Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Cardamonin Suppresses TGF-β1-Induced Epithelial Mesenchymal Transition via Restoring Protein Phosphatase 2A Expression

  • Kim, Eun Ji (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University) ;
  • Kim, Hyun Ji (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University) ;
  • Park, Mi Kyung (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University) ;
  • Kang, Gyeung Jin (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University) ;
  • Byun, Hyun Jung (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University) ;
  • Lee, Ho (National Cancer Center) ;
  • Lee, Chang Hoon (BK21PLUS R-FIND Team, College of Pharmacy, Dongguk University)
  • Received : 2014.10.15
  • Accepted : 2014.12.16
  • Published : 2015.03.01
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Abstract

Epithelial mesenchymal transition (EMT) is the first step in metastasis and implicated in the phenotype of cancer stem cells. Therefore, understanding and controlling EMT, are essential to the prevention and cure of metastasis. In the present study, we examined, by Western blot, reverse transcription polymerase chain reaction (RT-PCR), and confocal microscopy, the effects of cardamonin (CDN) on transforming growth factor-${\beta}1$ (TGF-${\beta}1$)-induced EMT of A549 lung adenocarcinoma cell lines. TGF-${\beta}1$ induced expression of N-cadherin and decreased expression of E-cadherin. CDN suppressed N-cadherin expression and restored E-cadherin expression. Further, TGF-${\beta}1$ induced migration and invasion of A549 cancer cells, which was suppressed by CDN. TGF-${\beta}1$ induced c-Jun N-terminal kinase (JNK) activation during EMT, but CDN blocked it. Protein serine/threonine phosphatase 2A (PP2A) expression in A549 cancer cells was reduced by TGF-${\beta}1$ but CDN restored it. The overall data suggested that CDN suppresses TGF-${\beta}1$-induced EMT via PP2A restoration, making it a potential new drug candidate that controls metastasis.

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References

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