약학회지 (YAKHAK HOEJI)

  • 제59권1호
  • /
  • Pages.1-5
  • /
  • 2015
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

밀리타리스 동충하초(Cordyceps militaris)의 인플루엔자백신 적응면역에 미치는 영향

The Effect of Cordyceps militaris on Adaptive Immune Responses in DBA2 Mice Immunized with Influenza Vaccine

  • 이환희 (덕성여자대학교 약학대학 약학과) ;
  • 조효선 (덕성여자대학교 약학대학 약학과)
  • Lee, Hwan Hee (Department of Pharmacy, College of Pharmacy, Duksung Women's University) ;
  • Cho, Hyosun (Department of Pharmacy, College of Pharmacy, Duksung Women's University)
  • 투고 : 2014.12.09
  • 심사 : 2015.02.12
  • 발행 : 2015.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cordyceps militaris has shown to have various pharmacological activities including an immune-modulatory effect. Previously, we reported that anti-influenza effect of C. militaris in DBA/2 mice was mediated by increased IL-12 and the activation of NK cells. In this study, we investigated the effect of C. militaris on adaptive immune responses using DBA2 mice immunized with influenza vaccine. To determine the effect of C. militaris on antigen presentation capability, we treated RAW 264.7 cells with various concentrations of ethanol extract of C. militaris, which showed a significant upregulation of CD86 (B7.2), CD284 (TLR4), CD40, H-2k (MHC I) and I-Ad (MHC II). To examine the direct effect of C. militaris on adaptive immune responses, we immunized DBA2 mice with influenza vaccine in presence or absence of C. militaris. After 2 or 4 weeks, influenza-specific T cell proliferation, HAI titers and IFN-${\gamma}$ production were measured in plasma or PBMCs isolated from animals. Influenza-specific T cell proliferation and HAI titers were not considerably increased in immunized mice in presence of C. militaris. However, the production of IFN-${\gamma}$ was much greater in immunized mice with C. militaris as adjuvant than only immunized mice.

키워드

참고문헌

  1. Tuli, H. S., Sharma, A. K., Sandhu, S. S. and Kashyap, D. : Cordycepin: a bioactive metabolite with therapeutic potential. Life Sci. 93, 863 (2013). https://doi.org/10.1016/j.lfs.2013.09.030
  2. Yue, K., Ye, M., Zhou, Z., Sun, W. and Lin, X. : The genus Cordyceps: a chemical and pharmacological review. J. Pharm. Pharmacol. 65, 474 (2013). https://doi.org/10.1111/j.2042-7158.2012.01601.x
  3. Zhou, X., Gong, Z., Su, Y., Lin, J. and Tang, K. : Cordyceps fungi : natural products, pharmacological functions and developmental products. J. Pharm. Pharmacol. 61, 279 (2009). https://doi.org/10.1211/jpp.61.03.0002
  4. Shin, S., Kwon, J., Lee, S., Kong, H., Lee, S., Lee, C. K., Cho, K., Ha, N. J. and Kim, K. : Immunostimulatory effects of Cordyceps militaris on macrophages through the enhanced production of cytokines via the activation of NF-kappa. Immune Netw. 10, 55 (2010). https://doi.org/10.4110/in.2010.10.2.55
  5. Chen, J. R., Yen, J. H., Lin, C. C., Tsai, W. J., Liu, W. J., Tsai, J. J., Lin, S. F. and Liu, H. W. : The effects of Chinese herbs on improving survival and inhibiting anti-ds DNA antibody production in lupus mice. Am. J. Chin. Med. 21, 257 (1993). https://doi.org/10.1142/S0192415X93000303
  6. Kang, I. S., Kim, H., Lee, T. H., Kwon, Y. S., Son, M. and Kim, C. : Effect of Cordyceps militaris on immune activity. Yakhak Hoeji 58, 81 (2014).
  7. Lee, H. H., Park, H., Sung, G. H., Lee, K., Lee, T. and Cho, H. : Anti-influenza effect of Cordyceps militaris through immunomodulation in a DBA/2 mouse model. J. Microbiol. 52, 696 (2014). https://doi.org/10.1007/s12275-014-4300-0
  8. Shin, S., Park, Y., Kim, S., Oh, H. E., Ko, Y. W., Han, S., Lee, S., Lee, C. K., Cho, K. and Kim, K. : Cordyceps militaris enhances MHC-restricted antigen presentation via the induced expression of MHC molecules and production of cytokines. Immune Netw. 10, 135 (2010). https://doi.org/10.4110/in.2010.10.4.135
  9. Manicassamy, B., Medina, R. A., Hai, R., Tsibane, T., Stertz, S., Nistal-Villan, E., Palese, P., Basler, C. F. and Garcia-Sastre, A. : Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccines, PLoS Pathog. 6, e1000745. doi: 10.1371 (2010). https://doi.org/10.1371/journal.ppat.1000745
  10. Lee, S., Kim, J. I., Heo, J., Lee, I., Park, S., Hwang, M. W., Bae, J. Y., Park, M. S., Park, H. J. and Park, M. S. : The antiinfluenza virus effect of Phellinus igniarius extract. J. Microbiol. 51, 676 (2013). https://doi.org/10.1007/s12275-013-3384-2