The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory Effects of Gamiyunjo-tang on Lipopolysaccharide Induced Inflammatory Responses in RAW 264.7 Cells

가미윤조탕(加味潤燥湯)이 LPS로 유도된 RAW 264.7 대식세포에서의 항염 효과 연구

  • Choi, Jong-Min (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Semyung University) ;
  • Kim, Yong-Min (Dept. of Oriental Medical and Herbal Cosmetic Sciences, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Semyung University)
  • 최종민 (세명대학교 한의과대학 안이비인후피부과학 교실) ;
  • 김용민 (세명대학교 한방화장품과학과) ;
  • 김희택 (세명대학교 한의과대학 안이비인후피부과학 교실)
  • Received : 2015.01.19
  • Accepted : 2015.02.09
  • Published : 2015.02.25
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Abstract

Objectives : Allergic disease has been well known as an IgE-dependent immunologic response. Recently, interest about the late inflammatory reaction has grown up as well as early allergic reaction characterized by IgE and mast cell. The purpose of this study was to find the anti-inflammatory effect of Gamiyunjo-tang(GMYJT) in allergic reaction. Methods : The experiment was performed using Raw 264.7 cells pretreated with GMYJT extracts. In this study, we observed the toxicity of cells by MTT analysis and measured the production of LPS-induced NO, $PGE_2$, IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ at a concentration of 50, 100, 200 and $400{\mu}g/ml$. Results : No toxicity of GMYJT (50, 100, 200, $400{\mu}g/ml$) on RAW 264.7 cells was found after 24 hours incubation. LPS-induced NO production was reduced after treatment with GMYJT (100, 200, $400{\mu}g/ml$)(P<0.05). $PGE_2$ was reduced after treatment with GMYJT (100, 200, $400{\mu}g/ml$)(P<0.05). IL-$1{\beta}$ did not decrease at any dose. IL-6 decreased at 200, $400{\mu}g/ml$(P<0.05). TNF-${\alpha}$ production decreased only at $400{\mu}g/ml$(P<0.05). Conclusions : These data suggest that GMYJT has anti-inflammatory effects in late allergic reaction.

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References

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