식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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길항세균 Bacillus sp. KBC1004를 이용한 고추탄저병의 생물학적 방제제 개발

Development of Biofungicide Using Bacillus sp. KBC1004 for the Control of Anthracnose of Red Pepper

  • 강훈석 ((주)한국바이오케미칼 부설 연구소) ;
  • 강재곤 ((주)한국바이오케미칼 부설 연구소) ;
  • 박정찬 ((주)한국바이오케미칼 부설 연구소) ;
  • 이영의 ((주)한국바이오케미칼 부설 연구소) ;
  • 정윤우 ((주)한국바이오케미칼 부설 연구소) ;
  • 김정준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 박창석 ((주)한국바이오케미칼 부설 연구소)
  • Kang, Hoon-Serg (Korea Biochemical Co. Ltd., Attached Research Institute) ;
  • Kang, Jae-Gon (Korea Biochemical Co. Ltd., Attached Research Institute) ;
  • Park, Jeong-Chan (Korea Biochemical Co. Ltd., Attached Research Institute) ;
  • Lee, Young-Ui (Korea Biochemical Co. Ltd., Attached Research Institute) ;
  • Jeong, Yoon-Woo (Korea Biochemical Co. Ltd., Attached Research Institute) ;
  • Kim, Jeong-Jun (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Chang-Seuk (Korea Biochemical Co. Ltd., Attached Research Institute)
  • 투고 : 2015.05.08
  • 심사 : 2015.07.02
  • 발행 : 2015.09.30
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초록

고추 재배토양에서 분리한 세균 중에서 고추탄저병균의 균사생장을 강하게 억제하며 분생포자의 발아를 현저히 억제하는 균주를 선발하였다. 이 균의 형태적 생리적 실험과 16S rRNA 분석결과를 토대로 Bacillus sp KBC1004로 명명하였다. KBC1004를 동결 건조하여 얻은 건조물을 원제로 하여 탄수화물과 증량제를 포함하는 수화제형($2{\times}10^8cfu/g$)으로 제조하였을 때 상온에서 2년 이상 안정된 밀도를 유지하였다. 수화제 형태의 KBC1004가 활성화 되어 탄저병균 분생포자의 발아를 억제하려면 최소 24시간이 경과되어야 하였고 실내에서 고추 열매를 이용하여 탄저병의 발병 억제력을 조사한 결과도 24시간 전에 KBC1004 수화제를 살포하고 병원균을 접종하였을 때 효과적인 병 방제가 이루어졌다. 고추포장에서 KBC1004 수화제를 500배로 희석하여 탄저병이 발생하는 6월 말부터 4회 처리하고 10일 경과 후 이병과율을 조사한 결과 화학농약의 방제가가 86.3%이였을 때 80.3%의 높은 방제가를 나타내었다.

To develop an effective biopesticide to control pepper anthracnose disease, an isolate which showed strong inhibitory effect on the mycelial growth and conidial germination of Colletotrichum acutatum was selected among the antagonistic bacterial isolates collected from pepper grown soil. The bacterial isolate was identified as Bacillus sp. KBC1004 using 16S rRNA sequence analysis. The liquid culture of KBC1004 was freeze-dried and formulated as a wettable powder(WP). The wettable powder form of KBC1004 required at least 24 hours to activate and to inhibit the conidial germination of C. acutatum. In vitro bioassay using the detached green pepper fruits, biocontrol activity of the WP was not recognizable in simultaneous inoculation, but significant disease suppression was observed pre-treatment (24 hr) of the WP before pathogen inoculation. In field experiment, 4 times foliar applications of the 1/500 diluted wettable powder from the end of June showed great control efficacy similar to that of the chemical fungicide application. These results suggest that the formulated WP product could be an alternative mean to control of pepper anthracnose disease in environmentally friendly farming practices.

키워드

참고문헌

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피인용 문헌

  1. Selection of Antifungal Bacteria Burkholderia lata CAB13001 for Control on Red Pepper Anthracnose and Its Control Efficacy in Field vol.26, pp.4, 2018, https://doi.org/10.11625/KJOA.2018.26.4.649