Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Improved Method to Increase Conidia Production from Isolates of Different Pathotypes of Citrus Scab Pathogen Elsinoe spp.

  • Hyun, Jae-Wook (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Paudyal, Dilli Prasad (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hwang, Rok-Yeon (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • Received : 2015.06.19
  • Accepted : 2015.08.17
  • Published : 2015.09.30
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Abstract

Elsinoe fawcettii and E. australis are two currently recognized scab pathogens of citrus. E. fawcettii has at least six pathotypes while E. australis has at least two pathotypes. Colonies of E. fawcettii and E. australis do not sporulate in artificial media including potato dextrose agar (PDA). Whiteside's method has been widely used for preparing conidial inoculum in vitro. This study was carried out to develop efficient method for conidia production from artificial media. We developed a shaking method which included the following steps: 1) Colony grown on PDA was mashed with a steel spatula; 2) Mycelia fragments were cultured in 50 ml sterilized rain water in a rotary shaker-incubator (180 rpm) at $25^{\circ}C$ for 24 h: 3) The conidia suspension was filtered through two layers of cheesecloth. Average conidia production of all isolates tested using this shaking method was approximately 13.1 times higher than that from Whiteside's method in this study.

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References

  1. Carvalho, D. D. C., Alves, E., Batista, T. R. S., Camargos, R. B. and Lopes, E. A. G. L. 2008. Comparison of methodologies for conidia production by Alternaria alternata from citrus. Brazilian J. Microbiol. 39: 792-798. https://doi.org/10.1590/S1517-83822008000400036
  2. Chung, K. R. 2011. Elsinoe fawcettii and Elsinoë australis: the fungal pathogen causing citrus scab. Mol. Plant Pathol. 12: 123-135. https://doi.org/10.1111/j.1364-3703.2010.00663.x
  3. Gopal, K., Govindarajulu, B., Ramana, K. T. V., Kumar, C. S. K., Gopi, V., Sankar, T. G., Lakshmi, L. M., Lakshmi, T. N. and Sarada, G. 2014. Citrus Scab Elsinoe fawcettii: A Review. Res. Rev. J. Agri. Allied Sci. 3: 49-58.
  4. Hyun, J. W., Timmer, L. W., Lee, S. C., Yun, S. H., Ko, S. W. and Kim, K. S. 2001. Pathological characterization and molecular analysis of Elsinoe isolates causing scab diseases of citrus in Jeju Island in Korea. Plant Dis. 85: 1013-1017. https://doi.org/10.1094/PDIS.2001.85.9.1013
  5. Hyun, J. W., Yi, S. H., MacKenzie, S. J., Timmer, L. W., Kim, K. S., Kang, S. K., Kwon, H. M. and Lim, H. C. 2009. Pathotypes and genetic relationship of worldwide collections of Elsinoe spp. causing scab diseases of citrus. Phytopathology 99: 721-728. https://doi.org/10.1094/PHYTO-99-6-0721
  6. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Asia, Ames (Iowa, USA).
  7. Masangkay, R. F., Paulitz, T. C., Hallett, S. G. and Watson, A. K. 2000. Characterization of Sporulation of Alternaria alternata f. sp. Sphenocleae. Biocontrol Sci. Techn.10: 385-397. https://doi.org/10.1080/09583150050114981
  8. Peraza-Echeverria, Rodriguez-Garcia, C. M. and Zapata-Salazar, D. M., 2008. A rapid, effective method for profuse in vitro conidial production of Mycosphaerella fijiensis L. Aust. Plant Pathol. 37: 460-463. https://doi.org/10.1071/AP08042
  9. Rodrigues, T. T. M. S., Maffia, L. A., Dhingra, O. D. and Mizubuti, E. S. G., 2010. In vitro production of conidia of Alternaria solani. Trop. Plant Pathol. 35: 203-212. https://doi.org/10.1590/S1982-56762010000400001
  10. Timmer, L. W., 2000. Scab Diseases. In: G.S. Timmer LW, Graham JH (Editor), Compendium of citrus diseases. The American Phytophathological Society, APS Press, pp. 31-32.
  11. Whiteside, J. O. 1975. Biological characteristics of Elsinoe fawcettii pertaining to the epidemiology of sour orange scab. Phytopathology 65: 1170-1175. https://doi.org/10.1094/Phyto-65-1170

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