Egg Development and Morphology of Larvae and Juveniles of Spotted Knifejaw, Oplegnathus punctatus

강담돔 (Oplegnathus punctatus)의 난 발생 및 자치어 형태발달

  • 박재민 (경상북도 토속어류산업화센터) ;
  • 이성훈 (전남대학교 양식생물학전공) ;
  • 윤성민 (경상북도 민물고기연구센터) ;
  • 나해춘 (세보수산) ;
  • 한경호 (전남대학교 양식생물학전공)
  • Received : 2015.02.09
  • Accepted : 2015.04.24
  • Published : 2015.06.30

Abstract

Morphological changes in eggs and larvae of spotted knifejaw, Oplegnathus punctatus were observed by cultivation of fertilized eggs to juvenile stage. Fertilized egg was demersal and transparent. The egg has one oil glouble and its size ranged 1.01~1.15 mm ($1.08{\pm}0.10mm$; $mean{\pm}SD$, n=20). Culture chambers were maintained $22.5{\sim}24.5^{\circ}C$ in water temperature and 33.5~34.5ppt in salinity. It took 25 mins from fertilization to the blastoderm stage, 55 mins to 2 cell stage and 20 hrs 50 mins to hatching. Newly hatched yolksac larvae ranged 2.59~3.02 mm ($2.81{\pm}0.25mm$, n=5) in total length, and mouth and anus were not still open. Yolk and oil globule were absorbed 2 days after hatching (DAH). Flexion larvae in 12 DAH ranged 3.02~3.07 mm TL ($3.04{\pm}0.04mm$), and the caudal fin grew to fan-shaped, melanophores appeared on top of digestive canal.

강담돔의 수정난을 부화시켜 사육하면서 난 발생 과정과 자치어 발달과정의 형태변화를 관찰하였다. 수정란은 구형의 무색 투명한 분리 부성란이었고, 1개의 유구를 가지고 있었다. 난의 크기는 1.01~1.15 mm 범위였다 (평균 $1.08{\pm}0.10mm$, n=20). 사육수온은 $22.5{\sim}24.5^{\circ}C$ (평균 $23.0{\pm}0.05^{\circ}C$), 염분농도는 33.5~34.5‰ (평균 $34.0{\pm}0.05$‰)의 범위를 유지시켜 주었다. 난 발생 과정은 수정 25분 후에 난막과 난황이 분리되면서 배반이 형성되었다. 수정 후 55분에는 난할이 2개로 분리되어 2세포기에 달하였다. 수정 후 20시간 50분에는 배체가 머리 부분부터 난막을 뚫고 나오면서 부화하였다. 부화 직후의 난황자어는 전장 2.10~2.20 mm (평균 $2.15{\pm}0.07mm$, n=10)로 입과 항문은 열려 있지 않았고, 난황과 유구는 부화 2일 후 흡수되었다. 부화 후 12일째 중기자어는 전장 4.43~4.80 mm(평균 $4.62{\pm}0.26mm$)로 꼬리지느러미는 부채꼴 모양으로 발달하였고, 흑색소포는 소화관 위쪽으로 침착하였다.

