The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Isolation and Genomic Characterization of the T4-Like Bacteriophage PM2 Infecting Pectobacterium carotovorum subsp. carotovorum

  • Lim, Jeong-A (Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Dong Hwan (Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Heu, Sunggi (Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2014.09.29
  • 심사 : 2014.11.05
  • 발행 : 2015.03.01
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초록

In order to control Pectobacterium carotovorum subsp. carotovorum, a novel virulent bacteriophage PM2 was isolated. Bacteriophage PM2 can infect 48% of P. carotovorum subsp. carotovorum and 78% of P. carotovorum subsp. brasilliensis but none of atrosepticum, betavasculorum, odoriferum and wasabiae isolates had been infected with PM2. PM2 phage belongs to the family Myoviridae, and contains a large head and contractile tail. It has a 170,286 base pair genome that encodes 291 open reading frames (ORFs) and 12 tRNAs. Most ORFs in bacteriophage PM2 share a high level of homology with T4-like phages including IME08, RB69, and JS98. Phylogenetic analysis based on the amino acid sequence of terminase large subunits confirmed that PM2 is classified as a T4-like phage. It contains no integrase- or no repressor-coding genes related to the lysogenic cycle, and lifestyle prediction using PHACT software suggested that PM2 is a virulent bacteriophage.

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참고문헌

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피인용 문헌

  1. Genomic characterization of bacteriophage vB_PcaP_PP2 infecting Pectobacterium carotovorum subsp. carotovorum, a new member of a proposed genus in the subfamily Autographivirinae vol.162, pp.8, 2017, https://doi.org/10.1007/s00705-017-3349-6
  2. Bacteriophages of Soft RotEnterobacteriaceae—a minireview vol.363, pp.2, 2016, https://doi.org/10.1093/femsle/fnv230