생체재료학회지 (Biomaterials Research)

  • 제19권2호
  • /
  • Pages.57-66
  • /
  • 2015
  • /
  • 1226-4601(pISSN)
  • /
  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds

  • Park, Jin-Young (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Yang, Cheryl (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jung, Im-Hee (Department of Dental Hygiene, College of Health Sciences, Eulji University) ;
  • Lim, Hyun-Chang (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Lee, Jung-Seok (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jung, Ui-Won (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Seo, Young-Kwon (Department of Medical Biotechnology, Dongguk University) ;
  • Park, Jung-Keug (Department of Medical Biotechnology, Dongguk University) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry)
  • 투고 : 2015.01.04
  • 심사 : 2015.02.24
  • 발행 : 2015.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis. Result: Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds mostly unresorbed at 4 weeks. The SEM observations after cell-seeding revealed attachment of the cells onto silk fibroin with production of extracellular matrix. New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone. There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups. Conclusion: Within the limitations of this study, silk scaffold is a biocompatible material with potential expediency as an osteoconductive scaffold in bone tissue engineering. However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Health & Welfare

참고문헌

  1. Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, et al. Tissue-engineered bone regeneration. Nat Biotechnol. 2000;18(9):959-63. https://doi.org/10.1038/79449
  2. Park SY, Ki CS, Park YH, Jung HM, Woo KM, Kim HJ. Electrospun silk fibroin scaffolds with macropores for bone regeneration: an in vitro and in vivo study. Tissue Eng Part A. 2010;16(4):1271-9. https://doi.org/10.1089/ten.tea.2009.0328
  3. Kim HJ, Kim UJ, Kim HS, Li C, Wada M, Leisk GG, et al. Bone tissue engineering with premineralized silk scaffolds. Bone. 2008;42(6):1226-34. https://doi.org/10.1016/j.bone.2008.02.007
  4. Neman J, Hambrecht A, Cadry C, Jandial R. Stem cell-mediated osteogenesis: therapeutic potential for bone tissue engineering. Biologics. 2012;6:47-57.
  5. Pacifici L, Casella F, Ripari M. [The principles of tissue engineering: role of growth factors in the bone regeneration]. Minerva Stomatol. 2002;51(9):351-9.
  6. Jiang J, Hao W, Li Y, Yao J, Shao Z, Li H, et al. Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells. Biotechnol Lett. 2013;35(4):657-61. https://doi.org/10.1007/s10529-012-1121-2
  7. Thimm BW, Wust S, Hofmann S, Hagenmuller H, Muller R. Initial cell pre-cultivation can maximize ECM mineralization by human mesenchymal stem cells on silk fibroin scaffolds. Acta Biomater. 2011;7(5):2218-28. https://doi.org/10.1016/j.actbio.2011.02.004
  8. Correia C, Grayson W, Eton R, Gimble JM, Sousa RA, Reis RL, et al. Human adipose-derived cells can serve as a single-cell source for the in vitro cultivation of vascularized bone grafts. J Tissue Eng Regen Med. 2012;8(8):629-39. https://doi.org/10.1002/term.1564
  9. Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009;88(9):792-806. https://doi.org/10.1177/0022034509340867
  10. He P, Sahoo S, Ng KS, Chen K, Toh SL, Goh JC. Enhanced osteoinductivity and osteoconductivity through hydroxyapatite coating of silk-based tissue-engineered ligament scaffold. J Biomed Mater Res A. 2013;101(2):555-66.
  11. Kim BC, Bae H, Kwon IK, Lee EJ, Park JH, Khademhosseini A, et al. Osteoblastic/cementoblastic and neural differentiation of dental stem cells and their applications to tissue engineering and regenerative medicine. Tissue Eng Part B Rev. 2012;18(3):235-44. https://doi.org/10.1089/ten.teb.2011.0642
  12. Laino G, d'Aquino R, Graziano A, Lanza V, Carinci F, Naro F, et al. A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res. 2005;20(8):1394-402. https://doi.org/10.1359/JBMR.050325
  13. Yang X, Walboomers XF, van den Beucken JJ, Bian Z, Fan M, Jansen JA. Hard tissue formation of STRO-1-selected rat dental pulp stem cells in vivo. Tissue Eng Part A. 2009;15(2):367-75. https://doi.org/10.1089/ten.tea.2008.0133
  14. Graziano A, d'Aquino R, Laino G, Papaccio G. Dental pulp stem cells: a promising tool for bone regeneration. Stem Cell Rev. 2008;4(1):21-6. https://doi.org/10.1007/s12015-008-9013-5
  15. d'Aquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A, et al. Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009;18:75-83. https://doi.org/10.22203/eCM.v018a07
  16. El-Backly RM, Massoud AG, El-Badry AM, Sherif RA, Marei MK. Regeneration of dentine/pulp-like tissue using a dental pulp stem cell/poly(lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits. Aust Endod J. 2008;34(2):52-67. https://doi.org/10.1111/j.1747-4477.2008.00139.x
