생체재료학회지 (Biomaterials Research)

  • 제19권3호
  • /
  • Pages.174-183
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  • 2015
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

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Biocompatibility of a polymer based on Off-Stoichiometry Thiol-Enes + Epoxy (OSTE+) for neural implants

  • 투고 : 2015.03.27
  • 심사 : 2015.09.04
  • 발행 : 2015.09.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background: The flexibility of implantable neural probes has increased during the last 10 years, starting with stiff materials such as silicone to more flexible materials like polyimide. We have developed a novel polymer based on Off-Stoichiometry Thiol-Enes + Epoxy (OSTE+, consisting of a thiol, two allyls, an epoxy resin and two initiators), which is up to 100 times more flexible than polyimide. Since a flexible neural probe should be more biocompatible than a stiff probe, an OSTE+ probe should be more biocompatible than one composed of a more rigid material. We have investigated the toxicity of OSTE+ as well as of OSTE+ that had been incubated in water for a week (OSTE+$H_2O$) using MTT assays with mouse L929 fibroblasts. We found that OSTE+ showed cytotoxicity, but OSTE+$H_2O$ did not. Extracts were analyzed using LC-MS and GC-MS in order to identify leaked chemicals. Results: Most constituents were found in extracts of OSTE+, whereas only initiators were found in OSTE+$H_2O$ extracts. The detected levels of each chemical found in the LC-MS and the GC-MS analysis were below the toxicity level when compared to MTT assays of all the individual chemicals, except for one of the initiators that had an $IC_{50}$ value close to the detected levels. Conclusion: Our notion is that the toxicity of OSTE+ was caused by one of the initiators, by impurities in the constituents or by synergistic effects of low doses of leaked chemicals. However, our conclusion is that if OSTE+ is incubated for one week in water, OSTE+ is not cytotoxic and suitable for further in vivo studies.

키워드

과제정보

연구 과제 주관 기관 : Swedish Research Council

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