The Journal of Advanced Prosthodontics

  • 제7권1호
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  • Pages.21-26
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  • 2015
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  • 2005-7806(pISSN)
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  • 2005-7814(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
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Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis

  • Malkoc, Meral Arslan (Department of Prosthodontics, Faculty of Dentistry, Inonu University) ;
  • Demir, Necla (Department of Prosthodontics, Faculty of Dentistry, Selcuk University) ;
  • Sengun, Abdulkadir (Department of Restorative Dentistry, Faculty of Dentistry, Kirikkale University) ;
  • Bozkurt, Serife Buket (Research Center, Faculty of Dentistry, Selcuk University) ;
  • Hakki, Sema Sezgin (Department of Periodontology, Faculty of Dentistry, Selcuk University)
  • 투고 : 2014.05.07
  • 심사 : 2014.12.03
  • 발행 : 2015.02.27
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초록

PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding $200{\mu}L$ of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.

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참고문헌

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