF-kB was lower in G3 and G4 compared to G2 (p=0.004 and p=0.002, respectively). A positive correlation was observed between TNF-${\alpha}$ and NF-kB gene expression (p=0.002). In conclusion, the administration of 2% grape juice before AOM reduced the crypt multiplicity, attenuating carcinogenesis. Lower expression of NF-kB was observed in animals exposed to grape juice for a longer period of time, regardless of concentration.

Keywords

References

  1. Aguiar O Jr, Gollucke AP, de Moraes BB, et al (2011). Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet. Br J Nutr, 105, 694-702. https://doi.org/10.1017/S0007114510004368
  2. Aggarwal BB, Bhardwaj A, Aggarwal RS, et al (2004). Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res, 24, 2783-840.
  3. Alrawi SJ, Schiff M, Carroll RE, et al (2006). Aberrant crypt foci. Anticancer Res, 26, 107-19.
  4. Arulselvan P, Wen CC, Lan CW, et al (2012). Dietary administration of scallion extract effectively inhibits colorectal tumor growth: cellular and molecular mechanisms in mice. PLoS One, 7, 44658. https://doi.org/10.1371/journal.pone.0044658
  5. Aviello G, Romano B, Borrelli F, et al (2012). Chemopreventive effect of the non-psychotropic phytocannabinoid cannabidiol on experimental colon cancer. J Mol Med, 90, 925-34. https://doi.org/10.1007/s00109-011-0856-x
  6. Azzolina A, Bongiovanni A, Lampiasi N (2003). Substance P induces TNF-alpha and IL-6 production through NF kappa B in peritoneal mast cells. Biochim Biophys Acta, 1643, 75-83. https://doi.org/10.1016/j.bbamcr.2003.09.003
  7. Baldwin AS (2001). Control of carcinogenesis and cancer therapy resistance by the transcription factor NF-Kappa B. J Clin Invest, 107, 241-246. https://doi.org/10.1172/JCI11991
  8. Bird RP (1995). Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett, 93, 55-71. https://doi.org/10.1016/0304-3835(95)03788-X
  9. Block TM, Mehta AS, Fimmel CJ, Jordan R (2003) Molecular viral oncology of hepatocellular carcinoma. Oncogene, 22, 5093-107. https://doi.org/10.1038/sj.onc.1206557
  10. Cilloni D, Messa F, Rosso V, et al (2008). Increase sensitivity to chemotherapeutical agents and cytoplasmatic interaction between NPM leukemic mutant and NF-kappaB in AML carrying NPM1 mutations. Leukemia, 22, 1234-40. https://doi.org/10.1038/leu.2008.68
  11. Femia AM, Caderni G (2008). Rodent models of colon carcinogenesis for the study of chemopreventive activity of natural products. Planta Med, 74, 1602-07. https://doi.org/10.1055/s-2008-1074577
  12. Hamilton W, Lancashire R, Sharp D, et al (2009). The risk of colorectal cancer with symptoms at different ages and between the sexes: a case-control study. BMC Med, 7, 17. https://doi.org/10.1186/1741-7015-7-17
  13. Hasler CM, Bloch AS, Thomson CA, Enrione E, Manning C (2004). Position of the American Dietetic Association:functional foods. J Am Diet Assoc, 5, 814-26.
  14. Jemal A, Siegel R, Xu J, et al (2010). Cancer statistics. Cancer J Clin, 60, 277-300. https://doi.org/10.3322/caac.20073
  15. Li TW, Yang H, Peng H, et al (2012). Effects of S-adenosylmethionine and methylthioadenosine on inflammation-induced colon cancer in mice. Carcinogenesis, 33, 427-35. https://doi.org/10.1093/carcin/bgr295
  16. Lind DS, Hochwald SN, Malaty J, et al (2001). NF-kB is upregulated in colorectal cancer. Surgery, 130, 363-9. https://doi.org/10.1067/msy.2001.116672
  17. Marshall JR (2008). Prevention of colorectal cancer: diet, chemoprevention and lifestyle. Gastroenterol Clin North Am, 37, 73-81 https://doi.org/10.1016/j.gtc.2007.12.008
  18. Manson MM (2003). Cancer prevention-the potential for diet to modulate molecular signalling. Trends Mol Med, 9, 11-8. https://doi.org/10.1016/S1471-4914(02)00002-3