  17. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364(9429):149-55. https://doi.org/10.1016/S0140-6736(04)16627-0
  18. Gay IC, Chen S, MacDougall M. Isolation and characterization of multipotent human periodontal ligament stem cells. Orthod Craniofac Res. 2007;10(3):149-60. https://doi.org/10.1111/j.1601-6343.2007.00399.x
  19. Yang ZH, Zhang XJ, Dang NN, Ma ZF, Xu L, Wu JJ, et al. Apical tooth germ cell-conditioned medium enhances the differentiation of periodontal ligament stem cells into cementum/periodontal ligament-like tissues. J Periodontal Res. 2009;44(2):199-210. https://doi.org/10.1111/j.1600-0765.2008.01106.x
  20. Ge S, Zhao N, Wang L, Yu M, Liu H, Song A, et al. Bone repair by periodontal ligament stem cellseeded nanohydroxyapatite-chitosan scaffold. Int J Nanomedicine. 2012;7:5405-14.
  21. Tour G, Wendel M, Moll G, Tcacencu I. Bone repair using periodontal ligament progenitor cell-seeded constructs. J Dent Res. 2012;91(8):789-94. https://doi.org/10.1177/0022034512452430
  22. Sohn JY, Park JC, Um YJ, Jung UW, Kim CS, Cho KS, et al. Spontaneous healing capacity of rabbit cranial defects of various sizes. J Periodontal Implant Sci. 2010;40(4):180-7. https://doi.org/10.5051/jpis.2010.40.4.180
  23. Parry PV, Engh JA. High strength silk protein scaffolds: the future of spinal fusions. Neurosurgery. 2012;71(2):N29-30. https://doi.org/10.1227/01.neu.0000417541.38365.a2
  24. Qian J, Suo A, Jin X, Xu W, Xu M. Preparation and in vitro characterization of biomorphic silk fibroin scaffolds for bone tissue engineering. J Biomed Mater Res A. 2013;102(9):2961-71.
  25. Teuschl AH, van Griensven M, Redl H. Sericin removal from raw Bombyx mori silk scaffolds of high hierarchical order. Tissue Eng Part C Methods. 2013;20(5):431-9. https://doi.org/10.1089/ten.tec.2013.0278
  26. Cao Y, Wang B. Biodegradation of silk biomaterials. Int J Mol Sci. 2009;10(4):1514-24. https://doi.org/10.3390/ijms10041514
  27. Kirker-Head C, Karageorgiou V, Hofmann S, Fajardo R, Betz O, Merkle HP, et al. BMP-silk composite matrices heal critically sized femoral defects. Bone. 2007;41(2):247-55. https://doi.org/10.1016/j.bone.2007.04.186
  28. Gisep A, Wieling R, Bohner M, Matter S, Schneider E, Rahn B. Resorption patterns of calcium-phosphate cements in bone. J Biomed Mater Res A. 2003;66(3):532-40.
  29. Minoura N, Tsukada M, Nagura M. Physico-chemical properties of silk fibroin membrane as a biomaterial. Biomaterials. 1990;11(6):430-4. https://doi.org/10.1016/0142-9612(90)90100-5
  30. Wang Y, Rudym DD, Walsh A, Abrahamsen L, Kim HJ, Kim HS, et al. In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials. 2008;29(24-25):3415-28. https://doi.org/10.1016/j.biomaterials.2008.05.002
  31. Kim JY, Choi JY, Jeong JH, Jang ES, Kim AS, Kim SG, et al. Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep. 2010;43(1):52-6. https://doi.org/10.5483/BMBRep.2010.43.1.052
  32. Wang J, Wei Y, Yi H, Liu Z, Sun D, Zhao H. Cytocompatibility of a silk fibroin tubular scaffold. Mater Sci Eng C Mater Biol Appl. 2014;34:429-36. https://doi.org/10.1016/j.msec.2013.09.039
  33. Liu TL, Miao JC, Sheng WH, Xie YF, Huang Q, Shan YB, et al. Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing. J Zhejiang Univ Sci B. 2010;11(1):10-6. https://doi.org/10.1631/jzus.B0900163
  34. Pascu EI, Stokes J, McGuinness GB. Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering. Mater Sci Eng C Mater Biol Appl. 2013;33(8):4905-16. https://doi.org/10.1016/j.msec.2013.08.012
  35. Webster TJ, Siegel RW, Bizios R. Osteoblast adhesion on nanophase ceramics. Biomaterials. 1999;20(13):1221-7. https://doi.org/10.1016/S0142-9612(99)00020-4
  36. Wei K, Li Y, Kim KO, Nakagawa Y, Kim BS, Abe K, et al. Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior. J Biomed Mater Res A. 2011;97(3):272-80.
  37. Riccio M, Maraldi T, Pisciotta A, La Sala GB, Ferrari A, Bruzzesi G, et al. Fibroin scaffold repairs critical-size bone defects in vivo supported by human amniotic fluid and dental pulp stem cells. Tissue Eng Part A. 2012;18(9-10):1006-13. https://doi.org/10.1089/ten.tea.2011.0542
  38. Gronthos S, Arthur A, Bartold PM, Shi S. A method to isolate and culture expand human dental pulp stem cells. Methods Mol Biol. 2011;698:107-21. https://doi.org/10.1007/978-1-60761-999-4_9
  39. Grinnemo KH, Mansson A, Dellgren G, Klingberg D, Wardell E, Drvota V, et al. Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. J Thorac Cardiovasc Surg. 2004;127(5):1293-300. https://doi.org/10.1016/j.jtcvs.2003.07.037
  40. Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G, et al. Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation. 2005;80(6):836-42. https://doi.org/10.1097/01.tp.0000173794.72151.88

