Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells

  • Liu, Chun-Ju (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) ;
  • Zhang, Xia-Li (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) ;
  • Luo, Da-Ya (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) ;
  • Zhu, Wei-Feng (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) ;
  • Wan, Hui-Fang (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University) ;
  • Yang, Jun-Ping (Clinical Laboratory of Jiangxi University of Traditional Chinese Medicine) ;
  • Yang, Xiao-Jun (Clinical Laboratory of Jiangxi University of Traditional Chinese Medicine) ;
  • Wan, Fu-Sheng (Department of Biochemistry and Molecular Biology, Basic Medical College, Nan Chang University)
  • Published : 2015.02.25
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Abstract

Purpose: To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. Materials and Methods: A549 cells were divided into the following groups: control, non-carrier (NC), PUMA (transfected with pCEP4-(HA) 2-PUMA plasmid), DDP ($10{\mu}g/mL$ cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and $10{\mu}g/mL$ cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. Results: Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p<0.01), with the largest reduction in the PUMA+DDP group. Conversely, the apoptosis rates of the three groups were significantly increased (P<0.01), and the PUMA and DDP treatments were synergistic. Moreover, Bax protein levels significantly increased (p<0.01), while Bcl-2 protein levels significantly decreased (p<0.01). Finally, both the volume and the weights of transplanted tumors were significantly reduced (p<0.01), and the inhibition ratio of the PUMA+DDP group was significantly higher than in the single DDP or PUMA groups. Conclusions: Exogenous PUMA effectively inhibited lung cancer A549 cell proliferation and transplanted tumor growth by increasing Bax protein levels and reducing Bcl-2 protein levels.

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References

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