Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Anti-obese effects of mulberry (Morus alba L.) root bark through the inhibition of digestive enzymes and 3T3-L1 adipocyte differentiation

소화효소 저해 및 지방세포 분화 억제활성에 의한 상백피의 항비만 효능

  • Wu, Yong-Xiang (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, You-Jeong (Department of Food Science and Biotechnology, Andong National University) ;
  • Li, Sha (Department of Food Science and Biotechnology, Andong National University) ;
  • Yun, Myung-Chul (Department of Food Science and Biotechnology, Andong National University) ;
  • Yoon, Jin-Mi (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Jin-Young (Department of Food Science and Biotechnology, Andong National University) ;
  • Cho, Sung-Il (Department of Food Science and Biotechnology, Andong National University) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Kim, Taewan (Department of Food Science and Biotechnology, Andong National University)
  • 우용시앙 (안동대학교 식품생명공학과) ;
  • 김유정 (안동대학교 식품생명공학과) ;
  • 리샤 (안동대학교 식품생명공학과) ;
  • 윤명철 (안동대학교 식품생명공학과) ;
  • 윤진미 (안동대학교 식품생명공학과) ;
  • 김진영 (안동대학교 식품생명공학과) ;
  • 조성일 (안동대학교 식품생명공학과) ;
  • 손건호 (안동대학교 식품영양학과) ;
  • 김태완 (안동대학교 식품생명공학과)
  • Received : 2014.12.02
  • Accepted : 2015.01.27
  • Published : 2015.02.28
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Abstract

Anti-obese effects of mulberry (Morus alba L.) root bark was investigated in vitro by measuring its inhibitory effect against 3T3-L1 preadipocyte differentiation and digestive enzymes such as ${\alpha}$-amylase, ${\alpha}$-glucosidase and pancreatic lipase. Ethanol extract of mulberry root bark (MRE) showed the potent inhibitory activities on ${\alpha}$-amylase, ${\alpha}$-glucosidase and pancreatic lipase with $IC_{50}$ values of $7.86{\pm}0.36$, $0.12{\pm}0.03$ and $7.93{\pm}0.11mg/mL$, respectively. Furthermore, MRE significantly suppressed cellular lipid accumulation in 3T3-L1 cells in a dose-dependent manner. To elucidate the mechanism of MRE, we performed qRT-PCR and Western blotting for the expression of genes related with adipogenesis and lipogenesis. Treatment of MRE markedly suppressed the protein expression of $PPAR{\gamma}$, $C/EBP{\alpha}$ and SREBP-1c, as well as FAS and ACC, which are the key transcription factors and metabolic enzymes in adipogenesis and lipogenesis. In addition, qRT-PCR analysis indicated that the anti-adipogenesis effect of MRE might be due to its inhibition at transcription levels. These results demonstrate that MRE can effectively suppress adipocyte differentiation and inhibit key enzymes related to obesity. Our findings suggest that mulberry root bark may have a potential benefit in preventing obesity.

본 연구에서는 상백피의 소화효소 저해활성과 3T3-L1 전지방세포의 분화 억제능을 기반으로 항비만 효능소재로서의 활용가능성을 평가하였다. 상백피의 에탄올 추출물(MRE)은 ${\alpha}$-amylase와 ${\alpha}$-glucosidase, pancreatic lipase를 활용한 소화효소 저해활성 평가 실험에서 각각 $7.86{\pm}0.36$, $0.12{\pm}0.03$, $7.93{\pm}0.11mg/mL$의 $IC_{50}$ 값을 보이며 우수한 억제 활성을 나타냈다. 또한 3T3-L1 전지방세포를 활용한 세포분화억제효능실험에서 MRE 처리군의 세포내 지방 축적율은 농도 의존적으로 감소되었다. 상백피의 항비만 작용 기전을 구명하기 위하여 adipogenesis 및 lipogenesis와 관련된 유전자 발현양상을 분석한 결과, 상백피 추출물 처리군에서는 생체내 지방대사 조절에 중요한 역할을 하는 FAS와 ACC 뿐 아니라 adipogenesis와 lipogenesis와 관련된 주요 전사요소인 $PPAR{\gamma}$와 $C/EBP{\alpha}$, SREBP-1c의 유전자 발현이 현저하게 억제되었다. qRT-PCR 분석 결과, 상백피 추출물의 anti-adipogenesis 효능은 전사단계에서의 관련 유전자 발현억제에 기인한다고 판단되었다. 본 실험결과 상백피 추출물은 전지방세포의 분화와 세포내 지질합성을 저해하고 비만과 관련 된 소화효소에 대한 저해활성을 나타내었다. 이러한 결과를 기반으로 상백피의 비만 예방 소재로서의 잠재적인 가능성을 확인하였다.

Keywords

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