피인용 문헌

  1. Scaffolds in regenerative endodontics: A review vol.13, pp.5, 2016, https://doi.org/10.4103/1735-3327.192266
  2. Analysis of the Adherence of Dental Pulp Stem Cells on Two-Dimensional and Three-Dimensional Silk Fibroin-Based Biomaterials vol.28, pp.4, 2015, https://doi.org/10.1097/scs.0000000000003596
  3. Rabbit Calvarial Defect Model for Customized 3D-Printed Bone Grafts vol.24, pp.5, 2015, https://doi.org/10.1089/ten.tec.2017.0474
  4. Silk fibroin/hydroxyapatite composites for bone tissue engineering vol.36, pp.1, 2015, https://doi.org/10.1016/j.biotechadv.2017.10.001
  5. Use of thioglycerol on porous polyurethane as an effective theranostic capping agent for bone tissue engineering vol.33, pp.7, 2015, https://doi.org/10.1177/0885328218817173
  6. Comprehensive Review on Silk at Nanoscale for Regenerative Medicine and Allied Applications vol.5, pp.5, 2015, https://doi.org/10.1021/acsbiomaterials.8b01560
  7. Mechanical Strength and Porosity of Carbonate Apatite-Chitosan-Gelatine Scaffold in Various Ratio as a Biomaterial Candidate in Tissue Engineering vol.829, pp.None, 2015, https://doi.org/10.4028/www.scientific.net/kem.829.173
  8. Hydroxyapatite as a biomaterial - a gift that keeps on giving vol.46, pp.7, 2015, https://doi.org/10.1080/03639045.2020.1776321
  9. Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications vol.18, pp.None, 2015, https://doi.org/10.1186/s12951-019-0570-3
  10. Advances of nanomaterial applications in oral and maxillofacial tissue regeneration and disease treatment vol.13, pp.2, 2015, https://doi.org/10.1002/wnan.1669
  11. 3D-Printed Barrier Membrane Using Mixture of Polycaprolactone and Beta-Tricalcium Phosphate for Regeneration of Rabbit Calvarial Defects vol.14, pp.12, 2021, https://doi.org/10.3390/ma14123280
  12. Decellularized and biological scaffolds in dental and craniofacial tissue engineering: a comprehensive overview vol.15, pp.None, 2021, https://doi.org/10.1016/j.jmrt.2021.08.083