Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Inhibitory Effects of Yuzu and Its Components on Human Platelet Aggregation

  • Kim, Tae-Ho (College of Pharmacy, Ajou University) ;
  • Kim, Hye-Min (College of Pharmacy, Ajou University) ;
  • Park, Se Won (Department of Molecular Biotechnology, College of Life and Environmental Sciences, Konkuk University) ;
  • Jung, Yi-Sook (College of Pharmacy, Ajou University)
  • Received : 2015.01.16
  • Accepted : 2015.02.09
  • Published : 2015.03.01
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Abstract

Our previous study demonstrated that yuzu has an anti-platelet effect in rat blood. In the present study, we examined whether the anti-platelet effect of yuzu can be extended to human blood by investigating its ability to inhibit aggregations induced by various agonists in human platelet rich plasma (PRP). This study also investigated the underlying mechanism of yuzu focusing on ADP granule secretion, $TXB_2$ formations, and $PLC{\gamma}$/Akt signaling. The results from this study showed that ethanolic yuzu extract (YE), and its components, hesperidin and naringin, inhibited human platelet aggregation in a concentration-dependent manner. YE, hesperidin and naringin also inhibited $TXB_2$ formation and ADP release. The phosphorylation of $PLC{\gamma}$ and Akt was significantly inhibited by YE, heperidin and naringin. Furthermore, we demonstrated that YE, heperidin and naringin has anti-platelet effects in rat ex vivo studies, and lower side effects in mice tail bleeding time studies. The results from this study suggest that YE, hesperidin and naringin can inhibit human platelet aggregation, at least partly through the inhibition of $PLC{\gamma}$ and Akt, leading to a decrease in $TXB_2$ formation and granule secretion.

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References

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