Keywords

References

  1. Blaxter, J.H.S. 1974. The early life history of fish. Spring-Verlag Berlin, Heidelberg, 765pp.
  2. Byun, S.G., S.H. Lee, J.H. Hwang, K.H. Han, K.W. Kang, J.D. Kim, Y.C. Kim and B.I. Lee. 2009. Embryonic and Larva Development of Slime Flounder, Microstomus achne. Dev. Reprod., 13: 281-289. (in Korean)
  3. Go, J.L. and Y.U. Kim. 1992. Embryonic development and morphology of larvae and juveniles of parrot Fish, Oplegnathus fasciatus (Temminck et Schlegel). PhD thesis, Pukyong Nat. Univ., 32: 29-45. (in Korean)
  4. Han, H.K., D.Y. Kang, S.B. Hur and C.Y. Jeon. 2001. Effects of Salinity on Spontaneous Maturation and Induced Spawning in Seabass, Lateolabrax japonicus. Kor. J. Aquacult., 14: 213-220. (in Korean)
  5. Han, K.H. and Y.U. Kim. 1997. The early life history of the flounder, Paralichthys olivaceus I. Development of egg, larvae and juveniles. Bull. Yeosu Nat. Fish. Univ., 11: 105-117. (in Korean)
  6. Hwang, H.K. 2000. Parrot Fish, Oplegnathus fasciatus of aquaculture. Ministry of Oceans and Fisheries, pp. 13-16. (in Korean)
  7. Hwang, H.K., J.U. Lee, S.G. Yang, S.C. Kim and K.M. Kim. 2005. Water Temperature and Food on Growth and Survival of Parrot Fish Larvae, Oplegnathus fasciatus. Kor. J. Aquacult., 18: 13-18. (in Korean)
  8. Ishiguro, N. and M. Nishida. 1998. A tailless spotted parrot fish Oplegnathus fasciatus lacking the caudal fin. Japan. J. Ichthyol., 45: 43-45.
  9. Jeong, K.S., S.M. Kim, I.C. Bang, S.Y. Kim and W.K. Lee. 1998. Induced Spawning of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Water Temperature and Photoperiod. Kor. J. Aquacult., 11: 141-149. (in Korean)
  10. Kakizawa, Y., K. Kamishikiryo, M. Shirato, S. Maehara, H. Fuji and S. Iesato. 1980. The tooth development of the Parrot Perch, Oplegnathus fasciatus, (family Oplegnathidae, Teleostei). J. Nihon Univ. School of Dentistry, 4: 211-226.
  11. Kawabe, K. and J. Kimura. 2007. Broodstock rearing and egg-taking from spotted parrot fish Oplegnathus punctatus in Chichijima, Ogasawara Islands, Southern Japan. Aqua. Sci., 55: 135-136.
  12. Kendall, A.W. Jr., E.H. Ahlstrom and H.G. Moser. 1984. Early life history stages of fishes and their characters. In: Moser, H.G. et al. (eds.), Ontogeny and Systematics of Fishes. Am. Soc. Ichthyol. Herpetol., Spec. Publ., 1: 11-22, Allen Press, Lawrence, KS.
  13. Kim, B.H. 1998. Sex differentiation and Gonad development of the Parrot fish, Oplegnathus fasciatus. Jeju Univ., pp. 1-32. (in Korean)
  14. Kim, G.J. 2007. Studies on the production of seed and practical culture of Oplegnathus punctatus. Masan Regio. Mari. Affair. Port Admin., pp. 44-52. (in Korean)
  15. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyo-Hak Publishing, Seoul, 360pp. (in Korean)
  16. Kim, S.Y., I.C. Bang and S.M. Kim. 2000. Sexual Maturation Inducement of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Environmental Condition. Korean J. Ichthyol., 12: 46-53. (in Korean)
  17. Kwun, H.J. and J.K. Kim. 2010. Occurrence of Natural Hybrid between Oplegnathus fasciatus and Oplegnathus punctatus from the South Sea of Korea. Korean J. Ichthyol., 22: 201-205. (in Korean)
  18. Lee, C.K., S.B. Hur, S. Park and B.G. Kim. 1997. Qualities of spawned eggs during the spawning period in red spotted grouper, Epinephelus akaare. Kor. J. Aquacult., 10: 463-472. (in Korean)
  19. Lee, W.O., K.H. Kim, J.M. Back and M.Y. Song. 2013. Egg development and early life history of Zacco koreanus (Pisces: Cyprinidae). Korean J. Ichthyol., 25: 200-207. (in Korean)
  20. Park, J.M., J.K. Cho, K.H. Han, N.R. Kim, H.K. Hwang, K.M. Kim, J.I. Myeong and M.H. Son. 2014. Early life history of the sevenband grouper, Epinephelus septemfasciatus from Korea. Dev. Reprod., 18: 13-23. (in Korean) https://doi.org/10.12717/DR.2014.18.1.013