Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Production of Enzymatic Hydrolysate Including Water-soluble Fiber from Hemicellulose Fraction of Chinese Cabbage Waste

효소적 분해에 의한 배추부산물 hemicellulose 분획으로부터 수용성 식이섬유소 함유 가수분해물의 생산

  • 박서연 (영남대학교 식품영양학과) ;
  • 윤경영 (영남대학교 식품영양학과)
  • Received : 2014.07.11
  • Accepted : 2014.11.27
  • Published : 2015.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to determine the optimal hydrolysis conditions for the production of hydrolysates, including water-soluble dietary fiber from Chinese cabbage, with commercial enzymes. The optimal pH and temperature for hydrolysis of the hemicellulose fraction were pH 5.0 and $40^{\circ}C$, and optimal enzyme concentrations were 45 units and 21 units for Shearzyme plus and Viscozyme L, respectively. The yields of the hydrolysate including the water-soluble dietary fiber from the hemicellulose fraction by Shearzyme plus and Viscozyme L were 22.64 and 24.73%, respectively, after a 72 h reaction. The molecular weight distribution of alcohol-insoluble fiber was characterized by gel chromatography; degradation of hemicellulose increased with increasing reaction time. Our results indicate that the hemicellulose fraction was degraded to water-soluble dietary fiber by enzymatic hydrolysis, and its hydrolysate could be utilized as new watersoluble food materials.

본 연구에서는 농산부산물인 배추 겉잎의 이용성을 증진시키기 위하여 배추의 불용성 식이섬유인 hemicellulose를 분획한 후 효소 처리하여 수용성 식이섬유를 함유한 가수분해물을 생산하고자 하였다. 이를 위해 다양한 pH, 온도, 기질농도 및 효소농도에서 hemicellulose 분획을 효소 처리한 후 환원당 함량을 측정한 결과, pH 5.0, $40^{\circ}C$, 효소농도 45 unit (Shearzyme plus), 21 unit Viscozyme L)의 조건으로 가수분해 했을 때 가장 높은 분해율을 나타내었다. 선정된 조건으로 72시간 가수분해하여 수율을 측정한 결과, Shearzyme plus 및 Viscozyme L 처리 시 각각 22.64%와 24.73%의 수율을 보였다. 또한 알코올 불용성 식이섬유의 겔크로마토그래피에 의한 분자량 분포를 확인한 결과, Shearzyme plus 및 Viscozyme L에 의해 생성된 식이섬유는 반응 시간이 증가함에 따라 저분자화 되는 것을 확인 할 수 있었다. 이상의 결과에서 배추 부산물 hemicellulose 분획을 효소로 가수분해함으로써 불용성 식이섬유가 저분자화 되어 수용성 식이섬유로 전환됨을 확인하였으며, 생산된 식이섬유소 함유 가수분해물은 기능성 식품 및 식품가공 분야에 다양하게 이용될 수 있을 것으로 판단된다.

