Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
권호 표지

Detection of Adulteration and Species Identification of Milk and Dairy Products using PCR: A Review

PCR을 이용한 품종동정 및 시유와 낙농제품의 진위판별 방법에 관한 연구: 총설

  • Choi, Suk-Ho (Division of Animal Resources and Life Science, Sangji University) ;
  • Lee, Seung-Bae (Division of Animal Resources and Life Science, Sangji University)
  • 최석호 (상지대학교 동물생명자원학부) ;
  • 이승배 (상지대학교 동물생명자원학부)
  • Received : 2015.10.17
  • Accepted : 2015.12.02
  • Published : 2015.12.31
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Abstract

The authentication and implications of misleading labeling in milk and dairy products is important to protect against cheating consumers from adulteration and to alert sensitive consumers to any undeclared potential allergens. This need to support milk and dairy products labeling has led to the development of specific analytical techniques for the analysis of milk and dairy products ingredients. Recently, several methods based on polymerase chain reaction (PCR), including restriction fragment length polymorphism (PCR-RFLP), multiplex PCR, species-specific PCR, and real-time PCR, have been proposed as useful means for identifying species of origin in milk and dairy products, as well as quantifying and detecting any adulteration. These methods have particular advantages owing to their high specificity and sensitivity, as well as rapid processing time. In this review, we provide an updated and extensive overview of the PCR-based methods used for milk and dairy products authentication with a particular focus on the application of PCR methods to detect adulteration.

Keywords

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