미생물학회지 (Korean Journal of Microbiology)

  • 제51권4호
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  • Pages.382-388
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  • 2015
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
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제주 조간대로부터 단백질 가수분해효소를 생산하는 세균의 분리 및 동정

Isolation and identification of protease-producing bacteria from the intertidal zone in Jeju Island, Korea

  • 문영건 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 수브라마니안 다라니다란 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 김동휘 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 박소현 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 허문수 (제주대학교 해양생명과학대학 수산의생명과학부)
  • Moon, Young-Gun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Dharaneedharan, Subramanian (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Kim, Dong-Hwi (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Park, So-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Heo, Moon Soo (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University)
  • 투고 : 2015.11.04
  • 심사 : 2015.12.24
  • 발행 : 2015.12.31
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초록

단백질가수분해효소를 생산하는 11개의 세균들은 유기 생물체의 외부 표면 서식하며, inorganic materials는 제주도 조간대로부터 수집되었다. 시료들은 냉동 상태로 실험실로 옮겨졌으며, 멸균 해수와 1% skim milk가 들어가 있는 Zobell plates에서 배양시켰다. 다음 clear zone이 나타난 11개의 균주들은 단백질분해효소를 생성하는 세균으로서 선택되었으며, 각각의 균주들은 16S rDNA을 기반으로 동정하였다. 분석결과, Psedoalteromonas속 해양 세균 JJM125, JJM129, YG47과 YG49, Microbulbifer속 JJM122, Vibrio속 YG51, YG52, YG62, YG63, Firmicutes문과 Bacillaceae강에 속하는 JJM129, YG65로 나타났다. 따라서, 본 연구에서는 단백질분해가수효소를 생성하는 세균들을 다양한 생명 공학 응용프로그램과 함께 새로운 다양성의 개발 및 이용이 가능할 것이다.

Eleven protease-producing bacteria were isolated from the organisms' external shells and the inorganic materials collected from intertidal zone of Jeju Island, Republic of Korea. The samples were diluted serially, inoculated on Zobell agar plates with 1% skim milk and incubated at $20^{\circ}C$. Clear zone forming colonies were selected as protease-producing bacteria and each strain was identified based on the phylogenetic analysis with their 16S rDNA sequences. Strains JJM125, JJM129, YG47 and YG49 belong to the marine bacterial genus Pseudoalteromonas; strain JJM122 belong to the genus Microbulbifer; strains YG51, YG52, YG62 and YG63 belong to the genus Vibrio; and strain YG65 belong to genus Bacillus. Hence, the present study suggests that these protease producing bacteria could be further used to develop new varieties of protease with various biotechnological applications.

키워드

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