Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Quality Characteristics of Seasoned Pork with Water Extracts of Allium hookeri Root during Storage

Allium hookeri 뿌리 열수 추출물을 첨가한 양념돈육의 저장 중 품질 특성

  • Park, Min-Young (Department of Food and Nutrition, Chungnam National University) ;
  • Ly, Sun Yung (Department of Food and Nutrition, Chungnam National University)
  • 박민영 (충남대학교 식품영양학과) ;
  • 이선영 (충남대학교 식품영양학과)
  • Received : 2014.10.10
  • Accepted : 2015.01.20
  • Published : 2015.02.28
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Abstract

This study examined the antioxidant and antimicrobial activities of 70% ethanol extracts and water extracts of Allium hookeri root. We evaluated the effects of water extracts of A. hookeri root on storage of seasoned pork added with water extracts at $4^{\circ}C$ for 21 days in order to evaluate its potential as a functional food material. A. hookeri root water extracts displayed antioxidant activities (total polyphenol content and DPPH and ABTS radical scavenging activities) that were superior to those of 70% ethanol extracts. The 70% ethanol extracts and water extracts of A. hookeri root showed antimicrobial activities against food-borne Staphylococcus aureus bacteria that were about 1/400 times greater than that of vancomycin. Chemical composition analysis was conducted on pork seasoned with sauce containing 5%, 10%, and 15% water extracts. Moisture and crude ash contents significantly decreased as the amount of water extracts increased (P<0.05), and the highest crude protein content was in the 10% group. Acidity of seasoned pork increased proportionally in the early stages of storage, whereas it significantly decreased as the amount of water extracts increased after day 12 (P<0.05). Although the total number of bacteria in seasoned pork continuously increased during storage, growth of bacteria was significantly restricted as level of A. hookeri root water extracts increased (P<0.05). In the sensory evaluation, pork seasoned with 10% A. hookeri root water extracts showed the highest scores for taste, texture, and overall acceptance (P<0.05). In summary, A. hookeri water extracts display antioxidant and antimicrobial activities that can improve quality characteristics of seasoned pork and have potential as natural preservatives to restrict bacteria growth. Regarding the amount of extracts, 10% was determined to be the most appropriate level to minimize changes in seasoned pork during storage and improve sensory quality.

Allium hookeri 뿌리 70% 에탄올 및 열수 추출물의 총 폴리페놀 함량은 각각 $2.51{\pm}0.04mg/g$, $3.09{\pm}0.06mg/g$으로 열수 추출물에서 더 높았고, DPPH 및 ABTS radical 소거능도 열수 추출물에서 높은 항산화 활성을 보였다. A. hookeri 뿌리 추출물의 항균 활성을 측정한 결과, 식품부패미생물인 Staphylococcus aureus에 대해 70% 에탄올 추출물과 열수 추출물은 양성대조군인 vancomycin의 1/400배의 항균 활성을 나타내었다. 추출물 중 생리활성이 더 우수한 A. hookeri 뿌리 열수 추출물을 소스에 5, 10, 15% 첨가하여 양념돈육을 제조한 후 $4^{\circ}C$에서 21일간 냉장 저장하면서 양념돈육의 저장성과 미생물학적 관능적 특성에 미치는 영향을 검토한 결과, 수분과 조회분 함량은 열수 추출물의 첨가량이 증가할수록 유의하게 감소하였고 단백질 함량은 10% 첨가군에서 가장 높았다. 양념돈육의 산도는 저장 초기에 첨가량이 증가할수록 증가하다가 저장 12일째부터 첨가량이 증가할수록 유의적으로 감소하였다. 저장기간 동안 미생물 수는 대조군에서 가장 빠르게 증가하였고 추출물의 첨가량이 증가할수록 유의적으로 미생물 생육을 억제하였다. 또한 관능평가에서는 A. hookeri 뿌리 열수 추출물을 10% 첨가했을 때 맛, 질감, 전반적인 기호도 면에서 가장 높은 평가를 받았다. 따라서 항산화 및 항균 활성을 가진 A. hookeri 뿌리 열수 추출물은 양념돈육에서 저장성 향상 및 관능적 특성의 개선에 도움을 주며, 미생물 생육을 억제하여 천연보존제로서도 탁월한 소재라고 사료된다.

Keywords

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