Keywords

References

  1. Hur JW, Park YA, Sohn SK, Lee SM, Jung EJ, Lee KY, Kim SJ, Ha WK. Effect of yogurt enriched water-soluble fiber on functional constipation. J. Korean Soc. Coloproctol. 23: 312-320 (2007)
  2. Yangilar F. The application of dietary fibre in food industry: structural features, effects on health and definition, obtaining and analysis of dietary fibre: a review. J. Food Nutr. Res. 1: 13-23 (2013)
  3. Andon SA. Applications of soluble dietary fiber. Food Technol. 41: 74-75 (1987)
  4. Ning L, Villota R, Artz WE. Modification of corn fiber through chemical treatments in combination with twin-screw extrusion. Cereal Chem. 68: 632-636 (1991)
  5. Shim YH, Ahn GJ, Yoo CH. Characterization of salted chinese cabbage in relation to salt content, temperature and time. Korean J. Food Cook. Sci. 19: 210-215 (2003)
  6. Hwang ES, Hong E, KIm GH. Determination of bioactive compounds and anti-cancer effect from extracts of Korean cabbage and cabbage. Korean J. Food Nutr. 25: 259-265 (2012) https://doi.org/10.9799/ksfan.2012.25.2.259
  7. Ministry of Agriculture and Forestry (MAF). Statistics of vegetables production amount. Available from: http://www.mifaff.go.kr. accessed Dec. 10, 2011.
  8. Kim SH, Yang JY, Kang SA, Chun HK, Park KY. Current state and improvement for Korean Kimchi industry. Food Indus. Nutr. 12: 7-13 (2007)
  9. Shin HH, Lee SH, Park BS, Rhim TS, Hwang JK. Solubilization of whole grains by extrusion and enzyme treatment. Korean J. Food Sci. Technol. 35: 849-855 (2003)
  10. Aden A, Foust T. Technoeconomic analysis of the dilute sulfuric acid and enzymatic hydrolysis process for the conversion of corn stover to ethanol. Cellulose 16: 535-545 (2009) https://doi.org/10.1007/s10570-009-9327-8
  11. Martinez M, Yanez R, Alonso JL, Parajo JC. Chemical production of pectic oligosaccharides from orange peel wastes. Ind. Eng. Chem. Res. 49: 8470-8476 (2010) https://doi.org/10.1021/ie101066m
  12. Chena BY, Chena SW, Wang HT. Use of different alkaline pretreatments and enzyme models to improve low-cost cellulosic biomass conversion. Biomass Bioenerg. 39: 182-191 (2012) https://doi.org/10.1016/j.biombioe.2012.01.012
  13. Kim CW, Choi HJ, Han BK, Yoo SS, Kim CN, Kim BY, Baik MY. Derivatization of rice wine meal using commercial proteases and characterization of its hydrolysates. Korean J. Food Sci. Technol. 43: 729-734 (2011) https://doi.org/10.9721/KJFST.2011.43.6.729
  14. Yoon KY, Cha MH, Shin SR, Kim KS. Enzymatic production of a soluble-fibre hydrolyzate from carrot pomace and its sugar composition. Food Chem. 92: 151-157 (2005) https://doi.org/10.1016/j.foodchem.2004.07.014
  15. Mandalari G, Bennett RN, Kirby AR, Lo Curto RB, Bisignano G, Waldron KW, Faulds CB. Enzymatic hydrolysis of flavonoids and pectic oligosaccharides from bergamot (Citrus bergamia Risso) peel. J. Agr. Food Chem. 54: 8307-8313 (2007)
  16. Nilnakara S, Chiewchan N, Devahastin S. Production of antioxidant dietary fibre powder from cabbage outer leaves. Food Bioprod. Process. 87: 301-307 (2009) https://doi.org/10.1016/j.fbp.2008.12.004
  17. Choi MH, Park YH. Production of yeast biomass using waste Chinese cabbage. Biomass Bioenerg. 25: 221-226 (2003) https://doi.org/10.1016/S0961-9534(02)00194-0
  18. Choi MH, Ji GE, Koh KH, Ryu YW, Jo DH, Park YH. Use of waste Chinese cabbage as a substrate for yeast biomass production. Bioresource Technol. 83: 251-253 (2002) https://doi.org/10.1016/S0960-8524(01)00232-2
  19. Xu F, Sun JX, Liu CF, Sun RC. Comparative study of alkali- and acidic organic solvent-soluble hemicellulosic polysaccharides from sugarcane bagasse. Carbohyd. Res. 341: 253-261 (2006) https://doi.org/10.1016/j.carres.2005.10.019
  20. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428 (1959) https://doi.org/10.1021/ac60147a030
  21. Chau CF, Huang YL. Comparison of the chemical composition and physicochemical properties of different fibers prepared from the peel of Citrus sinensis L Cv. Liucheng. J. Agr. Food Chem. 51: 2615-2618 (2003) https://doi.org/10.1021/jf025919b
  22. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 (1956) https://doi.org/10.1021/ac60111a017
  23. Szwajgier D, Pielecki J, Targoski Z. The release of ferulic acid and feruloylated oligosaccharides during wort and beer production. J. Inst. Brew. 111: 372-379 (2005) https://doi.org/10.1002/j.2050-0416.2005.tb00222.x
  24. Escarnot E, Aguedo M, Paquot M. Enzymatic hydrolysis of arabinoxylans from spelt bran and hull. J. Cereal Sci. 55: 243-253 (2012) https://doi.org/10.1016/j.jcs.2011.12.009
  25. Winkler E, Foidl N, Gbitz GM, Staubmann R, Steiner W. Enzyme-supported oil extraction from Jatropha curcas seeds. Appl. Biochem. Biotech. 97: 63-65 (1997)
  26. Zheng HZ, Hwang IW, Kim SK, Lee SH, Chung SK. Optimization of carbohydrate-hydrolyzing enzyme aided polyphenol extraction from unripe apples. J. Korean Soc. Appl. Biol. Chem. 53: 342-350 (2010) https://doi.org/10.3839/jksabc.2010.053
  27. Wu J, Wu Y, Yang C, Wang Z. Enzymatic preparation and characterization of soybean oligosaccharides from okara. Procedia Eng. 37: 186-191 (2012) https://doi.org/10.1016/j.proeng.2012.04.224
  28. Yoon KY, Woodams EE, Hang YD. Enzymatic production of pentoses from the hemicellulose fraction of corn residues. LWTFood Sci. Technol. 39: 388-392 (2006)

Cited by

  1. Production of Soluble Dietary Fiber of Buckwheat Hulls by Enzymatic Depolymerzation and its Characteristics vol.48, pp.2, 2016, https://doi.org/10.9721/KJFST.2016.48.2.97
  2. Woods vol.68, pp.2267-1242, 2018, https://doi.org/10.1051/e3sconf/20186803010