  19. Meteoglu I, Erdogdu IH, Meydan N, Erkus M, Barutca S (2008). NF-KappaB expression correlates with apoptosis and angiogenesis in clear cell renal cell carcinoma tissues. J Exp Clin Cancer Res, 27, 53. https://doi.org/10.1186/1756-9966-27-53
  20. Moreira L (2012). Update on colorectal cancer precursor lesions. Gastroenterol Hepatol, 1, 80-5.
  21. Morris GP, Beck PL, Herridge MS, et al (1989). Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology, 96, 795-803.
  22. Murakami A (2009). Chemoprevention with phytochemicals targeting inducible nitric oxide synthase. Forum Nutr, 61, 193-203. https://doi.org/10.1159/000212751
  23. Olivier S, Robe P, Bours V (2006). Can NF-kappaB be a target for novel and efficient anti-cancer agents? Biochem Pharmacol, 72, 1054-68. https://doi.org/10.1016/j.bcp.2006.07.023
  24. Perse M, Cerar A (2011). Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon. J Biomed Biotechnol, 2011, 473964
  25. Pikarsky E, Porat RM, Stein I, et al (2004). NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature, 431, 461-6. https://doi.org/10.1038/nature02924
  26. Popivanova BK, Kitamura K, Wu Y, et al (2008). Blocking TNFalpha in mice reduces colorectal carcinogenesis associated with chronic colitis. J Clin Invest, 118, 560-70.
  27. Surh YJ. 2003. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer, 3, 768-80 https://doi.org/10.1038/nrc1189
  28. Terzic J, Grivennikov S, Karin E, Karin M (2014). Inflammation and colon cancer. Gastroenterology, 138, 2101-14.
  29. Umesalma S, Sudhandiran G (2010). Differential inhibitory effects of the polyphenol ellagic acid on inflammatory mediators NF-kappaB, iNOS, COX-2, TNF-alpha, and IL-6 in 1,2-dimethylhydrazine-induced rat colon carcinogenesis. Basic Basic Clin Pharmacol Toxicol, 107, 650-5. https://doi.org/10.1111/j.1742-7843.2010.00565.x
  30. Velmurugan B, Singh RP, Agarwal R, Agarwal C (2010). Dietaryfeeding of grape seed extract prevents azoxymethaneinduced colonic aberrant crypt foci formation in fischer rats. Mol Carcinog, 49, 641-52.
  31. Wallerath T, Deckert G, Ternes T, et al (2002). Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase. Circulation, 106, 1652-8. https://doi.org/10.1161/01.CIR.0000029925.18593.5C
  32. Watanabe K, Kawamori T, Nakatsugi S, Wakabayashi K. (2000) COX-2 and iNOS, good targets for chemoprevention of colon cancer. Biofactors, 12, 129-33. https://doi.org/10.1002/biof.5520120120
  33. Yasui Y, Miyamoto S, Kim M, et al (2008). Aqueous and ethanolic extract fractions from the Brazilian propolis suppress azoxymethane-induced aberrant crypt foci in rats. Oncol Rep, 20, 493-9.

Cited by

  1. Unpolished Thai Rice Prevents ACF Formation and Dysplastic Progression in AOM-Induced Rats and Induces Apoptosis Through Redox Alteration in CaCo-2 Cells vol.16, pp.7, 2015, https://doi.org/10.7314/APJCP.2015.16.7.2827
  2. Vitamin D3 enhances the tumouricidal effects of 5-Fluorouracil through multipathway mechanisms in azoxymethane rat model of colon cancer vol.34, pp.1, 2015, https://doi.org/10.1186/s13046-015-0187-9
  3. B-Dependent Autophagy vol.2017, pp.1942-0994, 2017, https://doi.org/10.1155/2017/7085709
  4. Crocin synergistically enhances the antiproliferative activity of 5-flurouracil through Wnt/PI3K pathway in a mouse model of colitis-associated colorectal cancer vol.119, pp.12, 2018, https://doi.org/10.1002/jcb.27367
  5. Protective effect of grape or apple juices in bone tissue of rats exposed to cadmium: role of RUNX-2 and RANK/L expression vol.25, pp.16, 2018, https://doi.org/10.1007/s11356-018-1778-8